Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays

Abstract The effect of Plant Growth Promoting Rhizobacteria (Bacillus sp.) and silver nanoparticles on Zea mays was evaluated. The silver nanoparticles were synthesized from Tagetes erecta (Marigold) leaf and flower extracts, whereas PGPR isolated from spinach rhizosphere. The silver nanoparticles (...

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Autores principales:	Pankaj Kumar, Vikas Pahal, Arti Gupta, Ruchi Vadhan, Harish Chandra, Ramesh Chandra Dubey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a19c7d8448540119592ba608c6f8052
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spelling	oai:doaj.org-article:5a19c7d8448540119592ba608c6f80522021-12-02T11:40:41ZEffect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays10.1038/s41598-020-77460-w2045-2322https://doaj.org/article/5a19c7d8448540119592ba608c6f80522020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77460-whttps://doaj.org/toc/2045-2322Abstract The effect of Plant Growth Promoting Rhizobacteria (Bacillus sp.) and silver nanoparticles on Zea mays was evaluated. The silver nanoparticles were synthesized from Tagetes erecta (Marigold) leaf and flower extracts, whereas PGPR isolated from spinach rhizosphere. The silver nanoparticles (AgNPs) were purified using ultra centrifugation and were characterized using UV–Vis spectroscopy at gradient wavelength and also by High Resolution Transmission Electron microscopy (HRTEM). The average particles size of AgNPs was recorded approximately 60 nm. Almost all potential isolates were able to produce Indole Acetic Acid (IAA), ammonia and Hydrogen cyanide (HCN), solubilized tricalcium phosphate and inhibited the growth of Macrophomina phaseolina in vitro but the isolate LPR2 was found the best among all. On the basis of 16S rRNA gene sequence, the isolate LPR2 was characterized as Bacillus cereus LPR2. The maize seeds bacterized with LPR2 and AgNPs individually showed a significant increase in germination (87.5%) followed by LPR2 + AgNPs (75%). But the maximum growth of root and shoot of maize plant was observed in seeds coated with LPR2 followed by AgNPs and a combination of both. Bacillus cereus LPR2 and silver nanoparticles enhanced the plant growth and LPR2 strongly inhibited the growth of deleterious fungal pathogen. Therefore, LPR2 and AgNPs could be utilized as bioinoculant and growth stimulator, respectively for maize.Pankaj KumarVikas PahalArti GuptaRuchi VadhanHarish ChandraRamesh Chandra DubeyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pankaj Kumar Vikas Pahal Arti Gupta Ruchi Vadhan Harish Chandra Ramesh Chandra Dubey Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays
description	Abstract The effect of Plant Growth Promoting Rhizobacteria (Bacillus sp.) and silver nanoparticles on Zea mays was evaluated. The silver nanoparticles were synthesized from Tagetes erecta (Marigold) leaf and flower extracts, whereas PGPR isolated from spinach rhizosphere. The silver nanoparticles (AgNPs) were purified using ultra centrifugation and were characterized using UV–Vis spectroscopy at gradient wavelength and also by High Resolution Transmission Electron microscopy (HRTEM). The average particles size of AgNPs was recorded approximately 60 nm. Almost all potential isolates were able to produce Indole Acetic Acid (IAA), ammonia and Hydrogen cyanide (HCN), solubilized tricalcium phosphate and inhibited the growth of Macrophomina phaseolina in vitro but the isolate LPR2 was found the best among all. On the basis of 16S rRNA gene sequence, the isolate LPR2 was characterized as Bacillus cereus LPR2. The maize seeds bacterized with LPR2 and AgNPs individually showed a significant increase in germination (87.5%) followed by LPR2 + AgNPs (75%). But the maximum growth of root and shoot of maize plant was observed in seeds coated with LPR2 followed by AgNPs and a combination of both. Bacillus cereus LPR2 and silver nanoparticles enhanced the plant growth and LPR2 strongly inhibited the growth of deleterious fungal pathogen. Therefore, LPR2 and AgNPs could be utilized as bioinoculant and growth stimulator, respectively for maize.
format	article
author	Pankaj Kumar Vikas Pahal Arti Gupta Ruchi Vadhan Harish Chandra Ramesh Chandra Dubey
author_facet	Pankaj Kumar Vikas Pahal Arti Gupta Ruchi Vadhan Harish Chandra Ramesh Chandra Dubey
author_sort	Pankaj Kumar
title	Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays
title_short	Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays
title_full	Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays
title_fullStr	Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays
title_full_unstemmed	Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays
title_sort	effect of silver nanoparticles and bacillus cereus lpr2 on the growth of zea mays
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5a19c7d8448540119592ba608c6f8052
work_keys_str_mv	AT pankajkumar effectofsilvernanoparticlesandbacilluscereuslpr2onthegrowthofzeamays AT vikaspahal effectofsilvernanoparticlesandbacilluscereuslpr2onthegrowthofzeamays AT artigupta effectofsilvernanoparticlesandbacilluscereuslpr2onthegrowthofzeamays AT ruchivadhan effectofsilvernanoparticlesandbacilluscereuslpr2onthegrowthofzeamays AT harishchandra effectofsilvernanoparticlesandbacilluscereuslpr2onthegrowthofzeamays AT rameshchandradubey effectofsilvernanoparticlesandbacilluscereuslpr2onthegrowthofzeamays
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Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays

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