LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis

Abstract Background Ovarian cancer (OC) is characterized by early metastasis and poor prognosis, which threatens the health of women worldwide. Small nucleolar RNA host gene 20 (SNHG20), a long noncoding RNA (lncRNA), has been verified to be significantly up-regulated in several tumors, including OC...

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Autores principales:	Qi Yang, Yu-Jie Dong
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	SNHG20 Ovarian cancer microRNA-148a ROCK1 Gynecology and obstetrics RG1-991
Acceso en línea:	https://doaj.org/article/5a1d5063b4cb498594c732044983a30c
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spelling	oai:doaj.org-article:5a1d5063b4cb498594c732044983a30c2021-11-28T12:29:25ZLncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis10.1186/s13048-021-00889-81757-2215https://doaj.org/article/5a1d5063b4cb498594c732044983a30c2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13048-021-00889-8https://doaj.org/toc/1757-2215Abstract Background Ovarian cancer (OC) is characterized by early metastasis and poor prognosis, which threatens the health of women worldwide. Small nucleolar RNA host gene 20 (SNHG20), a long noncoding RNA (lncRNA), has been verified to be significantly up-regulated in several tumors, including OC. MicroRNA-148a (miR-148a)/rho-kinase1 (ROCK1) axis plays an important role in the modulation of tumor development. However, whether SNHG20 can regulate OC progression through miR-148a/ROCK1 axis remains unclear. Normal human ovarian epithelial cell line and four OC cell lines were adopted for in vitro experiments. Real-time PCR was performed to assess the levels of SNHG20 and miR-148a. OC cell proliferation, apoptosis, invasion and migration were detected using clone formation, flow cytometry, transwell, and wound healing assays, respectively. Tumor xenograft assay was applied to evaluate the effect of SNHG20 on tumor growth in vivo. Results Significant higher expression of SNHG20 was observed in OC cell lines. SNHG20 markedly promoted the invasion, migration, proliferation and inhibited the apoptosis of OC cells. SNHG20 enhanced ROCK1 expression by sponging miR-148a, and the direct binding between SNHG20/ROCK1 and miR-148a was identified. Conclusion SNHG20 promoted invasion and migration of OC via targeting miR-148a/ROCK1 axis. The present research may provide a novel insight for the therapeutic strategies of OC.Qi YangYu-Jie DongBMCarticleSNHG20Ovarian cancermicroRNA-148aROCK1Gynecology and obstetricsRG1-991ENJournal of Ovarian Research, Vol 14, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SNHG20 Ovarian cancer microRNA-148a ROCK1 Gynecology and obstetrics RG1-991
spellingShingle	SNHG20 Ovarian cancer microRNA-148a ROCK1 Gynecology and obstetrics RG1-991 Qi Yang Yu-Jie Dong LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis
description	Abstract Background Ovarian cancer (OC) is characterized by early metastasis and poor prognosis, which threatens the health of women worldwide. Small nucleolar RNA host gene 20 (SNHG20), a long noncoding RNA (lncRNA), has been verified to be significantly up-regulated in several tumors, including OC. MicroRNA-148a (miR-148a)/rho-kinase1 (ROCK1) axis plays an important role in the modulation of tumor development. However, whether SNHG20 can regulate OC progression through miR-148a/ROCK1 axis remains unclear. Normal human ovarian epithelial cell line and four OC cell lines were adopted for in vitro experiments. Real-time PCR was performed to assess the levels of SNHG20 and miR-148a. OC cell proliferation, apoptosis, invasion and migration were detected using clone formation, flow cytometry, transwell, and wound healing assays, respectively. Tumor xenograft assay was applied to evaluate the effect of SNHG20 on tumor growth in vivo. Results Significant higher expression of SNHG20 was observed in OC cell lines. SNHG20 markedly promoted the invasion, migration, proliferation and inhibited the apoptosis of OC cells. SNHG20 enhanced ROCK1 expression by sponging miR-148a, and the direct binding between SNHG20/ROCK1 and miR-148a was identified. Conclusion SNHG20 promoted invasion and migration of OC via targeting miR-148a/ROCK1 axis. The present research may provide a novel insight for the therapeutic strategies of OC.
format	article
author	Qi Yang Yu-Jie Dong
author_facet	Qi Yang Yu-Jie Dong
author_sort	Qi Yang
title	LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis
title_short	LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis
title_full	LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis
title_fullStr	LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis
title_full_unstemmed	LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis
title_sort	lncrna snhg20 promotes migration and invasion of ovarian cancer via modulating the microrna-148a/rock1 axis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/5a1d5063b4cb498594c732044983a30c
work_keys_str_mv	AT qiyang lncrnasnhg20promotesmigrationandinvasionofovariancancerviamodulatingthemicrorna148arock1axis AT yujiedong lncrnasnhg20promotesmigrationandinvasionofovariancancerviamodulatingthemicrorna148arock1axis
_version_	1718407954546294784

LncRNA SNHG20 promotes migration and invasion of ovarian cancer via modulating the microRNA-148a/ROCK1 axis

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