Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.

Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and st...

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Autores principales:	Fan Yang, Daniel S Andersen, Steven Trabue, Angela D Kent, Laura M Pepple, Richard S Gates, Adina S Howe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a23a90f28e34f5b8e5d20b7fe7c5a20
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spelling	oai:doaj.org-article:5a23a90f28e34f5b8e5d20b7fe7c5a202021-12-02T20:18:48ZMicrobial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.1932-620310.1371/journal.pone.0254730https://doaj.org/article/5a23a90f28e34f5b8e5d20b7fe7c5a202021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254730https://doaj.org/toc/1932-6203Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and stabilization of methane. Here, we conducted a 13-month field study to survey the physical, chemical, and biological changes of pit-manure across 46 farms in Iowa. Our results showed that an increased methane production rate was associated with less digestible feed ingredients, suggesting that diet influences the storage pit's microbiome. Targeted sequencing of the bacterial 16S rRNA and archaeal mcrA genes was used to identify microbial communities' role and influence. We found that microbial communities in foaming and non-foaming manure were significantly different, and that the bacterial communities of foaming manure were more stable than those of non-foaming manure. Foaming manure methanogen communities were enriched with uncharacterized methanogens whose presence strongly correlated with high methane production rates. We also observed strong correlations between feed ration, manure characteristics, and the relative abundance of specific taxa, suggesting that manure foaming is linked to microbial community assemblage driven by efficient free long-chain fatty acid degradation by hydrogenotrophic methanogenesis.Fan YangDaniel S AndersenSteven TrabueAngela D KentLaura M PeppleRichard S GatesAdina S HowePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254730 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fan Yang Daniel S Andersen Steven Trabue Angela D Kent Laura M Pepple Richard S Gates Adina S Howe Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.
description	Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and stabilization of methane. Here, we conducted a 13-month field study to survey the physical, chemical, and biological changes of pit-manure across 46 farms in Iowa. Our results showed that an increased methane production rate was associated with less digestible feed ingredients, suggesting that diet influences the storage pit's microbiome. Targeted sequencing of the bacterial 16S rRNA and archaeal mcrA genes was used to identify microbial communities' role and influence. We found that microbial communities in foaming and non-foaming manure were significantly different, and that the bacterial communities of foaming manure were more stable than those of non-foaming manure. Foaming manure methanogen communities were enriched with uncharacterized methanogens whose presence strongly correlated with high methane production rates. We also observed strong correlations between feed ration, manure characteristics, and the relative abundance of specific taxa, suggesting that manure foaming is linked to microbial community assemblage driven by efficient free long-chain fatty acid degradation by hydrogenotrophic methanogenesis.
format	article
author	Fan Yang Daniel S Andersen Steven Trabue Angela D Kent Laura M Pepple Richard S Gates Adina S Howe
author_facet	Fan Yang Daniel S Andersen Steven Trabue Angela D Kent Laura M Pepple Richard S Gates Adina S Howe
author_sort	Fan Yang
title	Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.
title_short	Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.
title_full	Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.
title_fullStr	Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.
title_full_unstemmed	Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.
title_sort	microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5a23a90f28e34f5b8e5d20b7fe7c5a20
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Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.

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