On the Applicability of Electrophoresis for Protein Quantification

Polyacrylamide gel electrophoresis is widely used for studying proteins and protein-containing objects. However, it is employed most frequently as a qualitative method rather than a quantitative one. This paper shows the feasibility of routine digital image acquisition and mathematical processing of...

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Autores principales:	Karina Dome, Zoya Akimenko, Aleksey Bychkov, Yuri Kalambet, Oleg Lomovsky
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	electrophoresis protein mechanical treatment quantification Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/5a26a49c380d4ad9ae6f1ccfa56a8a1a
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spelling	oai:doaj.org-article:5a26a49c380d4ad9ae6f1ccfa56a8a1a2021-11-25T18:48:53ZOn the Applicability of Electrophoresis for Protein Quantification10.3390/polym132239712073-4360https://doaj.org/article/5a26a49c380d4ad9ae6f1ccfa56a8a1a2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3971https://doaj.org/toc/2073-4360Polyacrylamide gel electrophoresis is widely used for studying proteins and protein-containing objects. However, it is employed most frequently as a qualitative method rather than a quantitative one. This paper shows the feasibility of routine digital image acquisition and mathematical processing of electropherograms for protein quantification when using vertical gel electrophoresis and Chrom & Spec software. Both the well-studied model protein molecules (bovine serum albumin) and more complex real-world protein-based products (casein-containing isolate for sports nutrition), which were subjected to mechanical activation in a planetary ball mill to obtain samples characterized by different protein denaturation degrees, were used as study objects. Protein quantification in the mechanically activated samples was carried out. The degree of destruction of individual protein was shown to be higher compared to that of the protein-containing mixture after mechanical treatment for an identical amount of time. The methodological approach used in this study can serve as guidance for other researchers who would like to use electrophoresis for protein quantification both in individual form and in protein mixtures. The findings prove that photographic imaging of gels followed by mathematical data processing can be applied for analyzing the electrophoretic data as an affordable, convenient and quick tool.Karina DomeZoya AkimenkoAleksey BychkovYuri KalambetOleg LomovskyMDPI AGarticleelectrophoresisproteinmechanical treatmentquantificationOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3971, p 3971 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrophoresis protein mechanical treatment quantification Organic chemistry QD241-441
spellingShingle	electrophoresis protein mechanical treatment quantification Organic chemistry QD241-441 Karina Dome Zoya Akimenko Aleksey Bychkov Yuri Kalambet Oleg Lomovsky On the Applicability of Electrophoresis for Protein Quantification
description	Polyacrylamide gel electrophoresis is widely used for studying proteins and protein-containing objects. However, it is employed most frequently as a qualitative method rather than a quantitative one. This paper shows the feasibility of routine digital image acquisition and mathematical processing of electropherograms for protein quantification when using vertical gel electrophoresis and Chrom & Spec software. Both the well-studied model protein molecules (bovine serum albumin) and more complex real-world protein-based products (casein-containing isolate for sports nutrition), which were subjected to mechanical activation in a planetary ball mill to obtain samples characterized by different protein denaturation degrees, were used as study objects. Protein quantification in the mechanically activated samples was carried out. The degree of destruction of individual protein was shown to be higher compared to that of the protein-containing mixture after mechanical treatment for an identical amount of time. The methodological approach used in this study can serve as guidance for other researchers who would like to use electrophoresis for protein quantification both in individual form and in protein mixtures. The findings prove that photographic imaging of gels followed by mathematical data processing can be applied for analyzing the electrophoretic data as an affordable, convenient and quick tool.
format	article
author	Karina Dome Zoya Akimenko Aleksey Bychkov Yuri Kalambet Oleg Lomovsky
author_facet	Karina Dome Zoya Akimenko Aleksey Bychkov Yuri Kalambet Oleg Lomovsky
author_sort	Karina Dome
title	On the Applicability of Electrophoresis for Protein Quantification
title_short	On the Applicability of Electrophoresis for Protein Quantification
title_full	On the Applicability of Electrophoresis for Protein Quantification
title_fullStr	On the Applicability of Electrophoresis for Protein Quantification
title_full_unstemmed	On the Applicability of Electrophoresis for Protein Quantification
title_sort	on the applicability of electrophoresis for protein quantification
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5a26a49c380d4ad9ae6f1ccfa56a8a1a
work_keys_str_mv	AT karinadome ontheapplicabilityofelectrophoresisforproteinquantification AT zoyaakimenko ontheapplicabilityofelectrophoresisforproteinquantification AT alekseybychkov ontheapplicabilityofelectrophoresisforproteinquantification AT yurikalambet ontheapplicabilityofelectrophoresisforproteinquantification AT oleglomovsky ontheapplicabilityofelectrophoresisforproteinquantification
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On the Applicability of Electrophoresis for Protein Quantification

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