Bioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>

ABSTRACT Proteins secreted by the type V secretion system possess multiple functions, including the capacity to mediate adhesion, aggregation, and biolfilm formation. The type V secretion system can be divided into five subclasses, one of which is the type Ve system. Proteins of the type Ve secretio...

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Autores principales:	Kelvin G. K. Goh, Danilo G. Moriel, Steven J. Hancock, Minh-Duy Phan, Mark A. Schembri
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Escherichia coli autotransporter proteins biofilms Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5a2bce43cc6740758735a697a788792f
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spelling	oai:doaj.org-article:5a2bce43cc6740758735a697a788792f2021-11-15T15:22:27ZBioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>10.1128/mSphere.00572-192379-5042https://doaj.org/article/5a2bce43cc6740758735a697a788792f2019-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00572-19https://doaj.org/toc/2379-5042ABSTRACT Proteins secreted by the type V secretion system possess multiple functions, including the capacity to mediate adhesion, aggregation, and biolfilm formation. The type V secretion system can be divided into five subclasses, one of which is the type Ve system. Proteins of the type Ve secretion system are also referred to as inverse autotransporters (IATs). In this study, we performed an in silico analysis of 126 completely sequenced Escherichia coli genomes available in the NCBI database and identified several distinct IAT-encoding gene families whose distribution varied throughout the E. coli phylogeny. The genes included three characterized IATs (intimin, fdeC, and yeeJ) and four uncharacterized IATs (here named iatA, iatB, iatC, and iatD). The four iat genes were cloned from the completely sequenced environmental E. coli strain SMS-3-5 and characterized. Three of these IAT proteins (IatB, IatC, and IatD) were expressed at the cell surface and possessed the capacity to mediate biofilm formation in a recombinant E. coli K-12 strain. Further analysis of the iatB gene, which showed a unique association with extraintestinal E. coli strains, suggested that its regulation is controlled by the LeuO global regulator. Overall, this study provides new data describing the prevalence, sequence variation, domain structure, function, and regulation of IATs found in E. coli. IMPORTANCE Escherichia coli is one of the most prevalent facultative anaerobes of the human gut. E. coli normally exists as a harmless commensal but can also cause disease following the acquisition of genes that enhance its pathogenicity. Adhesion is an important first step in colonization of the host and is mediated by an array of cell surface components. In E. coli, these include a family of adhesins secreted by the type V secretion system. Here, we identified and characterized new proteins from an emerging subclass of the type V secretion system known as the inverse autotransporters (IATs). We found that IAT-encoding genes are present in a wide range of strains and showed that three novel IATs were localized on the E. coli cell surface and mediated biofilm formation. Overall, this study provides new insight into the prevalence, function, and regulation of IATs in E. coli.Kelvin G. K. GohDanilo G. MorielSteven J. HancockMinh-Duy PhanMark A. SchembriAmerican Society for MicrobiologyarticleEscherichia coliautotransporter proteinsbiofilmsMicrobiologyQR1-502ENmSphere, Vol 4, Iss 4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli autotransporter proteins biofilms Microbiology QR1-502
spellingShingle	Escherichia coli autotransporter proteins biofilms Microbiology QR1-502 Kelvin G. K. Goh Danilo G. Moriel Steven J. Hancock Minh-Duy Phan Mark A. Schembri Bioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>
description	ABSTRACT Proteins secreted by the type V secretion system possess multiple functions, including the capacity to mediate adhesion, aggregation, and biolfilm formation. The type V secretion system can be divided into five subclasses, one of which is the type Ve system. Proteins of the type Ve secretion system are also referred to as inverse autotransporters (IATs). In this study, we performed an in silico analysis of 126 completely sequenced Escherichia coli genomes available in the NCBI database and identified several distinct IAT-encoding gene families whose distribution varied throughout the E. coli phylogeny. The genes included three characterized IATs (intimin, fdeC, and yeeJ) and four uncharacterized IATs (here named iatA, iatB, iatC, and iatD). The four iat genes were cloned from the completely sequenced environmental E. coli strain SMS-3-5 and characterized. Three of these IAT proteins (IatB, IatC, and IatD) were expressed at the cell surface and possessed the capacity to mediate biofilm formation in a recombinant E. coli K-12 strain. Further analysis of the iatB gene, which showed a unique association with extraintestinal E. coli strains, suggested that its regulation is controlled by the LeuO global regulator. Overall, this study provides new data describing the prevalence, sequence variation, domain structure, function, and regulation of IATs found in E. coli. IMPORTANCE Escherichia coli is one of the most prevalent facultative anaerobes of the human gut. E. coli normally exists as a harmless commensal but can also cause disease following the acquisition of genes that enhance its pathogenicity. Adhesion is an important first step in colonization of the host and is mediated by an array of cell surface components. In E. coli, these include a family of adhesins secreted by the type V secretion system. Here, we identified and characterized new proteins from an emerging subclass of the type V secretion system known as the inverse autotransporters (IATs). We found that IAT-encoding genes are present in a wide range of strains and showed that three novel IATs were localized on the E. coli cell surface and mediated biofilm formation. Overall, this study provides new insight into the prevalence, function, and regulation of IATs in E. coli.
format	article
author	Kelvin G. K. Goh Danilo G. Moriel Steven J. Hancock Minh-Duy Phan Mark A. Schembri
author_facet	Kelvin G. K. Goh Danilo G. Moriel Steven J. Hancock Minh-Duy Phan Mark A. Schembri
author_sort	Kelvin G. K. Goh
title	Bioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>
title_short	Bioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>
title_full	Bioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>
title_fullStr	Bioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>
title_full_unstemmed	Bioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>
title_sort	bioinformatic and molecular analysis of inverse autotransporters from <named-content content-type="genus-species">escherichia coli</named-content>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/5a2bce43cc6740758735a697a788792f
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Bioinformatic and Molecular Analysis of Inverse Autotransporters from <named-content content-type="genus-species">Escherichia coli</named-content>

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