Berberine produces antidepressant-like effects in ovariectomized mice

Abstract Berberine has been reports to have antidepressant-like effects. However, it is seldom known whether berberine produces antidepressant-like effects in ovariectomized mice, which exhibit depressive-like responses. To examine the antidepressant-like effects of berberine in ovariectomized mice,...

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Autores principales:	Jie Fan, Bingjin Li, Tongtong Ge, Zhuo Zhang, Jiayin Lv, Jing Zhao, Pu Wang, Wei Liu, Xuefeng Wang, Katarzyna Mlyniec, Ranji Cui
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a31542bf5a74791a0744b7ea64fee3f
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spelling	oai:doaj.org-article:5a31542bf5a74791a0744b7ea64fee3f2021-12-02T11:52:59ZBerberine produces antidepressant-like effects in ovariectomized mice10.1038/s41598-017-01035-52045-2322https://doaj.org/article/5a31542bf5a74791a0744b7ea64fee3f2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01035-5https://doaj.org/toc/2045-2322Abstract Berberine has been reports to have antidepressant-like effects. However, it is seldom known whether berberine produces antidepressant-like effects in ovariectomized mice, which exhibit depressive-like responses. To examine the antidepressant-like effects of berberine in ovariectomized mice, behavioral tests were conducted, including the forced swimming test and the open field test. To elucidate the mechanisms, levels of BDNF, phosphorylated CREB and phosphorylated eEF2 were analyzed by western blotting, and c-Fos induction was examined by immunohistochemistry. In the forced swimming test, berberine decreased the immobility time in a dose-dependent manner, reversing the depressive-like effect observed in ovariectomized mice, and this effect was blocked by the 5-HT2 antagonist ketanserin. In addition, western blotting indicated that BDNF and peEF2 in the hippocampus, but not pCREB/CREB in the frontal cortex, were affected by berberine treatment. Furthermore, immunohistochemistry demonstrated that the reduction in c-Fos induced by ovariectomy were greater after berberine treatment. Ketanserin also antagonized the effect of berberine on the c-Fos expression. Our findings suggest that berberine exerts antidepressant-like effects in ovariectomized mice, and 5-HT2 receptor activation may be partially related to the antidepressant-like effects of the berberine by BDNF-CREB and eEF2 pathways.Jie FanBingjin LiTongtong GeZhuo ZhangJiayin LvJing ZhaoPu WangWei LiuXuefeng WangKatarzyna MlyniecRanji CuiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jie Fan Bingjin Li Tongtong Ge Zhuo Zhang Jiayin Lv Jing Zhao Pu Wang Wei Liu Xuefeng Wang Katarzyna Mlyniec Ranji Cui Berberine produces antidepressant-like effects in ovariectomized mice
description	Abstract Berberine has been reports to have antidepressant-like effects. However, it is seldom known whether berberine produces antidepressant-like effects in ovariectomized mice, which exhibit depressive-like responses. To examine the antidepressant-like effects of berberine in ovariectomized mice, behavioral tests were conducted, including the forced swimming test and the open field test. To elucidate the mechanisms, levels of BDNF, phosphorylated CREB and phosphorylated eEF2 were analyzed by western blotting, and c-Fos induction was examined by immunohistochemistry. In the forced swimming test, berberine decreased the immobility time in a dose-dependent manner, reversing the depressive-like effect observed in ovariectomized mice, and this effect was blocked by the 5-HT2 antagonist ketanserin. In addition, western blotting indicated that BDNF and peEF2 in the hippocampus, but not pCREB/CREB in the frontal cortex, were affected by berberine treatment. Furthermore, immunohistochemistry demonstrated that the reduction in c-Fos induced by ovariectomy were greater after berberine treatment. Ketanserin also antagonized the effect of berberine on the c-Fos expression. Our findings suggest that berberine exerts antidepressant-like effects in ovariectomized mice, and 5-HT2 receptor activation may be partially related to the antidepressant-like effects of the berberine by BDNF-CREB and eEF2 pathways.
format	article
author	Jie Fan Bingjin Li Tongtong Ge Zhuo Zhang Jiayin Lv Jing Zhao Pu Wang Wei Liu Xuefeng Wang Katarzyna Mlyniec Ranji Cui
author_facet	Jie Fan Bingjin Li Tongtong Ge Zhuo Zhang Jiayin Lv Jing Zhao Pu Wang Wei Liu Xuefeng Wang Katarzyna Mlyniec Ranji Cui
author_sort	Jie Fan
title	Berberine produces antidepressant-like effects in ovariectomized mice
title_short	Berberine produces antidepressant-like effects in ovariectomized mice
title_full	Berberine produces antidepressant-like effects in ovariectomized mice
title_fullStr	Berberine produces antidepressant-like effects in ovariectomized mice
title_full_unstemmed	Berberine produces antidepressant-like effects in ovariectomized mice
title_sort	berberine produces antidepressant-like effects in ovariectomized mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5a31542bf5a74791a0744b7ea64fee3f
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Berberine produces antidepressant-like effects in ovariectomized mice

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