An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells

Abstract Microorganisms that activate plant immune responses have attracted considerable attention as potential biocontrol agents in agriculture because they could reduce agrochemical use. However, conventional methods to screen for such microorganisms using whole plants and pathogens are generally...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mari Kurokawa, Masataka Nakano, Nobutaka Kitahata, Kazuyuki Kuchitsu, Toshiki Furuya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a32d1a4d0b3455cb513a520510282c4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5a32d1a4d0b3455cb513a520510282c4
record_format	dspace
spelling	oai:doaj.org-article:5a32d1a4d0b3455cb513a520510282c42021-12-02T18:17:53ZAn efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells10.1038/s41598-021-86560-02045-2322https://doaj.org/article/5a32d1a4d0b3455cb513a520510282c42021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86560-0https://doaj.org/toc/2045-2322Abstract Microorganisms that activate plant immune responses have attracted considerable attention as potential biocontrol agents in agriculture because they could reduce agrochemical use. However, conventional methods to screen for such microorganisms using whole plants and pathogens are generally laborious and time consuming. Here, we describe a general strategy using cultured plant cells to identify microorganisms that activate plant defense responses based on plant–microbe interactions. Microbial cells were incubated with tobacco BY-2 cells, followed by treatment with cryptogein, a proteinaceous elicitor of tobacco immune responses secreted by an oomycete. Cryptogein-induced production of reactive oxygen species (ROS) in BY-2 cells served as a marker to evaluate the potential of microorganisms to activate plant defense responses. Twenty-nine bacterial strains isolated from the interior of Brassica rapa var. perviridis plants were screened, and 8 strains that enhanced cryptogein-induced ROS production in BY-2 cells were selected. Following application of these strains to the root tip of Arabidopsis seedlings, two strains, Delftia sp. BR1R-2 and Arthrobacter sp. BR2S-6, were found to induce whole-plant resistance to bacterial pathogens (Pseudomonas syringae pv. tomato DC3000 and Pectobacterium carotovora subsp. carotovora NBRC 14082). Pathogen-induced expression of plant defense-related genes (PR-1, PR-5, and PDF1.2) was enhanced by the pretreatment with strain BR1R-2. This cell–cell interaction-based platform is readily applicable to large-scale screening for microorganisms that enhance plant defense responses under various environmental conditions.Mari KurokawaMasataka NakanoNobutaka KitahataKazuyuki KuchitsuToshiki FuruyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mari Kurokawa Masataka Nakano Nobutaka Kitahata Kazuyuki Kuchitsu Toshiki Furuya An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells
description	Abstract Microorganisms that activate plant immune responses have attracted considerable attention as potential biocontrol agents in agriculture because they could reduce agrochemical use. However, conventional methods to screen for such microorganisms using whole plants and pathogens are generally laborious and time consuming. Here, we describe a general strategy using cultured plant cells to identify microorganisms that activate plant defense responses based on plant–microbe interactions. Microbial cells were incubated with tobacco BY-2 cells, followed by treatment with cryptogein, a proteinaceous elicitor of tobacco immune responses secreted by an oomycete. Cryptogein-induced production of reactive oxygen species (ROS) in BY-2 cells served as a marker to evaluate the potential of microorganisms to activate plant defense responses. Twenty-nine bacterial strains isolated from the interior of Brassica rapa var. perviridis plants were screened, and 8 strains that enhanced cryptogein-induced ROS production in BY-2 cells were selected. Following application of these strains to the root tip of Arabidopsis seedlings, two strains, Delftia sp. BR1R-2 and Arthrobacter sp. BR2S-6, were found to induce whole-plant resistance to bacterial pathogens (Pseudomonas syringae pv. tomato DC3000 and Pectobacterium carotovora subsp. carotovora NBRC 14082). Pathogen-induced expression of plant defense-related genes (PR-1, PR-5, and PDF1.2) was enhanced by the pretreatment with strain BR1R-2. This cell–cell interaction-based platform is readily applicable to large-scale screening for microorganisms that enhance plant defense responses under various environmental conditions.
format	article
author	Mari Kurokawa Masataka Nakano Nobutaka Kitahata Kazuyuki Kuchitsu Toshiki Furuya
author_facet	Mari Kurokawa Masataka Nakano Nobutaka Kitahata Kazuyuki Kuchitsu Toshiki Furuya
author_sort	Mari Kurokawa
title	An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells
title_short	An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells
title_full	An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells
title_fullStr	An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells
title_full_unstemmed	An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells
title_sort	efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5a32d1a4d0b3455cb513a520510282c4
work_keys_str_mv	AT marikurokawa anefficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT masatakanakano anefficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT nobutakakitahata anefficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT kazuyukikuchitsu anefficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT toshikifuruya anefficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT marikurokawa efficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT masatakanakano efficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT nobutakakitahata efficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT kazuyukikuchitsu efficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells AT toshikifuruya efficientdirectscreeningsystemformicroorganismsthatactivateplantimmuneresponsesbasedonplantmicrobeinteractionsusingculturedplantcells
_version_	1718378274658189312

An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells

Ejemplares similares