Penetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method

Rob Turner,1 Sean Robert Wevrett,1 Suzanne Edmunds,1 Marc Brown,1 Anuradha Kulasekaran,2 Oluwajoba Adegoke,2 John Farrah2 1MedPharm Ltd, Guildford, UK; 2Reckitt Benckiser Healthcare International Ltd., Slough, Berkshire, UKCorrespondence: Oluwajoba AdegokeReckitt Benckiser Healthcare International L...

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Autores principales:	Turner R, Wevrett SR, Edmunds S, Brown MB, Kulasekaran A, Adegoke O, Farrah J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	flurbiprofen franz diffusion cell microautoradiography pharynx Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/5a3cb85d136d4f6895ff4346fa20b3ba
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spelling	oai:doaj.org-article:5a3cb85d136d4f6895ff4346fa20b3ba2021-12-02T10:57:13ZPenetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method1179-1438https://doaj.org/article/5a3cb85d136d4f6895ff4346fa20b3ba2021-01-01T00:00:00Zhttps://www.dovepress.com/penetration-of-flurbiprofen-from-a-locally-applied-sore-throat-lozenge-peer-reviewed-article-CPAAhttps://doaj.org/toc/1179-1438Rob Turner,1 Sean Robert Wevrett,1 Suzanne Edmunds,1 Marc Brown,1 Anuradha Kulasekaran,2 Oluwajoba Adegoke,2 John Farrah2 1MedPharm Ltd, Guildford, UK; 2Reckitt Benckiser Healthcare International Ltd., Slough, Berkshire, UKCorrespondence: Oluwajoba AdegokeReckitt Benckiser Healthcare International Ltd, Slough, Berkshire, UKTel +44 1482 582134Email joba.adegoke@rb.comObjective: Flurbiprofen 8.75 mg lozenge and spray are used for symptomatic relief of sore throat, with a rapid onset of analgesia suggesting a localized mechanism of action. Building on previous studies, this investigation aimed to use microautoradiography to visualize the depth penetration of radiolabeled flurbiprofen into human pharynx tissue using an ex vivo model. Quantification of flurbiprofen in the tissue was performed to provide a quantitative representation of flurbiprofen distribution through the tissue.Methods: Cadaveric human pharynx tissue was mounted between the donor and receiver compartments of a Franz diffusion cell. After that 8.75 mg spray and dissolved lozenge formulations, containing radiolabeled flurbiprofen, were added to the donor compartment of a Franz diffusion cell. After incubation for one hour, the pharynx tissue was removed, processed, and sectioned both horizontally and vertically. The sections were placed within an imaging cassette to determine the penetration of radiolabeled flurbiprofen visually, before being solubilized to quantify the amount of flurbiprofen present in each section.Results: In the horizontally sectioned samples, flurbiprofen was present in the top layers of all replicates and decreased in intensity throughout the tissue. Of the applied dose, 48.0– 99.9% of flurbiprofen was detected in the top one-third of the pharynx tissue, closest to the dosing site, and 0– 14.8% of flurbiprofen was detected within the deepest third of pharynx tissue, furthest from the dosing site. In the vertically sectioned tissue samples, radiolabeled flurbiprofen was found at a high intensity at the dosing site and reduced in intensity throughout the thickness of the tissue. Lateral penetration of flurbiprofen was also seen in tissue dosed with the spray.Conclusion: Our findings demonstrate that lozenge and spray formulations of flurbiprofen can penetrate throughout the layers of cadaveric human pharynx tissue in an ex vivo model, as visualized by microautoradiography.Keywords: flurbiprofen, Franz diffusion cell, microautoradiography, pharynxTurner RWevrett SREdmunds SBrown MBKulasekaran AAdegoke OFarrah JDove Medical Pressarticleflurbiprofenfranz diffusion cellmicroautoradiographypharynxTherapeutics. PharmacologyRM1-950ENClinical Pharmacology: Advances and Applications, Vol Volume 13, Pp 13-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	flurbiprofen franz diffusion cell microautoradiography pharynx Therapeutics. Pharmacology RM1-950
spellingShingle	flurbiprofen franz diffusion cell microautoradiography pharynx Therapeutics. Pharmacology RM1-950 Turner R Wevrett SR Edmunds S Brown MB Kulasekaran A Adegoke O Farrah J Penetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method
description	Rob Turner,1 Sean Robert Wevrett,1 Suzanne Edmunds,1 Marc Brown,1 Anuradha Kulasekaran,2 Oluwajoba Adegoke,2 John Farrah2 1MedPharm Ltd, Guildford, UK; 2Reckitt Benckiser Healthcare International Ltd., Slough, Berkshire, UKCorrespondence: Oluwajoba AdegokeReckitt Benckiser Healthcare International Ltd, Slough, Berkshire, UKTel +44 1482 582134Email joba.adegoke@rb.comObjective: Flurbiprofen 8.75 mg lozenge and spray are used for symptomatic relief of sore throat, with a rapid onset of analgesia suggesting a localized mechanism of action. Building on previous studies, this investigation aimed to use microautoradiography to visualize the depth penetration of radiolabeled flurbiprofen into human pharynx tissue using an ex vivo model. Quantification of flurbiprofen in the tissue was performed to provide a quantitative representation of flurbiprofen distribution through the tissue.Methods: Cadaveric human pharynx tissue was mounted between the donor and receiver compartments of a Franz diffusion cell. After that 8.75 mg spray and dissolved lozenge formulations, containing radiolabeled flurbiprofen, were added to the donor compartment of a Franz diffusion cell. After incubation for one hour, the pharynx tissue was removed, processed, and sectioned both horizontally and vertically. The sections were placed within an imaging cassette to determine the penetration of radiolabeled flurbiprofen visually, before being solubilized to quantify the amount of flurbiprofen present in each section.Results: In the horizontally sectioned samples, flurbiprofen was present in the top layers of all replicates and decreased in intensity throughout the tissue. Of the applied dose, 48.0– 99.9% of flurbiprofen was detected in the top one-third of the pharynx tissue, closest to the dosing site, and 0– 14.8% of flurbiprofen was detected within the deepest third of pharynx tissue, furthest from the dosing site. In the vertically sectioned tissue samples, radiolabeled flurbiprofen was found at a high intensity at the dosing site and reduced in intensity throughout the thickness of the tissue. Lateral penetration of flurbiprofen was also seen in tissue dosed with the spray.Conclusion: Our findings demonstrate that lozenge and spray formulations of flurbiprofen can penetrate throughout the layers of cadaveric human pharynx tissue in an ex vivo model, as visualized by microautoradiography.Keywords: flurbiprofen, Franz diffusion cell, microautoradiography, pharynx
format	article
author	Turner R Wevrett SR Edmunds S Brown MB Kulasekaran A Adegoke O Farrah J
author_facet	Turner R Wevrett SR Edmunds S Brown MB Kulasekaran A Adegoke O Farrah J
author_sort	Turner R
title	Penetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method
title_short	Penetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method
title_full	Penetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method
title_fullStr	Penetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method
title_full_unstemmed	Penetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method
title_sort	penetration of flurbiprofen from a locally applied sore throat lozenge and spray into cadaveric human pharynx tissue: a novel ex vivo model and microautoradiography method
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/5a3cb85d136d4f6895ff4346fa20b3ba
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Penetration of Flurbiprofen from a Locally Applied Sore Throat Lozenge and Spray into Cadaveric Human Pharynx Tissue: A Novel ex vivo Model and Microautoradiography Method

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