Deletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.

<h4>Background</h4>The integron is a genetic recombination system that catalyses the acquisition of genes on mobilisable elements called gene cassettes. In Vibrio species, multiple acquired gene cassettes form a cassette array that can comprise 1-3% of the bacterial genome. Since 75% of...

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Autores principales:	Rita A Rapa, Ronald Shimmon, Steven P Djordjevic, H W Stokes, Maurizio Labbate
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a43047722f84f9bb9c5e3b0852f4f44
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spelling	oai:doaj.org-article:5a43047722f84f9bb9c5e3b0852f4f442021-11-18T07:54:32ZDeletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.1932-620310.1371/journal.pone.0058430https://doaj.org/article/5a43047722f84f9bb9c5e3b0852f4f442013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23484028/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The integron is a genetic recombination system that catalyses the acquisition of genes on mobilisable elements called gene cassettes. In Vibrio species, multiple acquired gene cassettes form a cassette array that can comprise 1-3% of the bacterial genome. Since 75% of these gene cassettes contain genes encoding proteins of uncharacterised function, how the integron has driven adaptation and evolution in Vibrio species remains largely unknown. A feature of cassette arrays is the presence of large indels. Using Vibrio rotiferianus DAT722 as a model organism, the aim of this study was to determine how large cassette deletions affect vibrio physiology with a view to improving understanding into how cassette arrays influence bacterial host adaptation and evolution.<h4>Methodology/principal findings</h4>Biological assays and proteomic techniques were utilised to determine how artificially engineered deletions in the cassette array of V. rotiferianus DAT722 affected cell physiology. Multiple phenotypes were identified including changes to growth and expression of outer membrane porins/proteins and metabolic proteins. Furthermore, the deletions altered cell surface polysaccharide with Proton Nuclear Magnetic Resonance on whole cell polysaccharide identifying changes in the carbohydrate ring proton region indicating that gene cassette products may decorate host cell polysaccharide via the addition or removal of functional groups.<h4>Conclusions/significance</h4>From this study, it was concluded that deletion of gene cassettes had a subtle effect on bacterial metabolism but altered host surface polysaccharide. Deletion (and most likely rearrangement and acquisition) of gene cassettes may provide the bacterium with a mechanism to alter its surface properties, thus impacting on phenotypes such as biofilm formation. Biofilm formation was shown to be altered in one of the deletion mutants used in this study. Reworking surface properties may provide an advantage to the bacterium's interactions with organisms such as bacteriophage, protozoan grazers or crustaceans.Rita A RapaRonald ShimmonSteven P DjordjevicH W StokesMaurizio LabbatePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58430 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rita A Rapa Ronald Shimmon Steven P Djordjevic H W Stokes Maurizio Labbate Deletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.
description	<h4>Background</h4>The integron is a genetic recombination system that catalyses the acquisition of genes on mobilisable elements called gene cassettes. In Vibrio species, multiple acquired gene cassettes form a cassette array that can comprise 1-3% of the bacterial genome. Since 75% of these gene cassettes contain genes encoding proteins of uncharacterised function, how the integron has driven adaptation and evolution in Vibrio species remains largely unknown. A feature of cassette arrays is the presence of large indels. Using Vibrio rotiferianus DAT722 as a model organism, the aim of this study was to determine how large cassette deletions affect vibrio physiology with a view to improving understanding into how cassette arrays influence bacterial host adaptation and evolution.<h4>Methodology/principal findings</h4>Biological assays and proteomic techniques were utilised to determine how artificially engineered deletions in the cassette array of V. rotiferianus DAT722 affected cell physiology. Multiple phenotypes were identified including changes to growth and expression of outer membrane porins/proteins and metabolic proteins. Furthermore, the deletions altered cell surface polysaccharide with Proton Nuclear Magnetic Resonance on whole cell polysaccharide identifying changes in the carbohydrate ring proton region indicating that gene cassette products may decorate host cell polysaccharide via the addition or removal of functional groups.<h4>Conclusions/significance</h4>From this study, it was concluded that deletion of gene cassettes had a subtle effect on bacterial metabolism but altered host surface polysaccharide. Deletion (and most likely rearrangement and acquisition) of gene cassettes may provide the bacterium with a mechanism to alter its surface properties, thus impacting on phenotypes such as biofilm formation. Biofilm formation was shown to be altered in one of the deletion mutants used in this study. Reworking surface properties may provide an advantage to the bacterium's interactions with organisms such as bacteriophage, protozoan grazers or crustaceans.
format	article
author	Rita A Rapa Ronald Shimmon Steven P Djordjevic H W Stokes Maurizio Labbate
author_facet	Rita A Rapa Ronald Shimmon Steven P Djordjevic H W Stokes Maurizio Labbate
author_sort	Rita A Rapa
title	Deletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.
title_short	Deletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.
title_full	Deletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.
title_fullStr	Deletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.
title_full_unstemmed	Deletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.
title_sort	deletion of integron-associated gene cassettes impact on the surface properties of vibrio rotiferianus dat722.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5a43047722f84f9bb9c5e3b0852f4f44
work_keys_str_mv	AT ritaarapa deletionofintegronassociatedgenecassettesimpactonthesurfacepropertiesofvibriorotiferianusdat722 AT ronaldshimmon deletionofintegronassociatedgenecassettesimpactonthesurfacepropertiesofvibriorotiferianusdat722 AT stevenpdjordjevic deletionofintegronassociatedgenecassettesimpactonthesurfacepropertiesofvibriorotiferianusdat722 AT hwstokes deletionofintegronassociatedgenecassettesimpactonthesurfacepropertiesofvibriorotiferianusdat722 AT mauriziolabbate deletionofintegronassociatedgenecassettesimpactonthesurfacepropertiesofvibriorotiferianusdat722
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Deletion of integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722.

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