Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.

The β2-adrenergic receptor has been shown to be involved in neuroendocrine differentiation and to contribute to the development of aggressive prostate cancer. In this study we have investigated whether miR-196a plays a role in the regulation of the β2-adrenergic receptor in the LNCaP prostate cancer...

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Autores principales:	Ilaria Guerriero, Håkon Ramberg, Krizia Sagini, Manuel Ramirez-Garrastacho, Kristin A Taskén, Alicia Llorente
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a48ebcc0d1c420c8839fb8af8f5c117
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spelling	oai:doaj.org-article:5a48ebcc0d1c420c8839fb8af8f5c1172021-12-02T20:15:42ZImplication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.1932-620310.1371/journal.pone.0253828https://doaj.org/article/5a48ebcc0d1c420c8839fb8af8f5c1172021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253828https://doaj.org/toc/1932-6203The β2-adrenergic receptor has been shown to be involved in neuroendocrine differentiation and to contribute to the development of aggressive prostate cancer. In this study we have investigated whether miR-196a plays a role in the regulation of the β2-adrenergic receptor in the LNCaP prostate cancer cell line. Our results show that the expression of miR-196a is elevated in LNCaP prostate cancer cells with reduced levels of β2-adrenergic receptor after stably transfection with three different shRNAs. Furthermore, treatment with β-blockers showed that this upregulation is strictly related to the low levels of β2-adrenergic receptor and not to the inhibition of the receptor signaling activity. Finally, we found that the reduced ability of LNCaP cells with low levels of β2-adrenergic receptor to initiate neuroendocrine differentiation under androgen depletion conditions is mediated by miR-196a. In conclusion, this study provides the rational for a role of miR-196a in the β2-adrenergic receptor mediated neuroendocrine differentiation of LNCaP prostate cancer cells.Ilaria GuerrieroHåkon RambergKrizia SaginiManuel Ramirez-GarrastachoKristin A TaskénAlicia LlorentePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253828 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ilaria Guerriero Håkon Ramberg Krizia Sagini Manuel Ramirez-Garrastacho Kristin A Taskén Alicia Llorente Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.
description	The β2-adrenergic receptor has been shown to be involved in neuroendocrine differentiation and to contribute to the development of aggressive prostate cancer. In this study we have investigated whether miR-196a plays a role in the regulation of the β2-adrenergic receptor in the LNCaP prostate cancer cell line. Our results show that the expression of miR-196a is elevated in LNCaP prostate cancer cells with reduced levels of β2-adrenergic receptor after stably transfection with three different shRNAs. Furthermore, treatment with β-blockers showed that this upregulation is strictly related to the low levels of β2-adrenergic receptor and not to the inhibition of the receptor signaling activity. Finally, we found that the reduced ability of LNCaP cells with low levels of β2-adrenergic receptor to initiate neuroendocrine differentiation under androgen depletion conditions is mediated by miR-196a. In conclusion, this study provides the rational for a role of miR-196a in the β2-adrenergic receptor mediated neuroendocrine differentiation of LNCaP prostate cancer cells.
format	article
author	Ilaria Guerriero Håkon Ramberg Krizia Sagini Manuel Ramirez-Garrastacho Kristin A Taskén Alicia Llorente
author_facet	Ilaria Guerriero Håkon Ramberg Krizia Sagini Manuel Ramirez-Garrastacho Kristin A Taskén Alicia Llorente
author_sort	Ilaria Guerriero
title	Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.
title_short	Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.
title_full	Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.
title_fullStr	Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.
title_full_unstemmed	Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.
title_sort	implication of β2-adrenergic receptor and mir-196a correlation in neurite outgrowth of lncap prostate cancer cells.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5a48ebcc0d1c420c8839fb8af8f5c117
work_keys_str_mv	AT ilariaguerriero implicationofb2adrenergicreceptorandmir196acorrelationinneuriteoutgrowthoflncapprostatecancercells AT hakonramberg implicationofb2adrenergicreceptorandmir196acorrelationinneuriteoutgrowthoflncapprostatecancercells AT kriziasagini implicationofb2adrenergicreceptorandmir196acorrelationinneuriteoutgrowthoflncapprostatecancercells AT manuelramirezgarrastacho implicationofb2adrenergicreceptorandmir196acorrelationinneuriteoutgrowthoflncapprostatecancercells AT kristinatasken implicationofb2adrenergicreceptorandmir196acorrelationinneuriteoutgrowthoflncapprostatecancercells AT aliciallorente implicationofb2adrenergicreceptorandmir196acorrelationinneuriteoutgrowthoflncapprostatecancercells
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Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells.

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