Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>

ABSTRACT Superresolution imaging has revealed subcellular structures and protein interactions in many organisms. However, superresolution microscopy with lateral resolution better than 100 nm has not been achieved in photosynthetic cells due to the interference of a high-autofluorescence background....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Riyue Liu, Yaxin Liu, Shichang Liu, Ying Wang, Kim Li, Ning Li, Daiying Xu, Qinglu Zeng
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	FtsZ ring Prochlorococcus cell division cyanobacteria superresolution imaging Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5a49cc2d605d40f4a3f844688cdf22af
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5a49cc2d605d40f4a3f844688cdf22af
record_format	dspace
spelling	oai:doaj.org-article:5a49cc2d605d40f4a3f844688cdf22af2021-11-15T15:51:56ZThree-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>10.1128/mBio.00657-172150-7511https://doaj.org/article/5a49cc2d605d40f4a3f844688cdf22af2017-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00657-17https://doaj.org/toc/2150-7511ABSTRACT Superresolution imaging has revealed subcellular structures and protein interactions in many organisms. However, superresolution microscopy with lateral resolution better than 100 nm has not been achieved in photosynthetic cells due to the interference of a high-autofluorescence background. Here, we developed a photobleaching method to effectively reduce the autofluorescence of cyanobacterial and plant cells. We achieved lateral resolution of ~10 nm with stochastic optical reconstruction microscopy (STORM) in the sphere-shaped cyanobacterium Prochlorococcus and the flowering plant Arabidopsis thaliana. During the cell cycle of Prochlorococcus, we characterized the three-dimensional (3D) organization of the cell division protein FtsZ, which forms a ring structure at the division site and is important for cytokinesis of bacteria and chloroplasts. Although the FtsZ ring assembly process in rod-shaped bacteria has been studied extensively, it has rarely been studied in sphere-shaped bacteria. Similarly to rod-shaped bacteria, our results with Prochlorococcus also showed the assembly of FtsZ clusters into incomplete rings and then complete rings during cell division. Differently from rod-shaped bacteria, the FtsZ ring diameter was not found to decrease during Prochlorococcus cell division. We also discovered a novel double-Z-ring structure, which may be the Z rings of two daughter cells in a predivisional mother cell. Our results showed a quantitative picture of the in vivo Z ring organization of sphere-shaped bacteria. IMPORTANCE Superresolution microscopy has not been widely used to study photosynthetic cells due to their high-autofluorescence background. Here, we developed a photobleaching method to reduce the autofluorescence of cyanobacteria and plant cells. After photobleaching, we performed superresolution imaging in the cyanobacterium Prochlorococcus and the flowering plant Arabidopsis thaliana with ~10-nm resolution, which is the highest resolution in a photosynthetic cell. With this method, we characterized the 3D organization of the cell division protein FtsZ in Prochlorococcus. We found that the morphological variation of the FtsZ ring during cell division of the sphere-shaped cyanobacterium Prochlorococcus is similar but not identical to that of rod-shaped bacteria. Our method might also be applicable to other photosynthetic organisms.Riyue LiuYaxin LiuShichang LiuYing WangKim LiNing LiDaiying XuQinglu ZengAmerican Society for MicrobiologyarticleFtsZ ringProchlorococcuscell divisioncyanobacteriasuperresolution imagingMicrobiologyQR1-502ENmBio, Vol 8, Iss 6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	FtsZ ring Prochlorococcus cell division cyanobacteria superresolution imaging Microbiology QR1-502
spellingShingle	FtsZ ring Prochlorococcus cell division cyanobacteria superresolution imaging Microbiology QR1-502 Riyue Liu Yaxin Liu Shichang Liu Ying Wang Kim Li Ning Li Daiying Xu Qinglu Zeng Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>
description	ABSTRACT Superresolution imaging has revealed subcellular structures and protein interactions in many organisms. However, superresolution microscopy with lateral resolution better than 100 nm has not been achieved in photosynthetic cells due to the interference of a high-autofluorescence background. Here, we developed a photobleaching method to effectively reduce the autofluorescence of cyanobacterial and plant cells. We achieved lateral resolution of ~10 nm with stochastic optical reconstruction microscopy (STORM) in the sphere-shaped cyanobacterium Prochlorococcus and the flowering plant Arabidopsis thaliana. During the cell cycle of Prochlorococcus, we characterized the three-dimensional (3D) organization of the cell division protein FtsZ, which forms a ring structure at the division site and is important for cytokinesis of bacteria and chloroplasts. Although the FtsZ ring assembly process in rod-shaped bacteria has been studied extensively, it has rarely been studied in sphere-shaped bacteria. Similarly to rod-shaped bacteria, our results with Prochlorococcus also showed the assembly of FtsZ clusters into incomplete rings and then complete rings during cell division. Differently from rod-shaped bacteria, the FtsZ ring diameter was not found to decrease during Prochlorococcus cell division. We also discovered a novel double-Z-ring structure, which may be the Z rings of two daughter cells in a predivisional mother cell. Our results showed a quantitative picture of the in vivo Z ring organization of sphere-shaped bacteria. IMPORTANCE Superresolution microscopy has not been widely used to study photosynthetic cells due to their high-autofluorescence background. Here, we developed a photobleaching method to reduce the autofluorescence of cyanobacteria and plant cells. After photobleaching, we performed superresolution imaging in the cyanobacterium Prochlorococcus and the flowering plant Arabidopsis thaliana with ~10-nm resolution, which is the highest resolution in a photosynthetic cell. With this method, we characterized the 3D organization of the cell division protein FtsZ in Prochlorococcus. We found that the morphological variation of the FtsZ ring during cell division of the sphere-shaped cyanobacterium Prochlorococcus is similar but not identical to that of rod-shaped bacteria. Our method might also be applicable to other photosynthetic organisms.
format	article
author	Riyue Liu Yaxin Liu Shichang Liu Ying Wang Kim Li Ning Li Daiying Xu Qinglu Zeng
author_facet	Riyue Liu Yaxin Liu Shichang Liu Ying Wang Kim Li Ning Li Daiying Xu Qinglu Zeng
author_sort	Riyue Liu
title	Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>
title_short	Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>
title_full	Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>
title_fullStr	Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>
title_full_unstemmed	Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>
title_sort	three-dimensional superresolution imaging of the ftsz ring during cell division of the cyanobacterium <italic toggle="yes">prochlorococcus</italic>
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/5a49cc2d605d40f4a3f844688cdf22af
work_keys_str_mv	AT riyueliu threedimensionalsuperresolutionimagingoftheftszringduringcelldivisionofthecyanobacteriumitalictoggleyesprochlorococcusitalic AT yaxinliu threedimensionalsuperresolutionimagingoftheftszringduringcelldivisionofthecyanobacteriumitalictoggleyesprochlorococcusitalic AT shichangliu threedimensionalsuperresolutionimagingoftheftszringduringcelldivisionofthecyanobacteriumitalictoggleyesprochlorococcusitalic AT yingwang threedimensionalsuperresolutionimagingoftheftszringduringcelldivisionofthecyanobacteriumitalictoggleyesprochlorococcusitalic AT kimli threedimensionalsuperresolutionimagingoftheftszringduringcelldivisionofthecyanobacteriumitalictoggleyesprochlorococcusitalic AT ningli threedimensionalsuperresolutionimagingoftheftszringduringcelldivisionofthecyanobacteriumitalictoggleyesprochlorococcusitalic AT daiyingxu threedimensionalsuperresolutionimagingoftheftszringduringcelldivisionofthecyanobacteriumitalictoggleyesprochlorococcusitalic AT qingluzeng threedimensionalsuperresolutionimagingoftheftszringduringcelldivisionofthecyanobacteriumitalictoggleyesprochlorococcusitalic
_version_	1718427277152223232

Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic>

Ejemplares similares