Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation

Prevalence and incidence of the marine toxins (paralytic, amnesic, and lipophilic toxins) including the so-called emerging toxins (these are, gymnodimines, pinnatoxins, or spirolides among others) have increased in recent years all over the world. Climate change, which is affecting the distribution...

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Autores principales:	Araceli E. Rossignoli, Carmen Mariño, Helena Martín, Juan Blanco
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	emerging toxins fast method LC-MS/MS lipophilic toxin performance screening Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5a4a87122cbd4001bef7fe17f4ec30a4
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spelling	oai:doaj.org-article:5a4a87122cbd4001bef7fe17f4ec30a42021-11-25T18:12:45ZDevelopment of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation10.3390/md191106031660-3397https://doaj.org/article/5a4a87122cbd4001bef7fe17f4ec30a42021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/603https://doaj.org/toc/1660-3397Prevalence and incidence of the marine toxins (paralytic, amnesic, and lipophilic toxins) including the so-called emerging toxins (these are, gymnodimines, pinnatoxins, or spirolides among others) have increased in recent years all over the world. Climate change, which is affecting the distribution of their producing phytoplankton species, is probably one of the main causes. Early detection of the toxins present in a particular area, and linking the toxins to their causative phytoplankton species are key tools to minimize the risk they pose for human consumers. The development of both types of studies requires fast and highly sensitive analytical methods. In the present work, we have developed a highly sensitive liquid chromatography-mass spectrometry methodology (LC-MS/MS), using a column with fused-core particle technology, for the determination of fourteen lipophilic toxins in a single run of 3.6 min. The performance of the method was evaluated for specificity, linearity, precision (repeatability and reproducibility) and accuracy by analysing spiked and naturally contaminated samples. The in-house validation was successful, and the limit of detection (LOD) and quantification (LOQ) for all the toxins were far below their regulatory action limits. The method is suitable to be considered in monitoring systems of bivalves for food control.Araceli E. RossignoliCarmen MariñoHelena MartínJuan BlancoMDPI AGarticleemerging toxinsfast methodLC-MS/MSlipophilic toxinperformancescreeningBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 603, p 603 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	emerging toxins fast method LC-MS/MS lipophilic toxin performance screening Biology (General) QH301-705.5
spellingShingle	emerging toxins fast method LC-MS/MS lipophilic toxin performance screening Biology (General) QH301-705.5 Araceli E. Rossignoli Carmen Mariño Helena Martín Juan Blanco Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation
description	Prevalence and incidence of the marine toxins (paralytic, amnesic, and lipophilic toxins) including the so-called emerging toxins (these are, gymnodimines, pinnatoxins, or spirolides among others) have increased in recent years all over the world. Climate change, which is affecting the distribution of their producing phytoplankton species, is probably one of the main causes. Early detection of the toxins present in a particular area, and linking the toxins to their causative phytoplankton species are key tools to minimize the risk they pose for human consumers. The development of both types of studies requires fast and highly sensitive analytical methods. In the present work, we have developed a highly sensitive liquid chromatography-mass spectrometry methodology (LC-MS/MS), using a column with fused-core particle technology, for the determination of fourteen lipophilic toxins in a single run of 3.6 min. The performance of the method was evaluated for specificity, linearity, precision (repeatability and reproducibility) and accuracy by analysing spiked and naturally contaminated samples. The in-house validation was successful, and the limit of detection (LOD) and quantification (LOQ) for all the toxins were far below their regulatory action limits. The method is suitable to be considered in monitoring systems of bivalves for food control.
format	article
author	Araceli E. Rossignoli Carmen Mariño Helena Martín Juan Blanco
author_facet	Araceli E. Rossignoli Carmen Mariño Helena Martín Juan Blanco
author_sort	Araceli E. Rossignoli
title	Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation
title_short	Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation
title_full	Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation
title_fullStr	Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation
title_full_unstemmed	Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation
title_sort	development of a fast liquid chromatography coupled to mass spectrometry method (lc-ms/ms) to determine fourteen lipophilic shellfish toxins based on fused–core technology: in-house validation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5a4a87122cbd4001bef7fe17f4ec30a4
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Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused–Core Technology: In-House Validation

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