The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp

Abstract Natural competency requires uptake of exogenous DNA from the environment and the integration of that DNA into recipient bacteria can be used for DNA-repair or genetic diversification. The Burkholderia genus is unique in that only some of the species and strains are naturally competent. We i...

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Autores principales:	Yun Heacock-Kang, Ian A. McMillan, Jan Zarzycki-Siek, Zhenxin Sun, Andrew P. Bluhm, Darlene Cabanas, Tung T. Hoang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a535abc1cd140ba895e895a2e73f401
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spelling	oai:doaj.org-article:5a535abc1cd140ba895e895a2e73f4012021-12-02T15:08:35ZThe heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp10.1038/s41598-018-30853-42045-2322https://doaj.org/article/5a535abc1cd140ba895e895a2e73f4012018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30853-4https://doaj.org/toc/2045-2322Abstract Natural competency requires uptake of exogenous DNA from the environment and the integration of that DNA into recipient bacteria can be used for DNA-repair or genetic diversification. The Burkholderia genus is unique in that only some of the species and strains are naturally competent. We identified and characterized two genes, comE and crp, from naturally competent B. pseudomallei 1026b that play a role in DNA uptake and catabolism. Single-copies of rhamnose-inducible comE and crp genes were integrated into a Tn7 attachment-site in non-naturally competent Burkholderia including pathogens B. pseudomallei K96243, B. cenocepacia K56-2, and B. mallei ATCC23344. Strains expressing comE or crp were assayed for their ability to uptake and catabolize DNA. ComE and Crp allowed non-naturally competent Burkholderia species to catabolize DNA, uptake exogenous gfp DNA and express GFP. Furthermore, we used synthetic comE and crp to expand the utility of the λ-red recombineering system for genetic manipulation of non-competent Burkholderia species. A newly constructed vector, pKaKa4, was used to mutate the aspartate semialdehyde dehydrogenase (asd) gene in four B. mallei strains, leading to the complete attenuation of these tier-1 select-agents. These strains have been excluded from select-agent regulations and will be of great interest to the field.Yun Heacock-KangIan A. McMillanJan Zarzycki-SiekZhenxin SunAndrew P. BluhmDarlene CabanasTung T. HoangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yun Heacock-Kang Ian A. McMillan Jan Zarzycki-Siek Zhenxin Sun Andrew P. Bluhm Darlene Cabanas Tung T. Hoang The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp
description	Abstract Natural competency requires uptake of exogenous DNA from the environment and the integration of that DNA into recipient bacteria can be used for DNA-repair or genetic diversification. The Burkholderia genus is unique in that only some of the species and strains are naturally competent. We identified and characterized two genes, comE and crp, from naturally competent B. pseudomallei 1026b that play a role in DNA uptake and catabolism. Single-copies of rhamnose-inducible comE and crp genes were integrated into a Tn7 attachment-site in non-naturally competent Burkholderia including pathogens B. pseudomallei K96243, B. cenocepacia K56-2, and B. mallei ATCC23344. Strains expressing comE or crp were assayed for their ability to uptake and catabolize DNA. ComE and Crp allowed non-naturally competent Burkholderia species to catabolize DNA, uptake exogenous gfp DNA and express GFP. Furthermore, we used synthetic comE and crp to expand the utility of the λ-red recombineering system for genetic manipulation of non-competent Burkholderia species. A newly constructed vector, pKaKa4, was used to mutate the aspartate semialdehyde dehydrogenase (asd) gene in four B. mallei strains, leading to the complete attenuation of these tier-1 select-agents. These strains have been excluded from select-agent regulations and will be of great interest to the field.
format	article
author	Yun Heacock-Kang Ian A. McMillan Jan Zarzycki-Siek Zhenxin Sun Andrew P. Bluhm Darlene Cabanas Tung T. Hoang
author_facet	Yun Heacock-Kang Ian A. McMillan Jan Zarzycki-Siek Zhenxin Sun Andrew P. Bluhm Darlene Cabanas Tung T. Hoang
author_sort	Yun Heacock-Kang
title	The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp
title_short	The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp
title_full	The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp
title_fullStr	The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp
title_full_unstemmed	The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp
title_sort	heritable natural competency trait of burkholderia pseudomallei in other burkholderia species through come and crp
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5a535abc1cd140ba895e895a2e73f401
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The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp

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