Chemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.

Root and bark of Limonium axillare (Forssk) Kuntze are used as antidiabetic remedies in parts of East Africa, but this activity has never been fully investigated. To validate its ethnobotanical use, we compared the chemical and pharmacological profiles of the ethanolic extracts of L. axillare root (...

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Autores principales:	Essam Abdel-Sattar, Manal M Shams, Marwa M Abd-Rabo, Nehad Mahmoud, Engy A Mahrous
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a579465b4cc49f88fa1d71b1facffba
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spelling	oai:doaj.org-article:5a579465b4cc49f88fa1d71b1facffba2021-12-02T20:18:32ZChemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.1932-620310.1371/journal.pone.0255904https://doaj.org/article/5a579465b4cc49f88fa1d71b1facffba2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255904https://doaj.org/toc/1932-6203Root and bark of Limonium axillare (Forssk) Kuntze are used as antidiabetic remedies in parts of East Africa, but this activity has never been fully investigated. To validate its ethnobotanical use, we compared the chemical and pharmacological profiles of the ethanolic extracts of L. axillare root (REE) and aerial parts (AEE). Administration of REE (500 mg kg-1) reduced streptozotocin-induced hyperglycemia by 44%, restored serum insulin levels, reestablished Glut2 and Glut4 expression and ameliorated pancreatic tissue damage in diabetic rats. In vitro studies revealed a strong radical scavenging effect, α-glucosidase, and α-amylase inhibition activity of REE at IC50 at 25.2, 44.8 and 89.1μg/mL, respectively. HPLC analysis identified ten phenolic compounds in REE with umbelliferone as the major constituents at 10 ± 0.081 mg/g of extract. Additionally, six compounds were isolated from REE including, β-sitosterol-3-palmitate, β-sitosterol, myricetin and gallic acids with two new tetrahydrofuran monoterpenes; 2-isopropyl- 3,4,4, trimethyl-tetrahydrofuran (3), and 2-isopropyl-4-methyl-tetrahydrofuran-3,4 dicarboxylic acid (4), the latter was revealed by molecular docking to be a good ligand to glycerol-3-phosphate dehydrogenase a key enzyme in glycolysis.Essam Abdel-SattarManal M ShamsMarwa M Abd-RaboNehad MahmoudEngy A MahrousPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255904 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Essam Abdel-Sattar Manal M Shams Marwa M Abd-Rabo Nehad Mahmoud Engy A Mahrous Chemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.
description	Root and bark of Limonium axillare (Forssk) Kuntze are used as antidiabetic remedies in parts of East Africa, but this activity has never been fully investigated. To validate its ethnobotanical use, we compared the chemical and pharmacological profiles of the ethanolic extracts of L. axillare root (REE) and aerial parts (AEE). Administration of REE (500 mg kg-1) reduced streptozotocin-induced hyperglycemia by 44%, restored serum insulin levels, reestablished Glut2 and Glut4 expression and ameliorated pancreatic tissue damage in diabetic rats. In vitro studies revealed a strong radical scavenging effect, α-glucosidase, and α-amylase inhibition activity of REE at IC50 at 25.2, 44.8 and 89.1μg/mL, respectively. HPLC analysis identified ten phenolic compounds in REE with umbelliferone as the major constituents at 10 ± 0.081 mg/g of extract. Additionally, six compounds were isolated from REE including, β-sitosterol-3-palmitate, β-sitosterol, myricetin and gallic acids with two new tetrahydrofuran monoterpenes; 2-isopropyl- 3,4,4, trimethyl-tetrahydrofuran (3), and 2-isopropyl-4-methyl-tetrahydrofuran-3,4 dicarboxylic acid (4), the latter was revealed by molecular docking to be a good ligand to glycerol-3-phosphate dehydrogenase a key enzyme in glycolysis.
format	article
author	Essam Abdel-Sattar Manal M Shams Marwa M Abd-Rabo Nehad Mahmoud Engy A Mahrous
author_facet	Essam Abdel-Sattar Manal M Shams Marwa M Abd-Rabo Nehad Mahmoud Engy A Mahrous
author_sort	Essam Abdel-Sattar
title	Chemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.
title_short	Chemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.
title_full	Chemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.
title_fullStr	Chemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.
title_full_unstemmed	Chemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.
title_sort	chemical and biological investigations of limonium axillare reveal mechanistic evidence for its antidiabetic activity.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5a579465b4cc49f88fa1d71b1facffba
work_keys_str_mv	AT essamabdelsattar chemicalandbiologicalinvestigationsoflimoniumaxillarerevealmechanisticevidenceforitsantidiabeticactivity AT manalmshams chemicalandbiologicalinvestigationsoflimoniumaxillarerevealmechanisticevidenceforitsantidiabeticactivity AT marwamabdrabo chemicalandbiologicalinvestigationsoflimoniumaxillarerevealmechanisticevidenceforitsantidiabeticactivity AT nehadmahmoud chemicalandbiologicalinvestigationsoflimoniumaxillarerevealmechanisticevidenceforitsantidiabeticactivity AT engyamahrous chemicalandbiologicalinvestigationsoflimoniumaxillarerevealmechanisticevidenceforitsantidiabeticactivity
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Chemical and biological investigations of Limonium axillare reveal mechanistic evidence for its antidiabetic activity.

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