An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1

Abstract Crops expressing Bacillus thuringiensis (Bt)-derived insecticidal protein genes have been commercially available for over 15 years and are providing significant value to growers. However, there remains the need for alternative insecticidal actives due to emerging insect resistance to certai...

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Autores principales:	Nasser Yalpani, Dan Altier, Jennifer Barry, Adane Kassa, Timothy M. Nowatzki, Amit Sethi, Jian-Zhou Zhao, Scott Diehn, Virginia Crane, Gary Sandahl, Rongjin Guan, Brad Poland, Claudia Perez Ortega, Mark E. Nelson, Weiping Xie, Lu Liu, Gusui Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a594af16a0d4915b7b36b5136fb768b
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spelling	oai:doaj.org-article:5a594af16a0d4915b7b36b5136fb768b2021-12-02T16:06:19ZAn Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab110.1038/s41598-017-03544-92045-2322https://doaj.org/article/5a594af16a0d4915b7b36b5136fb768b2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03544-9https://doaj.org/toc/2045-2322Abstract Crops expressing Bacillus thuringiensis (Bt)-derived insecticidal protein genes have been commercially available for over 15 years and are providing significant value to growers. However, there remains the need for alternative insecticidal actives due to emerging insect resistance to certain Bt proteins. A screen of bacterial strains led to the discovery of a two-component insecticidal protein named AfIP-1A/1B from an Alcaligenes faecalis strain. This protein shows selectivity against coleopteran insects including western corn rootworm (WCR). Transgenic maize plants expressing AfIP-1A/1B demonstrate strong protection from rootworm injury. Surprisingly, although little sequence similarity exists to known insecticidal proteins, efficacy tests using WCR populations resistant to two different Cry proteins show that AfIP-1A/1B and mCry3A differ in their mode of action while AfIP-1A/1B and the binary Cry34Ab1/Cry35Ab1 protein share a similar mode. These findings are supported by results of competitive binding assays and the similarity of the x-ray structure of AfIP-1A to Cry34Ab1. Our work indicates that insecticidal proteins obtained from a non-Bt bacterial source can be useful for developing genetically modified crops and can function similarly to familiar proteins from Bt.Nasser YalpaniDan AltierJennifer BarryAdane KassaTimothy M. NowatzkiAmit SethiJian-Zhou ZhaoScott DiehnVirginia CraneGary SandahlRongjin GuanBrad PolandClaudia Perez OrtegaMark E. NelsonWeiping XieLu LiuGusui WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nasser Yalpani Dan Altier Jennifer Barry Adane Kassa Timothy M. Nowatzki Amit Sethi Jian-Zhou Zhao Scott Diehn Virginia Crane Gary Sandahl Rongjin Guan Brad Poland Claudia Perez Ortega Mark E. Nelson Weiping Xie Lu Liu Gusui Wu An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1
description	Abstract Crops expressing Bacillus thuringiensis (Bt)-derived insecticidal protein genes have been commercially available for over 15 years and are providing significant value to growers. However, there remains the need for alternative insecticidal actives due to emerging insect resistance to certain Bt proteins. A screen of bacterial strains led to the discovery of a two-component insecticidal protein named AfIP-1A/1B from an Alcaligenes faecalis strain. This protein shows selectivity against coleopteran insects including western corn rootworm (WCR). Transgenic maize plants expressing AfIP-1A/1B demonstrate strong protection from rootworm injury. Surprisingly, although little sequence similarity exists to known insecticidal proteins, efficacy tests using WCR populations resistant to two different Cry proteins show that AfIP-1A/1B and mCry3A differ in their mode of action while AfIP-1A/1B and the binary Cry34Ab1/Cry35Ab1 protein share a similar mode. These findings are supported by results of competitive binding assays and the similarity of the x-ray structure of AfIP-1A to Cry34Ab1. Our work indicates that insecticidal proteins obtained from a non-Bt bacterial source can be useful for developing genetically modified crops and can function similarly to familiar proteins from Bt.
format	article
author	Nasser Yalpani Dan Altier Jennifer Barry Adane Kassa Timothy M. Nowatzki Amit Sethi Jian-Zhou Zhao Scott Diehn Virginia Crane Gary Sandahl Rongjin Guan Brad Poland Claudia Perez Ortega Mark E. Nelson Weiping Xie Lu Liu Gusui Wu
author_facet	Nasser Yalpani Dan Altier Jennifer Barry Adane Kassa Timothy M. Nowatzki Amit Sethi Jian-Zhou Zhao Scott Diehn Virginia Crane Gary Sandahl Rongjin Guan Brad Poland Claudia Perez Ortega Mark E. Nelson Weiping Xie Lu Liu Gusui Wu
author_sort	Nasser Yalpani
title	An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1
title_short	An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1
title_full	An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1
title_fullStr	An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1
title_full_unstemmed	An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1
title_sort	alcaligenes strain emulates bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to cry34ab1/cry35ab1
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5a594af16a0d4915b7b36b5136fb768b
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An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1

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