Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice

Visceral obesity may be a driving factor in nonalcoholic fatty liver disease (NAFLD) development. Previous studies have shown that the omega-3 polyunsaturated fatty acid, eicosapentaenoic acid (EPA), ameliorates obesity in high-fat (HF) fed male, C57Bl/6 mice at thermoneutral conditions, independent...

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Autores principales:	Kembra Albracht-Schulte, Savanna Wilson, Paige Johnson, Mandana Pahlavani, Latha Ramalingam, Bimba Goonapienuwala, Nishan S. Kalupahana, William T. Festuccia, Shane Scoggin, Chanaka N. Kahathuduwa, Naima Moustaid-Moussa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	eicosapentaenoic acid (EPA) nonalcoholic fatty liver disease (NAFLD) obesity omega-3 polyunsaturated fatty acids thermoneutrality uncoupling protein 1 (UCP1) Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5a594c163c1947aa81b9e97d8ce04d55
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spelling	oai:doaj.org-article:5a594c163c1947aa81b9e97d8ce04d552021-11-25T16:48:54ZSex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice10.3390/biomedicines91115492227-9059https://doaj.org/article/5a594c163c1947aa81b9e97d8ce04d552021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1549https://doaj.org/toc/2227-9059Visceral obesity may be a driving factor in nonalcoholic fatty liver disease (NAFLD) development. Previous studies have shown that the omega-3 polyunsaturated fatty acid, eicosapentaenoic acid (EPA), ameliorates obesity in high-fat (HF) fed male, C57Bl/6 mice at thermoneutral conditions, independent of uncoupling protein 1 (UCP1). Our goals herein were to investigate sex-dependent mechanisms of EPA in the livers of wild type (WT) and UCP1 knockout (KO) male and female mice fed a HF diet (45% kcal fat; WT-HF, KO-HF) with or without supplementation of 36 g/kg EPA (WT-EPA, KO-EPA). KO significantly increased body weight in males, with no significant reductions with EPA in the WT or KO groups. In females, there were no significant differences in body weight among KO groups and no effects of EPA. In males, liver TGs were significantly higher in the KO-HF group and reduced with EPA, which was not observed in females. Accordingly, gene and protein markers of mitochondrial oxidation, peroxisomal biogenesis and oxidation, as well as metabolic futile cycles were sex-dependently impacted by KO and EPA supplementation. These findings suggest a genotypic difference in response to dietary EPA supplementation on the livers of male and female mice with diet-induced obesity and housed at thermoneutrality.Kembra Albracht-SchulteSavanna WilsonPaige JohnsonMandana PahlavaniLatha RamalingamBimba GoonapienuwalaNishan S. KalupahanaWilliam T. FestucciaShane ScogginChanaka N. KahathuduwaNaima Moustaid-MoussaMDPI AGarticleeicosapentaenoic acid (EPA)nonalcoholic fatty liver disease (NAFLD)obesityomega-3 polyunsaturated fatty acidsthermoneutralityuncoupling protein 1 (UCP1)Biology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1549, p 1549 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	eicosapentaenoic acid (EPA) nonalcoholic fatty liver disease (NAFLD) obesity omega-3 polyunsaturated fatty acids thermoneutrality uncoupling protein 1 (UCP1) Biology (General) QH301-705.5
spellingShingle	eicosapentaenoic acid (EPA) nonalcoholic fatty liver disease (NAFLD) obesity omega-3 polyunsaturated fatty acids thermoneutrality uncoupling protein 1 (UCP1) Biology (General) QH301-705.5 Kembra Albracht-Schulte Savanna Wilson Paige Johnson Mandana Pahlavani Latha Ramalingam Bimba Goonapienuwala Nishan S. Kalupahana William T. Festuccia Shane Scoggin Chanaka N. Kahathuduwa Naima Moustaid-Moussa Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice
description	Visceral obesity may be a driving factor in nonalcoholic fatty liver disease (NAFLD) development. Previous studies have shown that the omega-3 polyunsaturated fatty acid, eicosapentaenoic acid (EPA), ameliorates obesity in high-fat (HF) fed male, C57Bl/6 mice at thermoneutral conditions, independent of uncoupling protein 1 (UCP1). Our goals herein were to investigate sex-dependent mechanisms of EPA in the livers of wild type (WT) and UCP1 knockout (KO) male and female mice fed a HF diet (45% kcal fat; WT-HF, KO-HF) with or without supplementation of 36 g/kg EPA (WT-EPA, KO-EPA). KO significantly increased body weight in males, with no significant reductions with EPA in the WT or KO groups. In females, there were no significant differences in body weight among KO groups and no effects of EPA. In males, liver TGs were significantly higher in the KO-HF group and reduced with EPA, which was not observed in females. Accordingly, gene and protein markers of mitochondrial oxidation, peroxisomal biogenesis and oxidation, as well as metabolic futile cycles were sex-dependently impacted by KO and EPA supplementation. These findings suggest a genotypic difference in response to dietary EPA supplementation on the livers of male and female mice with diet-induced obesity and housed at thermoneutrality.
format	article
author	Kembra Albracht-Schulte Savanna Wilson Paige Johnson Mandana Pahlavani Latha Ramalingam Bimba Goonapienuwala Nishan S. Kalupahana William T. Festuccia Shane Scoggin Chanaka N. Kahathuduwa Naima Moustaid-Moussa
author_facet	Kembra Albracht-Schulte Savanna Wilson Paige Johnson Mandana Pahlavani Latha Ramalingam Bimba Goonapienuwala Nishan S. Kalupahana William T. Festuccia Shane Scoggin Chanaka N. Kahathuduwa Naima Moustaid-Moussa
author_sort	Kembra Albracht-Schulte
title	Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice
title_short	Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice
title_full	Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice
title_fullStr	Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice
title_full_unstemmed	Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice
title_sort	sex-dependent effects of eicosapentaenoic acid on hepatic steatosis in ucp1 knockout mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5a594c163c1947aa81b9e97d8ce04d55
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Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice

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