Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers

African swine fever virus (ASFV) causes acute hemorrhagic fever in domestic pigs and wild boars, resulting in incalculable economic losses to the pig industry. As the mechanism of viral infection is not clear, protective antigens have not been discovered or identified. In this study, we determined t...

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Autores principales:	Wenqiang Sun, He Zhang, Wenhui Fan, Lihong He, Teng Chen, Xintao Zhou, Yu Qi, Lei Sun, Rongliang Hu, Tingrong Luo, Wenjun Liu, Jing Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	African swine fever virus protective antigen T epitopes T cell immune response p72 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5a5b512fd96e4fe2903906ad33a29185
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spelling	oai:doaj.org-article:5a5b512fd96e4fe2903906ad33a291852021-11-25T19:13:54ZEvaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers10.3390/v131122641999-4915https://doaj.org/article/5a5b512fd96e4fe2903906ad33a291852021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2264https://doaj.org/toc/1999-4915African swine fever virus (ASFV) causes acute hemorrhagic fever in domestic pigs and wild boars, resulting in incalculable economic losses to the pig industry. As the mechanism of viral infection is not clear, protective antigens have not been discovered or identified. In this study, we determined that the p30, pp62, p72, and CD2v proteins were all involved in the T cell immune response of live pigs infected with ASFV, among which p72 and pp62 proteins were the strongest. Panoramic scanning was performed on T cell epitopes of the p72 protein, and three high-frequency positive epitopes were selected to construct a swine leukocyte antigen (SLA)-tetramer, and ASFV-specific T cells were detected. Subsequently, the specific T cell and humoral immune responses of ASFV-infected pigs and surviving pigs were compared. The results demonstrate that the specific T cellular immunity responses gradually increased during the infection and were higher than that in the surviving pigs in the late stages of infection. The same trend was observed in specific humoral immune responses, which were highest in surviving pigs. In general, our study provides key information for the exploration of ASFV-specific immune responses and the development of an ASFV vaccine.Wenqiang SunHe ZhangWenhui FanLihong HeTeng ChenXintao ZhouYu QiLei SunRongliang HuTingrong LuoWenjun LiuJing LiMDPI AGarticleAfrican swine fever virusprotective antigenT epitopesT cell immune responsep72MicrobiologyQR1-502ENViruses, Vol 13, Iss 2264, p 2264 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	African swine fever virus protective antigen T epitopes T cell immune response p72 Microbiology QR1-502
spellingShingle	African swine fever virus protective antigen T epitopes T cell immune response p72 Microbiology QR1-502 Wenqiang Sun He Zhang Wenhui Fan Lihong He Teng Chen Xintao Zhou Yu Qi Lei Sun Rongliang Hu Tingrong Luo Wenjun Liu Jing Li Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers
description	African swine fever virus (ASFV) causes acute hemorrhagic fever in domestic pigs and wild boars, resulting in incalculable economic losses to the pig industry. As the mechanism of viral infection is not clear, protective antigens have not been discovered or identified. In this study, we determined that the p30, pp62, p72, and CD2v proteins were all involved in the T cell immune response of live pigs infected with ASFV, among which p72 and pp62 proteins were the strongest. Panoramic scanning was performed on T cell epitopes of the p72 protein, and three high-frequency positive epitopes were selected to construct a swine leukocyte antigen (SLA)-tetramer, and ASFV-specific T cells were detected. Subsequently, the specific T cell and humoral immune responses of ASFV-infected pigs and surviving pigs were compared. The results demonstrate that the specific T cellular immunity responses gradually increased during the infection and were higher than that in the surviving pigs in the late stages of infection. The same trend was observed in specific humoral immune responses, which were highest in surviving pigs. In general, our study provides key information for the exploration of ASFV-specific immune responses and the development of an ASFV vaccine.
format	article
author	Wenqiang Sun He Zhang Wenhui Fan Lihong He Teng Chen Xintao Zhou Yu Qi Lei Sun Rongliang Hu Tingrong Luo Wenjun Liu Jing Li
author_facet	Wenqiang Sun He Zhang Wenhui Fan Lihong He Teng Chen Xintao Zhou Yu Qi Lei Sun Rongliang Hu Tingrong Luo Wenjun Liu Jing Li
author_sort	Wenqiang Sun
title	Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers
title_short	Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers
title_full	Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers
title_fullStr	Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers
title_full_unstemmed	Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers
title_sort	evaluation of cellular immunity with asfv infection by swine leukocyte antigen (sla)—peptide tetramers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5a5b512fd96e4fe2903906ad33a29185
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Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers

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