Under-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes

ABSTRACT The incidence of locally acquired dengue infections increased during the last decade in the United States, compelling a sustained research effort concerning the dengue mosquito vector, Aedes aegypti, and its microbiome, which has been shown to influence virus transmission success. We examin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sean M. Boyles, Carla N. Mavian, Esteban Finol, Maria Ukhanova, Caroline J. Stephenson, Gabriela Hamerlinck, Seokyoung Kang, Caleb Baumgartner, Mary Geesey, Israel Stinton, Katie Williams, Derrick K. Mathias, Mattia Prosperi, Volker Mai, Marco Salemi, Eva A. Buckner, John A. Lednicky, Adam R. Rivers, Rhoel R. Dinglasan
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	dengue virus serotype 4 transmission Aedes aegypti DENV4 flavivirus mosquito Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5a5bb94c68b14acd9ffb85935c32f94b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5a5bb94c68b14acd9ffb85935c32f94b
record_format	dspace
spelling	oai:doaj.org-article:5a5bb94c68b14acd9ffb85935c32f94b2021-11-15T15:29:17ZUnder-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes10.1128/mSphere.00316-202379-5042https://doaj.org/article/5a5bb94c68b14acd9ffb85935c32f94b2020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00316-20https://doaj.org/toc/2379-5042ABSTRACT The incidence of locally acquired dengue infections increased during the last decade in the United States, compelling a sustained research effort concerning the dengue mosquito vector, Aedes aegypti, and its microbiome, which has been shown to influence virus transmission success. We examined the “metavirome” of four populations of Aedes aegypti mosquitoes collected in 2016 to 2017 in Manatee County, FL. Unexpectedly, we discovered that dengue virus serotype 4 (DENV4) was circulating in these mosquito populations, representing the first documented case of such a phenomenon in the absence of a local DENV4 human case in this county over a 2-year period. We confirmed that all of the mosquito populations carried the same DENV4 strain, assembled its full genome, validated infection orthogonally by reverse transcriptase PCR, traced the virus origin, estimated the time period of its introduction to the Caribbean region, and explored the viral genetic signatures and mosquito-specific virome associations that potentially mediated DENV4 persistence in mosquitoes. We discuss the significance of prolonged maintenance of the DENV4 infections in A. aegypti that occurred in the absence of a DENV4 human index case in Manatee County with respect to the inability of current surveillance paradigms to detect mosquito vector infections prior to a potential local outbreak. IMPORTANCE Since 1999, dengue outbreaks in the continental United States involving local transmission have occurred only episodically and only in Florida and Texas. In Florida, these episodes appear to be coincident with increased introductions of dengue virus into the region through human travel and migration from countries where the disease is endemic. To date, the U.S. public health response to dengue outbreaks has been largely reactive, and implementation of comprehensive arbovirus surveillance in advance of predictable transmission seasons, which would enable proactive preventative efforts, remains unsupported. The significance of our finding is that it is the first documented report of DENV4 transmission to and maintenance within a local mosquito vector population in the continental United States in the absence of a human case during two consecutive years. Our data suggest that molecular surveillance of mosquito populations in high-risk, high-tourism areas of the United States may enable proactive, targeted vector control before potential arbovirus outbreaks.Sean M. BoylesCarla N. MavianEsteban FinolMaria UkhanovaCaroline J. StephensonGabriela HamerlinckSeokyoung KangCaleb BaumgartnerMary GeeseyIsrael StintonKatie WilliamsDerrick K. MathiasMattia ProsperiVolker MaiMarco SalemiEva A. BucknerJohn A. LednickyAdam R. RiversRhoel R. DinglasanAmerican Society for Microbiologyarticledengue virus serotype 4transmissionAedes aegyptiDENV4flavivirusmosquitoMicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	dengue virus serotype 4 transmission Aedes aegypti DENV4 flavivirus mosquito Microbiology QR1-502
spellingShingle	dengue virus serotype 4 transmission Aedes aegypti DENV4 flavivirus mosquito Microbiology QR1-502 Sean M. Boyles Carla N. Mavian Esteban Finol Maria Ukhanova Caroline J. Stephenson Gabriela Hamerlinck Seokyoung Kang Caleb Baumgartner Mary Geesey Israel Stinton Katie Williams Derrick K. Mathias Mattia Prosperi Volker Mai Marco Salemi Eva A. Buckner John A. Lednicky Adam R. Rivers Rhoel R. Dinglasan Under-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes
description	ABSTRACT The incidence of locally acquired dengue infections increased during the last decade in the United States, compelling a sustained research effort concerning the dengue mosquito vector, Aedes aegypti, and its microbiome, which has been shown to influence virus transmission success. We examined the “metavirome” of four populations of Aedes aegypti mosquitoes collected in 2016 to 2017 in Manatee County, FL. Unexpectedly, we discovered that dengue virus serotype 4 (DENV4) was circulating in these mosquito populations, representing the first documented case of such a phenomenon in the absence of a local DENV4 human case in this county over a 2-year period. We confirmed that all of the mosquito populations carried the same DENV4 strain, assembled its full genome, validated infection orthogonally by reverse transcriptase PCR, traced the virus origin, estimated the time period of its introduction to the Caribbean region, and explored the viral genetic signatures and mosquito-specific virome associations that potentially mediated DENV4 persistence in mosquitoes. We discuss the significance of prolonged maintenance of the DENV4 infections in A. aegypti that occurred in the absence of a DENV4 human index case in Manatee County with respect to the inability of current surveillance paradigms to detect mosquito vector infections prior to a potential local outbreak. IMPORTANCE Since 1999, dengue outbreaks in the continental United States involving local transmission have occurred only episodically and only in Florida and Texas. In Florida, these episodes appear to be coincident with increased introductions of dengue virus into the region through human travel and migration from countries where the disease is endemic. To date, the U.S. public health response to dengue outbreaks has been largely reactive, and implementation of comprehensive arbovirus surveillance in advance of predictable transmission seasons, which would enable proactive preventative efforts, remains unsupported. The significance of our finding is that it is the first documented report of DENV4 transmission to and maintenance within a local mosquito vector population in the continental United States in the absence of a human case during two consecutive years. Our data suggest that molecular surveillance of mosquito populations in high-risk, high-tourism areas of the United States may enable proactive, targeted vector control before potential arbovirus outbreaks.
format	article
author	Sean M. Boyles Carla N. Mavian Esteban Finol Maria Ukhanova Caroline J. Stephenson Gabriela Hamerlinck Seokyoung Kang Caleb Baumgartner Mary Geesey Israel Stinton Katie Williams Derrick K. Mathias Mattia Prosperi Volker Mai Marco Salemi Eva A. Buckner John A. Lednicky Adam R. Rivers Rhoel R. Dinglasan
author_facet	Sean M. Boyles Carla N. Mavian Esteban Finol Maria Ukhanova Caroline J. Stephenson Gabriela Hamerlinck Seokyoung Kang Caleb Baumgartner Mary Geesey Israel Stinton Katie Williams Derrick K. Mathias Mattia Prosperi Volker Mai Marco Salemi Eva A. Buckner John A. Lednicky Adam R. Rivers Rhoel R. Dinglasan
author_sort	Sean M. Boyles
title	Under-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes
title_short	Under-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes
title_full	Under-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes
title_fullStr	Under-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes
title_full_unstemmed	Under-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes
title_sort	under-the-radar dengue virus infections in natural populations of <named-content content-type="genus-species">aedes aegypti</named-content> mosquitoes
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/5a5bb94c68b14acd9ffb85935c32f94b
work_keys_str_mv	AT seanmboyles undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT carlanmavian undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT estebanfinol undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT mariaukhanova undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT carolinejstephenson undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT gabrielahamerlinck undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT seokyoungkang undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT calebbaumgartner undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT marygeesey undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT israelstinton undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT katiewilliams undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT derrickkmathias undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT mattiaprosperi undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT volkermai undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT marcosalemi undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT evaabuckner undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT johnalednicky undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT adamrrivers undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes AT rhoelrdinglasan undertheradardenguevirusinfectionsinnaturalpopulationsofnamedcontentcontenttypegenusspeciesaedesaegyptinamedcontentmosquitoes
_version_	1718427887199059968

Under-the-Radar Dengue Virus Infections in Natural Populations of <named-content content-type="genus-species">Aedes aegypti</named-content> Mosquitoes

Ejemplares similares