Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway

Abstract This study explored the role of fibulin-3 in osteosarcoma progression and the possible signaling pathway involved. Fibulin-3 mRNA and protein expression in normal tissue, benign fibrous dysplasia, osteosarcoma, osteosarcoma cell lines (HOS and U-2OS), the normal osteoblastic cell line hFOB,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Songgang Wang, Dong Zhang, Shasha Han, Peng Gao, Changying Liu, Jianmin Li, Xin Pan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a62527b0fb1473b969f39368b77c320
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5a62527b0fb1473b969f39368b77c320
record_format	dspace
spelling	oai:doaj.org-article:5a62527b0fb1473b969f39368b77c3202021-12-02T16:06:14ZFibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway10.1038/s41598-017-06353-22045-2322https://doaj.org/article/5a62527b0fb1473b969f39368b77c3202017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06353-2https://doaj.org/toc/2045-2322Abstract This study explored the role of fibulin-3 in osteosarcoma progression and the possible signaling pathway involved. Fibulin-3 mRNA and protein expression in normal tissue, benign fibrous dysplasia, osteosarcoma, osteosarcoma cell lines (HOS and U-2OS), the normal osteoblastic cell line hFOB, and different invasive subclones was evaluated by immunohistochemistry (IHC) or immunocytochemistry (ICC) and real time reverse transcriptase-polymerase chain reaction (real time qRT-PCR). To assess the role of fibulin-3 in the invasion and metastasis of osteosarcoma cells, lentiviral vectors with fibulin-3 small hairpin RNA (shRNA) and pLVX-fibulin-3 were constructed and used to infect the highly invasive and low invasive subclones. The effects of fibulin-3 knockdown and upregulation on the biological behavior of osteosarcoma cells were investigated by functional in vitro and in vivo assays. The results revealed that fibulin-3 expression was upregulated in osteosarcoma, and was positively correlated with low differentiation, lymph node metastasis, and poor prognosis. Fibulin-3 could promote osteosarcoma cell invasion and metastasis by inducing EMT and activating the Wnt/β-catenin signaling pathway. Collectively, our findings demonstrate that fibulin-3 is a promoter of osteosarcoma development and progression, and suggest a novel therapeutic target for future studies.Songgang WangDong ZhangShasha HanPeng GaoChangying LiuJianmin LiXin PanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Songgang Wang Dong Zhang Shasha Han Peng Gao Changying Liu Jianmin Li Xin Pan Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway
description	Abstract This study explored the role of fibulin-3 in osteosarcoma progression and the possible signaling pathway involved. Fibulin-3 mRNA and protein expression in normal tissue, benign fibrous dysplasia, osteosarcoma, osteosarcoma cell lines (HOS and U-2OS), the normal osteoblastic cell line hFOB, and different invasive subclones was evaluated by immunohistochemistry (IHC) or immunocytochemistry (ICC) and real time reverse transcriptase-polymerase chain reaction (real time qRT-PCR). To assess the role of fibulin-3 in the invasion and metastasis of osteosarcoma cells, lentiviral vectors with fibulin-3 small hairpin RNA (shRNA) and pLVX-fibulin-3 were constructed and used to infect the highly invasive and low invasive subclones. The effects of fibulin-3 knockdown and upregulation on the biological behavior of osteosarcoma cells were investigated by functional in vitro and in vivo assays. The results revealed that fibulin-3 expression was upregulated in osteosarcoma, and was positively correlated with low differentiation, lymph node metastasis, and poor prognosis. Fibulin-3 could promote osteosarcoma cell invasion and metastasis by inducing EMT and activating the Wnt/β-catenin signaling pathway. Collectively, our findings demonstrate that fibulin-3 is a promoter of osteosarcoma development and progression, and suggest a novel therapeutic target for future studies.
format	article
author	Songgang Wang Dong Zhang Shasha Han Peng Gao Changying Liu Jianmin Li Xin Pan
author_facet	Songgang Wang Dong Zhang Shasha Han Peng Gao Changying Liu Jianmin Li Xin Pan
author_sort	Songgang Wang
title	Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway
title_short	Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway
title_full	Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway
title_fullStr	Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway
title_full_unstemmed	Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway
title_sort	fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the wnt/β-catenin signaling pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5a62527b0fb1473b969f39368b77c320
work_keys_str_mv	AT songgangwang fibulin3promotesosteosarcomainvasionandmetastasisbyinducingepithelialtomesenchymaltransitionandactivatingthewntbcateninsignalingpathway AT dongzhang fibulin3promotesosteosarcomainvasionandmetastasisbyinducingepithelialtomesenchymaltransitionandactivatingthewntbcateninsignalingpathway AT shashahan fibulin3promotesosteosarcomainvasionandmetastasisbyinducingepithelialtomesenchymaltransitionandactivatingthewntbcateninsignalingpathway AT penggao fibulin3promotesosteosarcomainvasionandmetastasisbyinducingepithelialtomesenchymaltransitionandactivatingthewntbcateninsignalingpathway AT changyingliu fibulin3promotesosteosarcomainvasionandmetastasisbyinducingepithelialtomesenchymaltransitionandactivatingthewntbcateninsignalingpathway AT jianminli fibulin3promotesosteosarcomainvasionandmetastasisbyinducingepithelialtomesenchymaltransitionandactivatingthewntbcateninsignalingpathway AT xinpan fibulin3promotesosteosarcomainvasionandmetastasisbyinducingepithelialtomesenchymaltransitionandactivatingthewntbcateninsignalingpathway
_version_	1718385090810085376

Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway

Ejemplares similares