Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel

Here the authors use nanovesicles, microfluidics and single-molecule methods to observe individual ligand binding events that stimulate activation of CNG ion channels. They find that binding is followed by a conformational change of either independent or weakly cooperative binding domains.

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Autores principales:	Vishal R. Patel, Arturo M. Salinas, Darong Qi, Shipra Gupta, David J. Sidote, Marcel P. Goldschen-Ohm
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5a63599f8330436c8f5e11319dd83461
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spelling	oai:doaj.org-article:5a63599f8330436c8f5e11319dd834612021-11-14T12:36:05ZSingle-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel10.1038/s41467-021-26816-52041-1723https://doaj.org/article/5a63599f8330436c8f5e11319dd834612021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26816-5https://doaj.org/toc/2041-1723Here the authors use nanovesicles, microfluidics and single-molecule methods to observe individual ligand binding events that stimulate activation of CNG ion channels. They find that binding is followed by a conformational change of either independent or weakly cooperative binding domains.Vishal R. PatelArturo M. SalinasDarong QiShipra GuptaDavid J. SidoteMarcel P. Goldschen-OhmNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Vishal R. Patel Arturo M. Salinas Darong Qi Shipra Gupta David J. Sidote Marcel P. Goldschen-Ohm Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel
description	Here the authors use nanovesicles, microfluidics and single-molecule methods to observe individual ligand binding events that stimulate activation of CNG ion channels. They find that binding is followed by a conformational change of either independent or weakly cooperative binding domains.
format	article
author	Vishal R. Patel Arturo M. Salinas Darong Qi Shipra Gupta David J. Sidote Marcel P. Goldschen-Ohm
author_facet	Vishal R. Patel Arturo M. Salinas Darong Qi Shipra Gupta David J. Sidote Marcel P. Goldschen-Ohm
author_sort	Vishal R. Patel
title	Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel
title_short	Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel
title_full	Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel
title_fullStr	Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel
title_full_unstemmed	Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel
title_sort	single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5a63599f8330436c8f5e11319dd83461
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Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel

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