microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.

<h4>Background</h4>The extraordinary invasive capability is a major cause of treatment failure and tumor recurrence in glioma, however, the molecular and cellular mechanisms governing glioma invasion remain poorly understood. Evidence in other cell systems has implicated the regulatory r...

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Autores principales:	Liwen An, Yongjun Liu, Anhua Wu, Yifu Guan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a683826cc374c37a82e3c1442435c69
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spelling	oai:doaj.org-article:5a683826cc374c37a82e3c1442435c692021-11-18T09:03:16ZmicroRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.1932-620310.1371/journal.pone.0069478https://doaj.org/article/5a683826cc374c37a82e3c1442435c692013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936026/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The extraordinary invasive capability is a major cause of treatment failure and tumor recurrence in glioma, however, the molecular and cellular mechanisms governing glioma invasion remain poorly understood. Evidence in other cell systems has implicated the regulatory role of microRNA in cell motility and invasion, which promotes us to investigate the biological functions of miR-124 in glioma in this regard.<h4>Results</h4>We have found that miR-124 is dramatically downregulated in clinical specimen of glioma and is negatively correlated with the tumor pathological grading in the current study. The cells transfected by miR-124 expression vector have demonstrated retarded cell mobility. Using a bioinformatics analysis approach, rho-associated coiled-coil containing protein kinase 1 (ROCK1), a well-known cell mobility-related gene, has been identified as the target of miR-124. A dual-luciferase reporter assay was used to confirm that miR-124 targeted directly the 3'UTR of ROCK1 gene and repressed the ROCK1 expression in U87MG human glioma cell line. Furthermore, experiments have shown that the decreased cell mobility was due to the actin cytoskeleton rearrangements and the reduced cell surface ruffle in U87MG glioma cells. These results are similar to the cellular responses of U87MG glioma cells to the treatment of Y-27632, an inhibitor of ROCK protein. Moreover, a constitutively active ROCK1 in miR-124 over-expressed glioma cells reversed the effects of miR-124. Our results revealed a novel mechanism that miR-124 inhibits glioma cells migration and invasion via ROCK1 downregulation.<h4>Conclusions</h4>These results suggest that miR-124 may function as anti-migration and anti-invasion influence in glioma and provides a potential approach for developing miR-124-based therapeutic strategies for malignant glioma therapy.Liwen AnYongjun LiuAnhua WuYifu GuanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69478 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liwen An Yongjun Liu Anhua Wu Yifu Guan microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.
description	<h4>Background</h4>The extraordinary invasive capability is a major cause of treatment failure and tumor recurrence in glioma, however, the molecular and cellular mechanisms governing glioma invasion remain poorly understood. Evidence in other cell systems has implicated the regulatory role of microRNA in cell motility and invasion, which promotes us to investigate the biological functions of miR-124 in glioma in this regard.<h4>Results</h4>We have found that miR-124 is dramatically downregulated in clinical specimen of glioma and is negatively correlated with the tumor pathological grading in the current study. The cells transfected by miR-124 expression vector have demonstrated retarded cell mobility. Using a bioinformatics analysis approach, rho-associated coiled-coil containing protein kinase 1 (ROCK1), a well-known cell mobility-related gene, has been identified as the target of miR-124. A dual-luciferase reporter assay was used to confirm that miR-124 targeted directly the 3'UTR of ROCK1 gene and repressed the ROCK1 expression in U87MG human glioma cell line. Furthermore, experiments have shown that the decreased cell mobility was due to the actin cytoskeleton rearrangements and the reduced cell surface ruffle in U87MG glioma cells. These results are similar to the cellular responses of U87MG glioma cells to the treatment of Y-27632, an inhibitor of ROCK protein. Moreover, a constitutively active ROCK1 in miR-124 over-expressed glioma cells reversed the effects of miR-124. Our results revealed a novel mechanism that miR-124 inhibits glioma cells migration and invasion via ROCK1 downregulation.<h4>Conclusions</h4>These results suggest that miR-124 may function as anti-migration and anti-invasion influence in glioma and provides a potential approach for developing miR-124-based therapeutic strategies for malignant glioma therapy.
format	article
author	Liwen An Yongjun Liu Anhua Wu Yifu Guan
author_facet	Liwen An Yongjun Liu Anhua Wu Yifu Guan
author_sort	Liwen An
title	microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.
title_short	microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.
title_full	microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.
title_fullStr	microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.
title_full_unstemmed	microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.
title_sort	microrna-124 inhibits migration and invasion by down-regulating rock1 in glioma.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5a683826cc374c37a82e3c1442435c69
work_keys_str_mv	AT liwenan microrna124inhibitsmigrationandinvasionbydownregulatingrock1inglioma AT yongjunliu microrna124inhibitsmigrationandinvasionbydownregulatingrock1inglioma AT anhuawu microrna124inhibitsmigrationandinvasionbydownregulatingrock1inglioma AT yifuguan microrna124inhibitsmigrationandinvasionbydownregulatingrock1inglioma
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microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma.

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