Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia

Abstract. Sanjaya WTA, Khoirunnisa NS, Ismiani A, Hazra F, Santosa DA. 2021. Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia. Biodiversitas 22: 2656-2666. Exploring novel wild-type microbes is very important to give more fl...

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Autores principales:	Wilhelmus Terang Arga Sanjaya, Nur Syafira Khoirunhisa, Sri Ismiani, Fahrizal Hazra, Dwi Andreas Santosa
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	16srrna, asgm, bioremediation, mercury, riverbank Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5a6eb11371c34f0fab47969004ef45a7
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spelling	oai:doaj.org-article:5a6eb11371c34f0fab47969004ef45a72021-11-22T12:16:56ZIsolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia1412-033X2085-472210.13057/biodiv/d220714https://doaj.org/article/5a6eb11371c34f0fab47969004ef45a72021-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8158https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sanjaya WTA, Khoirunnisa NS, Ismiani A, Hazra F, Santosa DA. 2021. Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia. Biodiversitas 22: 2656-2666. Exploring novel wild-type microbes is very important to give more flexibility for bioremediation implementation. It is related to discovering strain with higher detoxification ability and more reliable degradation mechanisms. Moreover, novel strain can be used as genetic material for strain development by molecular genetic engineering and production design formulation. The aims of this experiment were to characterize and identify new mercury-resistant microbes, investigate their capacity to accumulate mercury, and analyze the reducing mercury toxicity in bioassay. Four strains of bacteria selected through the screening stage were characterized for their morphological, biochemical, physiological, and molecular genetic characteristics. Considering their characteristics and mercury resistance levels, there are two selected microbial strains: fungus strain Cladosporium halotolerans Hg32 and the bacterial strain Mycolicibacterium peregrinum Hg37 with a mercury resistance level up to 3000 mg L-1. The C. halotolerans Hg32 could remove mercury with the highest potency up to 90.72% at a mercury concentration of 100 mg L-1, while M. peregrinum Hg37 removes up to 77.10% at mercury concentrations of 10 mg L-1. Toxicity bioassay tests using fish confirmed that C. halotolerans Hg32 and M. peregrinum Hg37 had the ability to detoxify mercury in contaminated water. Both have successfully proven to reduce the mortality rate to below 5%.Wilhelmus Terang Arga SanjayaNur Syafira KhoirunhisaSri IsmianiFahrizal HazraDwi Andreas SantosaMBI & UNS Soloarticle16srrna, asgm, bioremediation, mercury, riverbankBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	16srrna, asgm, bioremediation, mercury, riverbank Biology (General) QH301-705.5
spellingShingle	16srrna, asgm, bioremediation, mercury, riverbank Biology (General) QH301-705.5 Wilhelmus Terang Arga Sanjaya Nur Syafira Khoirunhisa Sri Ismiani Fahrizal Hazra Dwi Andreas Santosa Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia
description	Abstract. Sanjaya WTA, Khoirunnisa NS, Ismiani A, Hazra F, Santosa DA. 2021. Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia. Biodiversitas 22: 2656-2666. Exploring novel wild-type microbes is very important to give more flexibility for bioremediation implementation. It is related to discovering strain with higher detoxification ability and more reliable degradation mechanisms. Moreover, novel strain can be used as genetic material for strain development by molecular genetic engineering and production design formulation. The aims of this experiment were to characterize and identify new mercury-resistant microbes, investigate their capacity to accumulate mercury, and analyze the reducing mercury toxicity in bioassay. Four strains of bacteria selected through the screening stage were characterized for their morphological, biochemical, physiological, and molecular genetic characteristics. Considering their characteristics and mercury resistance levels, there are two selected microbial strains: fungus strain Cladosporium halotolerans Hg32 and the bacterial strain Mycolicibacterium peregrinum Hg37 with a mercury resistance level up to 3000 mg L-1. The C. halotolerans Hg32 could remove mercury with the highest potency up to 90.72% at a mercury concentration of 100 mg L-1, while M. peregrinum Hg37 removes up to 77.10% at mercury concentrations of 10 mg L-1. Toxicity bioassay tests using fish confirmed that C. halotolerans Hg32 and M. peregrinum Hg37 had the ability to detoxify mercury in contaminated water. Both have successfully proven to reduce the mortality rate to below 5%.
format	article
author	Wilhelmus Terang Arga Sanjaya Nur Syafira Khoirunhisa Sri Ismiani Fahrizal Hazra Dwi Andreas Santosa
author_facet	Wilhelmus Terang Arga Sanjaya Nur Syafira Khoirunhisa Sri Ismiani Fahrizal Hazra Dwi Andreas Santosa
author_sort	Wilhelmus Terang Arga Sanjaya
title	Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia
title_short	Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia
title_full	Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia
title_fullStr	Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia
title_full_unstemmed	Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia
title_sort	isolation and characterization of mercury-resistant microbes from gold mine area in mount pongkor, bogor district, indonesia
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/5a6eb11371c34f0fab47969004ef45a7
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Isolation and characterization of mercury-resistant microbes from gold mine area in Mount Pongkor, Bogor District, Indonesia

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