HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats

Anemia is a clinical disorder orchestrated by factors such as drugs, including phenylhydrazine (PHZ). This study explored the protective roles of Copaifera salikounda methanol leaf extract (CSMLE) against PHZ-induced hematotoxicity and oxidative stress in rats. In vitro antioxidants studies of CSMLE...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chinyere Aloke, Chinedum Uche Emelike, Nwogo Ajuka Obasi, Patience Nkemjika Ogbu, Chuks Oswald Edeogu, Chinedu Godwin Uzomba, Ofovwe Ekakitie, Adeolu Adewale Iyaniwura, Chukwubuike Cyprian Okoro, Blessing Peter Okey, Glory Ginikachukwu Aninjoku, Bature Charles Ushahemba
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Phenylhydrazine Oxidative stress Hematotoxicity Copaifera salikounda Spleenotoxic effects Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/5a6fd176f01f44a483b241cd6b48ee7a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5a6fd176f01f44a483b241cd6b48ee7a
record_format	dspace
spelling	oai:doaj.org-article:5a6fd176f01f44a483b241cd6b48ee7a2021-11-20T04:57:51ZHPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats1878-535210.1016/j.arabjc.2021.103428https://doaj.org/article/5a6fd176f01f44a483b241cd6b48ee7a2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1878535221004433https://doaj.org/toc/1878-5352Anemia is a clinical disorder orchestrated by factors such as drugs, including phenylhydrazine (PHZ). This study explored the protective roles of Copaifera salikounda methanol leaf extract (CSMLE) against PHZ-induced hematotoxicity and oxidative stress in rats. In vitro antioxidants studies of CSMLE using 1,1-diphenyl-2-picryl hydrazyl (DPPH) and ferric reducing antioxidant potential (FRAP) and quantification of CSMLE bioactive compounds using High-Performance-Liquid-Chromatography (HPLC) were done. In vivo, thirty rats in five groups (n = 6) were used. Anemia was induced intraperitoneally in groups 2 to 5 with 40 mg/kg body-weight/day PHZ for two days. Group 1 received normal saline, group 2 (untreated: negative-control), group 3 received 1 ml/kg-body-weight Vit B12 syrup while groups 4 and 5 received 200 mg/kg and 400 mg/kg-body-weight CSMLE, respectively, daily for fourteen days. Result of CSMLE profiling showed luteolin (76.49 ng/Ml) and squalene (38.31 ng/Ml) as the most abundant flavonoid and terpenoid, respectively. In vitro CSMLE demonstrated dose-dependent antioxidant effects on DPPH and FRAP with IC50 of 0.1085 mg/ml and 0.07344 mg/ml (EC50), respectively. In vivo, CSMLE improved the body weight, red blood cell, hematocrit and hemoglobin concentrations, significantly (P < 0.05), reduced serum malondialdehyde and nitic oxide but increased reduced glutathione levels, superoxide dismutase and catalase activities and attenuated the levels of Interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in dose-dependent manner in treated groups relative to the negative control. The spleen tissues histology results corroborated biochemical results. The results indicated that CSMLE possesses anti-anemic potentials and attenuate spleenotoxic effects of PHZ.Chinyere AlokeChinedum Uche EmelikeNwogo Ajuka ObasiPatience Nkemjika OgbuChuks Oswald EdeoguChinedu Godwin UzombaOfovwe EkakitieAdeolu Adewale IyaniwuraChukwubuike Cyprian OkoroBlessing Peter OkeyGlory Ginikachukwu AninjokuBature Charles UshahembaElsevierarticlePhenylhydrazineOxidative stressHematotoxicityCopaifera salikoundaSpleenotoxic effectsChemistryQD1-999ENArabian Journal of Chemistry, Vol 14, Iss 12, Pp 103428- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Phenylhydrazine Oxidative stress Hematotoxicity Copaifera salikounda Spleenotoxic effects Chemistry QD1-999
spellingShingle	Phenylhydrazine Oxidative stress Hematotoxicity Copaifera salikounda Spleenotoxic effects Chemistry QD1-999 Chinyere Aloke Chinedum Uche Emelike Nwogo Ajuka Obasi Patience Nkemjika Ogbu Chuks Oswald Edeogu Chinedu Godwin Uzomba Ofovwe Ekakitie Adeolu Adewale Iyaniwura Chukwubuike Cyprian Okoro Blessing Peter Okey Glory Ginikachukwu Aninjoku Bature Charles Ushahemba HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats
description	Anemia is a clinical disorder orchestrated by factors such as drugs, including phenylhydrazine (PHZ). This study explored the protective roles of Copaifera salikounda methanol leaf extract (CSMLE) against PHZ-induced hematotoxicity and oxidative stress in rats. In vitro antioxidants studies of CSMLE using 1,1-diphenyl-2-picryl hydrazyl (DPPH) and ferric reducing antioxidant potential (FRAP) and quantification of CSMLE bioactive compounds using High-Performance-Liquid-Chromatography (HPLC) were done. In vivo, thirty rats in five groups (n = 6) were used. Anemia was induced intraperitoneally in groups 2 to 5 with 40 mg/kg body-weight/day PHZ for two days. Group 1 received normal saline, group 2 (untreated: negative-control), group 3 received 1 ml/kg-body-weight Vit B12 syrup while groups 4 and 5 received 200 mg/kg and 400 mg/kg-body-weight CSMLE, respectively, daily for fourteen days. Result of CSMLE profiling showed luteolin (76.49 ng/Ml) and squalene (38.31 ng/Ml) as the most abundant flavonoid and terpenoid, respectively. In vitro CSMLE demonstrated dose-dependent antioxidant effects on DPPH and FRAP with IC50 of 0.1085 mg/ml and 0.07344 mg/ml (EC50), respectively. In vivo, CSMLE improved the body weight, red blood cell, hematocrit and hemoglobin concentrations, significantly (P < 0.05), reduced serum malondialdehyde and nitic oxide but increased reduced glutathione levels, superoxide dismutase and catalase activities and attenuated the levels of Interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in dose-dependent manner in treated groups relative to the negative control. The spleen tissues histology results corroborated biochemical results. The results indicated that CSMLE possesses anti-anemic potentials and attenuate spleenotoxic effects of PHZ.
format	article
author	Chinyere Aloke Chinedum Uche Emelike Nwogo Ajuka Obasi Patience Nkemjika Ogbu Chuks Oswald Edeogu Chinedu Godwin Uzomba Ofovwe Ekakitie Adeolu Adewale Iyaniwura Chukwubuike Cyprian Okoro Blessing Peter Okey Glory Ginikachukwu Aninjoku Bature Charles Ushahemba
author_facet	Chinyere Aloke Chinedum Uche Emelike Nwogo Ajuka Obasi Patience Nkemjika Ogbu Chuks Oswald Edeogu Chinedu Godwin Uzomba Ofovwe Ekakitie Adeolu Adewale Iyaniwura Chukwubuike Cyprian Okoro Blessing Peter Okey Glory Ginikachukwu Aninjoku Bature Charles Ushahemba
author_sort	Chinyere Aloke
title	HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats
title_short	HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats
title_full	HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats
title_fullStr	HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats
title_full_unstemmed	HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats
title_sort	hplc profiling and studies on copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5a6fd176f01f44a483b241cd6b48ee7a
work_keys_str_mv	AT chinyerealoke hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT chinedumucheemelike hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT nwogoajukaobasi hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT patiencenkemjikaogbu hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT chuksoswaldedeogu hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT chinedugodwinuzomba hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT ofovweekakitie hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT adeoluadewaleiyaniwura hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT chukwubuikecyprianokoro hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT blessingpeterokey hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT gloryginikachukwuaninjoku hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats AT baturecharlesushahemba hplcprofilingandstudiesoncopaiferasalikoundamethanolleafextractonphenylhydrazineinducedhematotoxicityandoxidativestressinrats
_version_	1718419716060479488

HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats

Ejemplares similares