Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci

Abstract Streptococcus gallolyticus LL009 produces gallocin D, a narrow spectrum two component bacteriocin with potent activity against vancomycin-resistant enterococci. Gallocin D is distinct from gallocin A, a separate two component bacteriocin produced by S. gallolyticus. Although the gene cluste...

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Autores principales:	Daragh Hill, Paula M. O’Connor, Eric Altermann, Li Day, Colin Hill, Catherine Stanton, R. Paul Ross
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a7ab2021620431b8b09b3f9a2ed97e4
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spelling	oai:doaj.org-article:5a7ab2021620431b8b09b3f9a2ed97e42021-12-02T16:43:38ZExtensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci10.1038/s41598-020-70328-z2045-2322https://doaj.org/article/5a7ab2021620431b8b09b3f9a2ed97e42020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-70328-zhttps://doaj.org/toc/2045-2322Abstract Streptococcus gallolyticus LL009 produces gallocin D, a narrow spectrum two component bacteriocin with potent activity against vancomycin-resistant enterococci. Gallocin D is distinct from gallocin A, a separate two component bacteriocin produced by S. gallolyticus. Although the gene clusters encoding gallocin A and gallocin D have a high degree of gene synteny, the structural genes are highly variable and appear to have undergone gene shuffling with other streptococcal species. Gallocin D was analysed in laboratory-based experiments. The mature peptides are 3,343 ± 1 Da and 3,019 ± 1 Da and could be readily synthesized and display activity against a vancomycin resistant Enterococcus strain EC300 with a MIC value of 1.56 µM. Importantly, these bacteriocins could contribute to the ability of S. gallolyticus to colonize the colon where they have been associated with colorectal cancer.Daragh HillPaula M. O’ConnorEric AltermannLi DayColin HillCatherine StantonR. Paul RossNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daragh Hill Paula M. O’Connor Eric Altermann Li Day Colin Hill Catherine Stanton R. Paul Ross Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci
description	Abstract Streptococcus gallolyticus LL009 produces gallocin D, a narrow spectrum two component bacteriocin with potent activity against vancomycin-resistant enterococci. Gallocin D is distinct from gallocin A, a separate two component bacteriocin produced by S. gallolyticus. Although the gene clusters encoding gallocin A and gallocin D have a high degree of gene synteny, the structural genes are highly variable and appear to have undergone gene shuffling with other streptococcal species. Gallocin D was analysed in laboratory-based experiments. The mature peptides are 3,343 ± 1 Da and 3,019 ± 1 Da and could be readily synthesized and display activity against a vancomycin resistant Enterococcus strain EC300 with a MIC value of 1.56 µM. Importantly, these bacteriocins could contribute to the ability of S. gallolyticus to colonize the colon where they have been associated with colorectal cancer.
format	article
author	Daragh Hill Paula M. O’Connor Eric Altermann Li Day Colin Hill Catherine Stanton R. Paul Ross
author_facet	Daragh Hill Paula M. O’Connor Eric Altermann Li Day Colin Hill Catherine Stanton R. Paul Ross
author_sort	Daragh Hill
title	Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci
title_short	Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci
title_full	Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci
title_fullStr	Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci
title_full_unstemmed	Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci
title_sort	extensive bacteriocin gene shuffling in the streptococcus bovis/streptococcus equinus complex reveals gallocin d with activity against vancomycin resistant enterococci
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5a7ab2021620431b8b09b3f9a2ed97e4
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Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci

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