Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria

RNA synthesis by bacterial RNA polymerase is interrupted by pauses but their role in RNA synthesis is poorly understood. Here the authors use single-molecule FRET and biochemical analysis to show that pausing regulates branching between the abortive and productive outcomes of initial transcription.

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Autores principales:	David Dulin, David L. V. Bauer, Anssi M. Malinen, Jacob J. W. Bakermans, Martin Kaller, Zakia Morichaud, Ivan Petushkov, Martin Depken, Konstantin Brodolin, Andrey Kulbachinskiy, Achillefs N. Kapanidis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5a7bdeadb61d4f6d961109f5e5564849
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spelling	oai:doaj.org-article:5a7bdeadb61d4f6d961109f5e55648492021-12-02T14:38:51ZPausing controls branching between productive and non-productive pathways during initial transcription in bacteria10.1038/s41467-018-03902-92041-1723https://doaj.org/article/5a7bdeadb61d4f6d961109f5e55648492018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03902-9https://doaj.org/toc/2041-1723RNA synthesis by bacterial RNA polymerase is interrupted by pauses but their role in RNA synthesis is poorly understood. Here the authors use single-molecule FRET and biochemical analysis to show that pausing regulates branching between the abortive and productive outcomes of initial transcription.David DulinDavid L. V. BauerAnssi M. MalinenJacob J. W. BakermansMartin KallerZakia MorichaudIvan PetushkovMartin DepkenKonstantin BrodolinAndrey KulbachinskiyAchillefs N. KapanidisNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q David Dulin David L. V. Bauer Anssi M. Malinen Jacob J. W. Bakermans Martin Kaller Zakia Morichaud Ivan Petushkov Martin Depken Konstantin Brodolin Andrey Kulbachinskiy Achillefs N. Kapanidis Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
description	RNA synthesis by bacterial RNA polymerase is interrupted by pauses but their role in RNA synthesis is poorly understood. Here the authors use single-molecule FRET and biochemical analysis to show that pausing regulates branching between the abortive and productive outcomes of initial transcription.
format	article
author	David Dulin David L. V. Bauer Anssi M. Malinen Jacob J. W. Bakermans Martin Kaller Zakia Morichaud Ivan Petushkov Martin Depken Konstantin Brodolin Andrey Kulbachinskiy Achillefs N. Kapanidis
author_facet	David Dulin David L. V. Bauer Anssi M. Malinen Jacob J. W. Bakermans Martin Kaller Zakia Morichaud Ivan Petushkov Martin Depken Konstantin Brodolin Andrey Kulbachinskiy Achillefs N. Kapanidis
author_sort	David Dulin
title	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_short	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_full	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_fullStr	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_full_unstemmed	Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
title_sort	pausing controls branching between productive and non-productive pathways during initial transcription in bacteria
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5a7bdeadb61d4f6d961109f5e5564849
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Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria

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