Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light

Glioblastoma is one of the most aggressive types of tumors. Although few treatment options are currently available, new modalities are needed to improve prognosis. In this context, photodynamic therapy (PDT) is a promising adjuvant treatment modality. In the present work, hypericin-mediated PDT (hyp...

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Autores principales:	Viktoria Pevna, Georges Wagnières, Veronika Huntosova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	glioblastoma cells autophagy photodynamic therapy photobiomodulation apoptosis microscopy Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5a7d69b034f141ea91dff5449db815a4
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spelling	oai:doaj.org-article:5a7d69b034f141ea91dff5449db815a42021-11-25T16:51:00ZAutophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light10.3390/biomedicines91117032227-9059https://doaj.org/article/5a7d69b034f141ea91dff5449db815a42021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1703https://doaj.org/toc/2227-9059Glioblastoma is one of the most aggressive types of tumors. Although few treatment options are currently available, new modalities are needed to improve prognosis. In this context, photodynamic therapy (PDT) is a promising adjuvant treatment modality. In the present work, hypericin-mediated PDT (hypericin-PDT, 2 J/cm<sup>2</sup>) of U87 MG cells is combined with (2 min, 15 mW/cm<sup>2</sup> at 808 nm) photobiomodulation (PBM). We observed that PBM stimulates autophagy, which, in combination with PDT, increases the treatment efficacy and leads to apoptosis. Confocal fluorescence microscopy, cytotoxicity assays and Western blot were used to monitor apoptotic and autophagic processes in these cells. Destabilization of lysosomes, mitochondria and the Golgi apparatus led to an increase in lactate dehydrogenase activity, oxidative stress levels, LC3-II, and caspase-3, as well as a decrease of the PKCα and STAT3 protein levels in response to hypericin-PDT subcellular concentration in U87 MG cells. Our results indicate that therapeutic hypericin concentrations can be reduced when PDT is combined with PBM. This will likely allow to reduce the damage induced in surrounding healthy tissues when PBM-hypericin-PDT is used for in vivo tumor treatments.Viktoria PevnaGeorges WagnièresVeronika HuntosovaMDPI AGarticleglioblastoma cellsautophagyphotodynamic therapyphotobiomodulationapoptosismicroscopyBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1703, p 1703 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioblastoma cells autophagy photodynamic therapy photobiomodulation apoptosis microscopy Biology (General) QH301-705.5
spellingShingle	glioblastoma cells autophagy photodynamic therapy photobiomodulation apoptosis microscopy Biology (General) QH301-705.5 Viktoria Pevna Georges Wagnières Veronika Huntosova Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light
description	Glioblastoma is one of the most aggressive types of tumors. Although few treatment options are currently available, new modalities are needed to improve prognosis. In this context, photodynamic therapy (PDT) is a promising adjuvant treatment modality. In the present work, hypericin-mediated PDT (hypericin-PDT, 2 J/cm<sup>2</sup>) of U87 MG cells is combined with (2 min, 15 mW/cm<sup>2</sup> at 808 nm) photobiomodulation (PBM). We observed that PBM stimulates autophagy, which, in combination with PDT, increases the treatment efficacy and leads to apoptosis. Confocal fluorescence microscopy, cytotoxicity assays and Western blot were used to monitor apoptotic and autophagic processes in these cells. Destabilization of lysosomes, mitochondria and the Golgi apparatus led to an increase in lactate dehydrogenase activity, oxidative stress levels, LC3-II, and caspase-3, as well as a decrease of the PKCα and STAT3 protein levels in response to hypericin-PDT subcellular concentration in U87 MG cells. Our results indicate that therapeutic hypericin concentrations can be reduced when PDT is combined with PBM. This will likely allow to reduce the damage induced in surrounding healthy tissues when PBM-hypericin-PDT is used for in vivo tumor treatments.
format	article
author	Viktoria Pevna Georges Wagnières Veronika Huntosova
author_facet	Viktoria Pevna Georges Wagnières Veronika Huntosova
author_sort	Viktoria Pevna
title	Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light
title_short	Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light
title_full	Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light
title_fullStr	Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light
title_full_unstemmed	Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light
title_sort	autophagy and apoptosis induced in u87 mg glioblastoma cells by hypericin-mediated photodynamic therapy can be photobiomodulated with 808 nm light
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5a7d69b034f141ea91dff5449db815a4
work_keys_str_mv	AT viktoriapevna autophagyandapoptosisinducedinu87mgglioblastomacellsbyhypericinmediatedphotodynamictherapycanbephotobiomodulatedwith808nmlight AT georgeswagnieres autophagyandapoptosisinducedinu87mgglioblastomacellsbyhypericinmediatedphotodynamictherapycanbephotobiomodulatedwith808nmlight AT veronikahuntosova autophagyandapoptosisinducedinu87mgglioblastomacellsbyhypericinmediatedphotodynamictherapycanbephotobiomodulatedwith808nmlight
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Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light

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