Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress

Abstract Lactobacillus acetotolerans is a major microbe contributing to the Chinese liquor fermentation with unknown function. It can be grown well in a high concentration of ethanol. RNA sequencing (RNA-seq) was performed on L. acetotolerans F28 growing in 12% ethanol to determine important genetic...

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Autores principales:	Xiaopan Yang, Kunling Teng, Jie Zhang, Fangfang Wang, Tong Zhang, Guomin Ai, Peijie Han, Fengyan Bai, Jin Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a852f77abfc43fa85bf1d8ca9819dac
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spelling	oai:doaj.org-article:5a852f77abfc43fa85bf1d8ca9819dac2021-12-02T12:32:02ZTranscriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress10.1038/s41598-017-02975-82045-2322https://doaj.org/article/5a852f77abfc43fa85bf1d8ca9819dac2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02975-8https://doaj.org/toc/2045-2322Abstract Lactobacillus acetotolerans is a major microbe contributing to the Chinese liquor fermentation with unknown function. It can be grown well in a high concentration of ethanol. RNA sequencing (RNA-seq) was performed on L. acetotolerans F28 growing in 12% ethanol to determine important genetic mechanisms for both a short and long term adaption to this environment. A genome-wide transcriptional analysis revealed that the most important genetic elements for L. acetotolerans F28 grown in ethanol are related to high levels of stress response and fatty acid biosynthesis, and a reduction of amino acid transport and metabolism after both a short and long time stress. The fatty acid methyl ester analyses showed that most fatty acids were increased in L. acetotolerans F28 after exposure to ethanol while the unsaturated fatty acid octadecenoic acid (C18:1) was significantly increased. The increasing unsaturated fatty acid biosynthesis in L. acetotolerans F28 might enhance cell membrane fluidity and protect the cells against high concentration of ethanol. Overall, the transcriptome and functional analysis indicated that the elevated stress response and fatty acid biosynthesis, and the decrease of amino acid transport and metabolism might play important roles for L. acetotolerans F28 to adapt to environmental ethanol.Xiaopan YangKunling TengJie ZhangFangfang WangTong ZhangGuomin AiPeijie HanFengyan BaiJin ZhongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaopan Yang Kunling Teng Jie Zhang Fangfang Wang Tong Zhang Guomin Ai Peijie Han Fengyan Bai Jin Zhong Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress
description	Abstract Lactobacillus acetotolerans is a major microbe contributing to the Chinese liquor fermentation with unknown function. It can be grown well in a high concentration of ethanol. RNA sequencing (RNA-seq) was performed on L. acetotolerans F28 growing in 12% ethanol to determine important genetic mechanisms for both a short and long term adaption to this environment. A genome-wide transcriptional analysis revealed that the most important genetic elements for L. acetotolerans F28 grown in ethanol are related to high levels of stress response and fatty acid biosynthesis, and a reduction of amino acid transport and metabolism after both a short and long time stress. The fatty acid methyl ester analyses showed that most fatty acids were increased in L. acetotolerans F28 after exposure to ethanol while the unsaturated fatty acid octadecenoic acid (C18:1) was significantly increased. The increasing unsaturated fatty acid biosynthesis in L. acetotolerans F28 might enhance cell membrane fluidity and protect the cells against high concentration of ethanol. Overall, the transcriptome and functional analysis indicated that the elevated stress response and fatty acid biosynthesis, and the decrease of amino acid transport and metabolism might play important roles for L. acetotolerans F28 to adapt to environmental ethanol.
format	article
author	Xiaopan Yang Kunling Teng Jie Zhang Fangfang Wang Tong Zhang Guomin Ai Peijie Han Fengyan Bai Jin Zhong
author_facet	Xiaopan Yang Kunling Teng Jie Zhang Fangfang Wang Tong Zhang Guomin Ai Peijie Han Fengyan Bai Jin Zhong
author_sort	Xiaopan Yang
title	Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress
title_short	Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress
title_full	Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress
title_fullStr	Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress
title_full_unstemmed	Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress
title_sort	transcriptome responses of lactobacillus acetotolerans f28 to a short and long term ethanol stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5a852f77abfc43fa85bf1d8ca9819dac
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Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress

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