Determination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats

BACKGROUND AND OBJECTIVE: Organophosphorus (OP) compounds are highly toxic and are widely used as an insecticide in agriculture and domestic consumptions. Acetylcholinesterase (AChE) inhibition is the primary mechanism of acute in vivo toxicity of organophosphorus compounds. In the present study we...

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Autores principales:	M Mohammadi, E Ghani, E Ghasemi, A Khoshbaten, AR Asgari
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2010
Materias:	paraoxon acetylcholinesterase recovery cerebral cortex hippocampus Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5a88629ac5ec48d8a1c9d2ca3c41b23d
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spelling	oai:doaj.org-article:5a88629ac5ec48d8a1c9d2ca3c41b23d2021-11-10T09:00:14ZDetermination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats1561-41072251-7170https://doaj.org/article/5a88629ac5ec48d8a1c9d2ca3c41b23d2010-04-01T00:00:00Zhttp://jbums.org/article-1-3406-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Organophosphorus (OP) compounds are highly toxic and are widely used as an insecticide in agriculture and domestic consumptions. Acetylcholinesterase (AChE) inhibition is the primary mechanism of acute in vivo toxicity of organophosphorus compounds. In the present study we evaluated inhibition and recovery of the plasma, cerebral cortex and hippocampus AChE activity and their correlations following systemic administration of three doses of paraoxon in three different time points in rat. METHODS: Eighty four male Wistar rats (200-270 g) were used in this study and divided into groups of seven. Animals were given a single intraperitoneal injection of corn oil (vehicle group) or one of the doses of paraoxon (0.1, 0.3, or 0.7 mg/kg) and AChE activity in the plasma, cerebral cortex, and hippocampus was measured at 30 min, 4 h, and 18 h after exposure using the modified method of Ellman.FINDINGS: Plasma and brain AChE activity was inhibited in a dose dependent manner by paraoxon. After 18 h, plasma AChE activity was recovered 32, and 42 percent (p<0.001) in animals exposed to 0.3, and 0.7 mg/kg paraoxon, respectively. 18 h after 0.7 mg/kg paraoxon, AChE activity was significantly recovered (p<0.001) in both brain areas (about 20%). Plasma AChE activity correlated significantly with both cerebral cortex and hippocampus AChE activity in rats treated with paraoxon (0.3 and 0.7 mg/kg).CONCLUSION: In both brain areas, paraoxon (only 0.7 mg/kg) inhibited AChE activity to induce seizure activity after 30 min. Inhibition of the plasma AChE activity can use as a marker of exposure only in severe toxicities with OP compounds.M Mohammadi,E Ghani,E GhasemiA Khoshbaten,AR AsgariBabol University of Medical Sciencesarticleparaoxonacetylcholinesteraserecoverycerebral cortexhippocampusMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 12, Iss 1, Pp 8-15 (2010)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	paraoxon acetylcholinesterase recovery cerebral cortex hippocampus Medicine R Medicine (General) R5-920
spellingShingle	paraoxon acetylcholinesterase recovery cerebral cortex hippocampus Medicine R Medicine (General) R5-920 M Mohammadi, E Ghani, E Ghasemi A Khoshbaten, AR Asgari Determination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats
description	BACKGROUND AND OBJECTIVE: Organophosphorus (OP) compounds are highly toxic and are widely used as an insecticide in agriculture and domestic consumptions. Acetylcholinesterase (AChE) inhibition is the primary mechanism of acute in vivo toxicity of organophosphorus compounds. In the present study we evaluated inhibition and recovery of the plasma, cerebral cortex and hippocampus AChE activity and their correlations following systemic administration of three doses of paraoxon in three different time points in rat. METHODS: Eighty four male Wistar rats (200-270 g) were used in this study and divided into groups of seven. Animals were given a single intraperitoneal injection of corn oil (vehicle group) or one of the doses of paraoxon (0.1, 0.3, or 0.7 mg/kg) and AChE activity in the plasma, cerebral cortex, and hippocampus was measured at 30 min, 4 h, and 18 h after exposure using the modified method of Ellman.FINDINGS: Plasma and brain AChE activity was inhibited in a dose dependent manner by paraoxon. After 18 h, plasma AChE activity was recovered 32, and 42 percent (p<0.001) in animals exposed to 0.3, and 0.7 mg/kg paraoxon, respectively. 18 h after 0.7 mg/kg paraoxon, AChE activity was significantly recovered (p<0.001) in both brain areas (about 20%). Plasma AChE activity correlated significantly with both cerebral cortex and hippocampus AChE activity in rats treated with paraoxon (0.3 and 0.7 mg/kg).CONCLUSION: In both brain areas, paraoxon (only 0.7 mg/kg) inhibited AChE activity to induce seizure activity after 30 min. Inhibition of the plasma AChE activity can use as a marker of exposure only in severe toxicities with OP compounds.
format	article
author	M Mohammadi, E Ghani, E Ghasemi A Khoshbaten, AR Asgari
author_facet	M Mohammadi, E Ghani, E Ghasemi A Khoshbaten, AR Asgari
author_sort	M Mohammadi,
title	Determination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats
title_short	Determination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats
title_full	Determination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats
title_fullStr	Determination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats
title_full_unstemmed	Determination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats
title_sort	determination of the inhibition and recovery of the plasma, cerebral cortex and hippocampus acetylcholinesterase activity in male paraoxon-treated rats
publisher	Babol University of Medical Sciences
publishDate	2010
url	https://doaj.org/article/5a88629ac5ec48d8a1c9d2ca3c41b23d
work_keys_str_mv	AT mmohammadi determinationoftheinhibitionandrecoveryoftheplasmacerebralcortexandhippocampusacetylcholinesteraseactivityinmaleparaoxontreatedrats AT eghani determinationoftheinhibitionandrecoveryoftheplasmacerebralcortexandhippocampusacetylcholinesteraseactivityinmaleparaoxontreatedrats AT eghasemi determinationoftheinhibitionandrecoveryoftheplasmacerebralcortexandhippocampusacetylcholinesteraseactivityinmaleparaoxontreatedrats AT akhoshbaten determinationoftheinhibitionandrecoveryoftheplasmacerebralcortexandhippocampusacetylcholinesteraseactivityinmaleparaoxontreatedrats AT arasgari determinationoftheinhibitionandrecoveryoftheplasmacerebralcortexandhippocampusacetylcholinesteraseactivityinmaleparaoxontreatedrats
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Determination of the Inhibition and Recovery of the Plasma, Cerebral Cortex and Hippocampus Acetylcholinesterase Activity in Male Paraoxon-Treated Rats

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