Expression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine

Background. Mechanisms are known that allow partial local regulation of the thyroid status of tissues using peripheral conversion of iodothyronines, which determines the local level of metabolic activity. The molecular mechanisms of degenerative-dystrophic changes in the ligamentum flavum are not we...

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Autores principales:	L. V. Rodionova, L. G. Samoilova, A. V. Nevezhina, I. A. Shurygina
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2019
Materias:	stenosis of the spinal canal peripheral conversion iodothyronenes thyroxine triiodothyronine deiodinase ligamentum flavum Science Q
Acceso en línea:	https://doaj.org/article/5a95be8d2d5742a7a2adbb3f17923bbf
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spelling	oai:doaj.org-article:5a95be8d2d5742a7a2adbb3f17923bbf2021-11-23T06:14:43ZExpression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine2541-94202587-959610.29413/ABS.2019-4.6.3https://doaj.org/article/5a95be8d2d5742a7a2adbb3f17923bbf2019-12-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2190https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Mechanisms are known that allow partial local regulation of the thyroid status of tissues using peripheral conversion of iodothyronines, which determines the local level of metabolic activity. The molecular mechanisms of degenerative-dystrophic changes in the ligamentum flavum are not well understood; only a few studies on the expression of genes in this substrate have been published.The aim of the study was to identify key factors in the peripheral conversion of iodothyronines in ligamentum flavum in patients with stenosis of spinal canal and dural sac in the lumbar spine and to evaluate the features of the expression of deiodinase genes depending on the severity of ossification processes of ligamentum flavum.Methods. A group of patients with stenotic processes of the spinal canal was examined: 31 people (15 men, 16 women). The average age was45.73 ± 1.95years. In intraoperatively collected samples of ligamentum flavum, the expression ofdeiodinase genes (real time PCR) was studied and the severity of ossification processes was evaluated using a histopathological study.Results. The key factors of peripheral conversion of iodothyronines in intraoperative bioptates of the ligamentum flavum of patients (n = 31) with stenosis of the spinal canal at the lumbar level were revealed. Information was obtained on the local level of metabolic activity in the focus of pathology. The intensity and variability of deiodinase gene expression in ligamentum flavum samples were evaluated. The distribution of deiodinase gene expression patterns in groups formed on the basis of presence/absence of ossification of ligamentum flavum was found. Various variants of peripheral conversion of iodothyronines in biopsy samples of the yellow ligament of the operated patients were determined and the frequency of their occurrence was estimated.Conclusion. The key factors ofperipheral conversion of iodothyronines in intraoperative biopsy samples of the ligamentum flavum of patients with degenerative diseases of the spine were revealed for the first time. The reasons for the differences in tissue metabolism of iodothyronines and the place in the pathogenesis of stenotic processes are yet to be found.L. V. RodionovaL. G. SamoilovaA. V. NevezhinaI. A. ShuryginaScientific Сentre for Family Health and Human Reproduction Problemsarticlestenosis of the spinal canalperipheral conversion iodothyronenesthyroxinetriiodothyroninedeiodinaseligamentum flavumScienceQRUActa Biomedica Scientifica, Vol 4, Iss 6, Pp 20-25 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	stenosis of the spinal canal peripheral conversion iodothyronenes thyroxine triiodothyronine deiodinase ligamentum flavum Science Q
spellingShingle	stenosis of the spinal canal peripheral conversion iodothyronenes thyroxine triiodothyronine deiodinase ligamentum flavum Science Q L. V. Rodionova L. G. Samoilova A. V. Nevezhina I. A. Shurygina Expression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine
description	Background. Mechanisms are known that allow partial local regulation of the thyroid status of tissues using peripheral conversion of iodothyronines, which determines the local level of metabolic activity. The molecular mechanisms of degenerative-dystrophic changes in the ligamentum flavum are not well understood; only a few studies on the expression of genes in this substrate have been published.The aim of the study was to identify key factors in the peripheral conversion of iodothyronines in ligamentum flavum in patients with stenosis of spinal canal and dural sac in the lumbar spine and to evaluate the features of the expression of deiodinase genes depending on the severity of ossification processes of ligamentum flavum.Methods. A group of patients with stenotic processes of the spinal canal was examined: 31 people (15 men, 16 women). The average age was45.73 ± 1.95years. In intraoperatively collected samples of ligamentum flavum, the expression ofdeiodinase genes (real time PCR) was studied and the severity of ossification processes was evaluated using a histopathological study.Results. The key factors of peripheral conversion of iodothyronines in intraoperative bioptates of the ligamentum flavum of patients (n = 31) with stenosis of the spinal canal at the lumbar level were revealed. Information was obtained on the local level of metabolic activity in the focus of pathology. The intensity and variability of deiodinase gene expression in ligamentum flavum samples were evaluated. The distribution of deiodinase gene expression patterns in groups formed on the basis of presence/absence of ossification of ligamentum flavum was found. Various variants of peripheral conversion of iodothyronines in biopsy samples of the yellow ligament of the operated patients were determined and the frequency of their occurrence was estimated.Conclusion. The key factors ofperipheral conversion of iodothyronines in intraoperative biopsy samples of the ligamentum flavum of patients with degenerative diseases of the spine were revealed for the first time. The reasons for the differences in tissue metabolism of iodothyronines and the place in the pathogenesis of stenotic processes are yet to be found.
format	article
author	L. V. Rodionova L. G. Samoilova A. V. Nevezhina I. A. Shurygina
author_facet	L. V. Rodionova L. G. Samoilova A. V. Nevezhina I. A. Shurygina
author_sort	L. V. Rodionova
title	Expression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine
title_short	Expression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine
title_full	Expression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine
title_fullStr	Expression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine
title_full_unstemmed	Expression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine
title_sort	expression of deiodinase genes in intraoperative samples of <i>ligamentum flavum</i>ligamentum flavum in patients with stenotic processes of the spinal canal and dural sac on the lumbar spine
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2019
url	https://doaj.org/article/5a95be8d2d5742a7a2adbb3f17923bbf
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Expression of Deiodinase Genes in Intraoperative Samples of <i>Ligamentum Flavum</i>Ligamentum Flavum in Patients with Stenotic Processes of the Spinal Canal and Dural Sac on the Lumbar Spine

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