A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans

Abstract Almost all organisms maintain a circadian clock from birth to death to synchronize their own physiology and behavior with the earth’s rotation. Because the in vivo evaluation of human circadian characteristics is labor-intensive, in vitro or ex vivo approaches could provide advantages. In t...

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Autores principales:	Ai Yamaguchi, Ritsuko Matsumura, Takashi Matsuzaki, Wataru Nakamura, Koichi Node, Makoto Akashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5aa270178b3640fe95051c018f962e05
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spelling	oai:doaj.org-article:5aa270178b3640fe95051c018f962e052021-12-02T15:04:57ZA simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans10.1038/s41598-017-07268-82045-2322https://doaj.org/article/5aa270178b3640fe95051c018f962e052017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07268-8https://doaj.org/toc/2045-2322Abstract Almost all organisms maintain a circadian clock from birth to death to synchronize their own physiology and behavior with the earth’s rotation. Because the in vivo evaluation of human circadian characteristics is labor-intensive, in vitro or ex vivo approaches could provide advantages. In this study, to enable the simple and non-invasive evaluation of autonomous circadian oscillation, we established a method for monitoring clock gene expression by performing ex vivo culture of whole hair root tissue. This method is extremely simple and imposes little burden on subjects. Results obtained using Cryptochrome-deficient mice support that circadian period length in hair tissue correlates with intrinsic period length observed in physiology and behavior. We then applied this method to old-old subjects with severe dementia, who showed abnormal circadian behavior, and found that their peripheral clocks autonomously oscillated in a manner similar to those of healthy or younger subjects, indicating that the effect of cellular senescence on the autonomous clock oscillator is limited at least in some cell types. Although further validation may be required, the hair tissue-based culture assay would be a tool to investigate intrinsic circadian characteristics in humans.Ai YamaguchiRitsuko MatsumuraTakashi MatsuzakiWataru NakamuraKoichi NodeMakoto AkashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ai Yamaguchi Ritsuko Matsumura Takashi Matsuzaki Wataru Nakamura Koichi Node Makoto Akashi A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans
description	Abstract Almost all organisms maintain a circadian clock from birth to death to synchronize their own physiology and behavior with the earth’s rotation. Because the in vivo evaluation of human circadian characteristics is labor-intensive, in vitro or ex vivo approaches could provide advantages. In this study, to enable the simple and non-invasive evaluation of autonomous circadian oscillation, we established a method for monitoring clock gene expression by performing ex vivo culture of whole hair root tissue. This method is extremely simple and imposes little burden on subjects. Results obtained using Cryptochrome-deficient mice support that circadian period length in hair tissue correlates with intrinsic period length observed in physiology and behavior. We then applied this method to old-old subjects with severe dementia, who showed abnormal circadian behavior, and found that their peripheral clocks autonomously oscillated in a manner similar to those of healthy or younger subjects, indicating that the effect of cellular senescence on the autonomous clock oscillator is limited at least in some cell types. Although further validation may be required, the hair tissue-based culture assay would be a tool to investigate intrinsic circadian characteristics in humans.
format	article
author	Ai Yamaguchi Ritsuko Matsumura Takashi Matsuzaki Wataru Nakamura Koichi Node Makoto Akashi
author_facet	Ai Yamaguchi Ritsuko Matsumura Takashi Matsuzaki Wataru Nakamura Koichi Node Makoto Akashi
author_sort	Ai Yamaguchi
title	A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans
title_short	A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans
title_full	A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans
title_fullStr	A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans
title_full_unstemmed	A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans
title_sort	simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5aa270178b3640fe95051c018f962e05
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A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans

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