Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production

Abstract. Indah AWN, Rohmatussolihat, Rahayu WP, Setyoningrum F, Priadi G, Afianti F. 2020. Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production. Biodiversitas 21: 5403-5407. Deficiency of ?-galactosidase enzyme causes lactose to become undigested in...

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Autores principales:	Aafiyah Wahyu Nur Indah -, ROHMATUSSOLIHAT ROHMATUSSOLIHAT, WINIATI PUDJI RAHAYU, FITRI SETYONINGRUM, GUNAWAN PRIADI, FIFI AFIANTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	β-galactosidase, l.brevis lipi13-2-lab131, lactose, optimization, rsm Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5aa2fc777c6f4115a3b092db959fcdcf
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spelling	oai:doaj.org-article:5aa2fc777c6f4115a3b092db959fcdcf2021-11-22T00:44:11ZOptimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production1412-033X2085-472210.13057/biodiv/d211147https://doaj.org/article/5aa2fc777c6f4115a3b092db959fcdcf2020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4555https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Indah AWN, Rohmatussolihat, Rahayu WP, Setyoningrum F, Priadi G, Afianti F. 2020. Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production. Biodiversitas 21: 5403-5407. Deficiency of ?-galactosidase enzyme causes lactose to become undigested in gastrointestinal system, therefore the system needs further addition of external ?-galactosidase. The sources ?-galactosidase enzyme varies from plants, animals, and microorganisms. In industrial applications, microorganisms have become a considered potential source of ?-galactosidase. Lactobacillus brevis LIPI13-2-LAB131 had high ?-galactosidase enzyme activity, which was 7.93 U/mL. The aim of this research was to optimize the growth condition of L. brevis LIPI13-2-LAB131 in order to produce maximum ?-galactosidase enzyme activity. This research consisted of performing optimization processes using design expert 7.0 (DX7) program with response surface methodology (RSM) and partial purification of ?-galactosidase enzyme. The results of this research showed that the optimum growth conditions of L. brevis LIPI13-2-LAB131 were in 1.48% lactose level, incubation temperature of 34.91 °C, incubation time of 48.48 hours, and 2.83% inoculum size with desirability value of 0.839. The result of enzyme purification showed that value of ?-galactosidase enzyme activity increased up to 22.88 ± 0.29 U/mL with purification yield of 11.65%.Aafiyah Wahyu Nur Indah -ROHMATUSSOLIHAT ROHMATUSSOLIHATWINIATI PUDJI RAHAYUFITRI SETYONINGRUMGUNAWAN PRIADIFIFI AFIANTIMBI & UNS Soloarticleβ-galactosidase, l.brevis lipi13-2-lab131, lactose, optimization, rsmBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	β-galactosidase, l.brevis lipi13-2-lab131, lactose, optimization, rsm Biology (General) QH301-705.5
spellingShingle	β-galactosidase, l.brevis lipi13-2-lab131, lactose, optimization, rsm Biology (General) QH301-705.5 Aafiyah Wahyu Nur Indah - ROHMATUSSOLIHAT ROHMATUSSOLIHAT WINIATI PUDJI RAHAYU FITRI SETYONINGRUM GUNAWAN PRIADI FIFI AFIANTI Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production
description	Abstract. Indah AWN, Rohmatussolihat, Rahayu WP, Setyoningrum F, Priadi G, Afianti F. 2020. Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production. Biodiversitas 21: 5403-5407. Deficiency of ?-galactosidase enzyme causes lactose to become undigested in gastrointestinal system, therefore the system needs further addition of external ?-galactosidase. The sources ?-galactosidase enzyme varies from plants, animals, and microorganisms. In industrial applications, microorganisms have become a considered potential source of ?-galactosidase. Lactobacillus brevis LIPI13-2-LAB131 had high ?-galactosidase enzyme activity, which was 7.93 U/mL. The aim of this research was to optimize the growth condition of L. brevis LIPI13-2-LAB131 in order to produce maximum ?-galactosidase enzyme activity. This research consisted of performing optimization processes using design expert 7.0 (DX7) program with response surface methodology (RSM) and partial purification of ?-galactosidase enzyme. The results of this research showed that the optimum growth conditions of L. brevis LIPI13-2-LAB131 were in 1.48% lactose level, incubation temperature of 34.91 °C, incubation time of 48.48 hours, and 2.83% inoculum size with desirability value of 0.839. The result of enzyme purification showed that value of ?-galactosidase enzyme activity increased up to 22.88 ± 0.29 U/mL with purification yield of 11.65%.
format	article
author	Aafiyah Wahyu Nur Indah - ROHMATUSSOLIHAT ROHMATUSSOLIHAT WINIATI PUDJI RAHAYU FITRI SETYONINGRUM GUNAWAN PRIADI FIFI AFIANTI
author_facet	Aafiyah Wahyu Nur Indah - ROHMATUSSOLIHAT ROHMATUSSOLIHAT WINIATI PUDJI RAHAYU FITRI SETYONINGRUM GUNAWAN PRIADI FIFI AFIANTI
author_sort	Aafiyah Wahyu Nur Indah -
title	Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production
title_short	Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production
title_full	Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production
title_fullStr	Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production
title_full_unstemmed	Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production
title_sort	optimum growth conditions of lactobacillus brevis lipi13-2-lab131 in ?-galactosidase enzyme production
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/5aa2fc777c6f4115a3b092db959fcdcf
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Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in ?-galactosidase enzyme production

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