Biofilm Forming Ability Of Salmonella Enteritidis In Vitro

Salmonella enterica serotype Enteritidis is an important alimentary pathogen that recently gained special attention due to the ability of a large number of strains to form biofilms. Qualitative testing of biofilm forming ability was performed by observing the morphotype of the colonies on Congo Red...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ivana Čabarkapa, Marija Škrinjar, Jovanka Lević, Bojana Kokić, Nevena Blagojev, Dubravka Milanov, Ljiljana Suvajdžić
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2015
Materias:	salmonella enteritidis biofilm curli fimbriae cellulose Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/5ab6107b47904df8ae7b2c233c8d81f1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5ab6107b47904df8ae7b2c233c8d81f1
record_format	dspace
spelling	oai:doaj.org-article:5ab6107b47904df8ae7b2c233c8d81f12021-11-17T21:27:50ZBiofilm Forming Ability Of Salmonella Enteritidis In Vitro1820-744810.1515/acve-2015-0031https://doaj.org/article/5ab6107b47904df8ae7b2c233c8d81f12015-09-01T00:00:00Zhttps://doi.org/10.1515/acve-2015-0031https://doaj.org/toc/1820-7448Salmonella enterica serotype Enteritidis is an important alimentary pathogen that recently gained special attention due to the ability of a large number of strains to form biofilms. Qualitative testing of biofilm forming ability was performed by observing the morphotype of the colonies on Congo Red agar and by conducting the pellicle test, while quantitative testing was carried out by Cristal violet assay on microtiter plates. A total of 14 isolates of S. Enteritidis were tested for biofilm forming ability, while Salmonella Enteritidis ATTC 13076 was used as the reference strain. Based on the morphotype of colonies cultivated on Congo Red agar at 25°C incubation temperature, among tested isolates three morphotypes were detected – red, dry and rough (rdar), brown, dry and rough (bdar) and smooth and white (saw). Half of the tested isolates demonstrated rdar morphotype. All isolates that showed a specific morphotype at this incubation temperature also formed the corresponding type of pellicle at the air-liquid interface. Additionally, comparing OD (optical density) values obtained by crystal violet test between groups of isolates that represent one of the three detected morphotypes (rdar, bdar and saw), statistically significant differences were detected. Based on OD values obtained by crystal violet test at both applied incubation temperatures, isolates were classified into three categories, regarding their ability to form biofilms: strong, moderate and weak biofilm producers. By comparing the amounts of the biofilms formed after 48h at 25°C and 37°C, statistically significant differences were noted (P<0.05). In this research we presented micrographs and a reconstruction of three-dimensional projections of biofilm developing phases of rdar morphotype isolates, which were obtained using confocal laser scanning microscopy.Ivana ČabarkapaMarija ŠkrinjarJovanka LevićBojana KokićNevena BlagojevDubravka MilanovLjiljana SuvajdžićSciendoarticlesalmonella enteritidisbiofilmcurli fimbriaecelluloseVeterinary medicineSF600-1100ENActa Veterinaria, Vol 65, Iss 3, Pp 371-389 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	salmonella enteritidis biofilm curli fimbriae cellulose Veterinary medicine SF600-1100
spellingShingle	salmonella enteritidis biofilm curli fimbriae cellulose Veterinary medicine SF600-1100 Ivana Čabarkapa Marija Škrinjar Jovanka Lević Bojana Kokić Nevena Blagojev Dubravka Milanov Ljiljana Suvajdžić Biofilm Forming Ability Of Salmonella Enteritidis In Vitro
description	Salmonella enterica serotype Enteritidis is an important alimentary pathogen that recently gained special attention due to the ability of a large number of strains to form biofilms. Qualitative testing of biofilm forming ability was performed by observing the morphotype of the colonies on Congo Red agar and by conducting the pellicle test, while quantitative testing was carried out by Cristal violet assay on microtiter plates. A total of 14 isolates of S. Enteritidis were tested for biofilm forming ability, while Salmonella Enteritidis ATTC 13076 was used as the reference strain. Based on the morphotype of colonies cultivated on Congo Red agar at 25°C incubation temperature, among tested isolates three morphotypes were detected – red, dry and rough (rdar), brown, dry and rough (bdar) and smooth and white (saw). Half of the tested isolates demonstrated rdar morphotype. All isolates that showed a specific morphotype at this incubation temperature also formed the corresponding type of pellicle at the air-liquid interface. Additionally, comparing OD (optical density) values obtained by crystal violet test between groups of isolates that represent one of the three detected morphotypes (rdar, bdar and saw), statistically significant differences were detected. Based on OD values obtained by crystal violet test at both applied incubation temperatures, isolates were classified into three categories, regarding their ability to form biofilms: strong, moderate and weak biofilm producers. By comparing the amounts of the biofilms formed after 48h at 25°C and 37°C, statistically significant differences were noted (P<0.05). In this research we presented micrographs and a reconstruction of three-dimensional projections of biofilm developing phases of rdar morphotype isolates, which were obtained using confocal laser scanning microscopy.
format	article
author	Ivana Čabarkapa Marija Škrinjar Jovanka Lević Bojana Kokić Nevena Blagojev Dubravka Milanov Ljiljana Suvajdžić
author_facet	Ivana Čabarkapa Marija Škrinjar Jovanka Lević Bojana Kokić Nevena Blagojev Dubravka Milanov Ljiljana Suvajdžić
author_sort	Ivana Čabarkapa
title	Biofilm Forming Ability Of Salmonella Enteritidis In Vitro
title_short	Biofilm Forming Ability Of Salmonella Enteritidis In Vitro
title_full	Biofilm Forming Ability Of Salmonella Enteritidis In Vitro
title_fullStr	Biofilm Forming Ability Of Salmonella Enteritidis In Vitro
title_full_unstemmed	Biofilm Forming Ability Of Salmonella Enteritidis In Vitro
title_sort	biofilm forming ability of salmonella enteritidis in vitro
publisher	Sciendo
publishDate	2015
url	https://doaj.org/article/5ab6107b47904df8ae7b2c233c8d81f1
work_keys_str_mv	AT ivanacabarkapa biofilmformingabilityofsalmonellaenteritidisinvitro AT marijaskrinjar biofilmformingabilityofsalmonellaenteritidisinvitro AT jovankalevic biofilmformingabilityofsalmonellaenteritidisinvitro AT bojanakokic biofilmformingabilityofsalmonellaenteritidisinvitro AT nevenablagojev biofilmformingabilityofsalmonellaenteritidisinvitro AT dubravkamilanov biofilmformingabilityofsalmonellaenteritidisinvitro AT ljiljanasuvajdzic biofilmformingabilityofsalmonellaenteritidisinvitro
_version_	1718425357050183680

Biofilm Forming Ability Of Salmonella Enteritidis In Vitro

Ejemplares similares