Homeostasis of mucosal glial cells in human gut is independent of microbiota

Abstract In mammals, neural crest cells populate the gut and form the enteric nervous system (ENS) early in embryogenesis. Although the basic ENS structure is highly conserved across species, we show important differences between mice and humans relating to the prenatal and postnatal development of...

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Autores principales:	Timna Inlender, Einat Nissim-Eliraz, Rhian Stavely, Ryo Hotta, Allan M. Goldstein, Simcha Yagel, Michael J. Gutnick, Nahum Y. Shpigel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5abeb2169b57401aa332f2c1e53eb7db
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spelling	oai:doaj.org-article:5abeb2169b57401aa332f2c1e53eb7db2021-12-02T17:40:45ZHomeostasis of mucosal glial cells in human gut is independent of microbiota10.1038/s41598-021-92384-92045-2322https://doaj.org/article/5abeb2169b57401aa332f2c1e53eb7db2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92384-9https://doaj.org/toc/2045-2322Abstract In mammals, neural crest cells populate the gut and form the enteric nervous system (ENS) early in embryogenesis. Although the basic ENS structure is highly conserved across species, we show important differences between mice and humans relating to the prenatal and postnatal development of mucosal enteric glial cells (mEGC), which are essential ENS components. We confirm previous work showing that in the mouse mEGCs are absent at birth, and that their appearance and homeostasis depends on postnatal colonization by microbiota. In humans, by contrast, a network of glial cells is already present in the fetal gut. Moreover, in xenografts of human fetal gut maintained for months in immuno-compromised mice, mEGCs persist following treatment with antibiotics that lead to the disappearance of mEGCs from the gut of the murine host. Single cell RNAseq indicates that human and mouse mEGCs differ not only in their developmental dynamics, but also in their patterns of gene expression.Timna InlenderEinat Nissim-ElirazRhian StavelyRyo HottaAllan M. GoldsteinSimcha YagelMichael J. GutnickNahum Y. ShpigelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Timna Inlender Einat Nissim-Eliraz Rhian Stavely Ryo Hotta Allan M. Goldstein Simcha Yagel Michael J. Gutnick Nahum Y. Shpigel Homeostasis of mucosal glial cells in human gut is independent of microbiota
description	Abstract In mammals, neural crest cells populate the gut and form the enteric nervous system (ENS) early in embryogenesis. Although the basic ENS structure is highly conserved across species, we show important differences between mice and humans relating to the prenatal and postnatal development of mucosal enteric glial cells (mEGC), which are essential ENS components. We confirm previous work showing that in the mouse mEGCs are absent at birth, and that their appearance and homeostasis depends on postnatal colonization by microbiota. In humans, by contrast, a network of glial cells is already present in the fetal gut. Moreover, in xenografts of human fetal gut maintained for months in immuno-compromised mice, mEGCs persist following treatment with antibiotics that lead to the disappearance of mEGCs from the gut of the murine host. Single cell RNAseq indicates that human and mouse mEGCs differ not only in their developmental dynamics, but also in their patterns of gene expression.
format	article
author	Timna Inlender Einat Nissim-Eliraz Rhian Stavely Ryo Hotta Allan M. Goldstein Simcha Yagel Michael J. Gutnick Nahum Y. Shpigel
author_facet	Timna Inlender Einat Nissim-Eliraz Rhian Stavely Ryo Hotta Allan M. Goldstein Simcha Yagel Michael J. Gutnick Nahum Y. Shpigel
author_sort	Timna Inlender
title	Homeostasis of mucosal glial cells in human gut is independent of microbiota
title_short	Homeostasis of mucosal glial cells in human gut is independent of microbiota
title_full	Homeostasis of mucosal glial cells in human gut is independent of microbiota
title_fullStr	Homeostasis of mucosal glial cells in human gut is independent of microbiota
title_full_unstemmed	Homeostasis of mucosal glial cells in human gut is independent of microbiota
title_sort	homeostasis of mucosal glial cells in human gut is independent of microbiota
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5abeb2169b57401aa332f2c1e53eb7db
work_keys_str_mv	AT timnainlender homeostasisofmucosalglialcellsinhumangutisindependentofmicrobiota AT einatnissimeliraz homeostasisofmucosalglialcellsinhumangutisindependentofmicrobiota AT rhianstavely homeostasisofmucosalglialcellsinhumangutisindependentofmicrobiota AT ryohotta homeostasisofmucosalglialcellsinhumangutisindependentofmicrobiota AT allanmgoldstein homeostasisofmucosalglialcellsinhumangutisindependentofmicrobiota AT simchayagel homeostasisofmucosalglialcellsinhumangutisindependentofmicrobiota AT michaeljgutnick homeostasisofmucosalglialcellsinhumangutisindependentofmicrobiota AT nahumyshpigel homeostasisofmucosalglialcellsinhumangutisindependentofmicrobiota
_version_	1718379764830437376

Homeostasis of mucosal glial cells in human gut is independent of microbiota

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