A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response

Abstract Type-III secreted effectors (T3Es) play critical roles during bacterial pathogenesis in plants. Plant recognition of certain T3Es can trigger defence, often accompanied by macroscopic cell death, termed the hypersensitive response (HR). Economically important species of kiwifruit are suscep...

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Autores principales:	Jay Jayaraman, Sera Choi, Maxim Prokchorchik, Du Seok Choi, Amandine Spiandore, Erik H. Rikkerink, Matthew D. Templeton, Cécile Segonzac, Kee Hoon Sohn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ac4eb15925e4d5587b1169e9d222621
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spelling	oai:doaj.org-article:5ac4eb15925e4d5587b1169e9d2226212021-12-02T12:32:50ZA bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response10.1038/s41598-017-03704-x2045-2322https://doaj.org/article/5ac4eb15925e4d5587b1169e9d2226212017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03704-xhttps://doaj.org/toc/2045-2322Abstract Type-III secreted effectors (T3Es) play critical roles during bacterial pathogenesis in plants. Plant recognition of certain T3Es can trigger defence, often accompanied by macroscopic cell death, termed the hypersensitive response (HR). Economically important species of kiwifruit are susceptible to Pseudomonas syringae pv. actinidiae (Psa), the causal agent of kiwifruit bacterial canker. Although Psa is non-pathogenic in Arabidopsis thaliana, we observed that a T3E, HopZ5 that is unique to a global outbreak clade of Psa, triggers HR and defence in Arabidopsis accession Ct-1. Ws-2 and Col-0 accessions are unable to produce an HR in response to Pseudomonas-delivered HopZ5. While Ws-2 is susceptible to virulent bacterial strain Pseudomonas syringae pv. tomato DC3000 carrying HopZ5, Col-0 is resistant despite the lack of an HR. We show that HopZ5, like other members of the YopJ superfamily of acetyltransferases that it belongs to, autoacetylates lysine residues. Through comparisons to other family members, we identified an acetyltransferase catalytic activity and demonstrate its requirement for triggering defence in Arabidopsis and Nicotiana species. Collectively, data herein indicate that HopZ5 is a plasma membrane-localized acetyltransferase with autoacetylation activity required for avirulence.Jay JayaramanSera ChoiMaxim ProkchorchikDu Seok ChoiAmandine SpiandoreErik H. RikkerinkMatthew D. TempletonCécile SegonzacKee Hoon SohnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jay Jayaraman Sera Choi Maxim Prokchorchik Du Seok Choi Amandine Spiandore Erik H. Rikkerink Matthew D. Templeton Cécile Segonzac Kee Hoon Sohn A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response
description	Abstract Type-III secreted effectors (T3Es) play critical roles during bacterial pathogenesis in plants. Plant recognition of certain T3Es can trigger defence, often accompanied by macroscopic cell death, termed the hypersensitive response (HR). Economically important species of kiwifruit are susceptible to Pseudomonas syringae pv. actinidiae (Psa), the causal agent of kiwifruit bacterial canker. Although Psa is non-pathogenic in Arabidopsis thaliana, we observed that a T3E, HopZ5 that is unique to a global outbreak clade of Psa, triggers HR and defence in Arabidopsis accession Ct-1. Ws-2 and Col-0 accessions are unable to produce an HR in response to Pseudomonas-delivered HopZ5. While Ws-2 is susceptible to virulent bacterial strain Pseudomonas syringae pv. tomato DC3000 carrying HopZ5, Col-0 is resistant despite the lack of an HR. We show that HopZ5, like other members of the YopJ superfamily of acetyltransferases that it belongs to, autoacetylates lysine residues. Through comparisons to other family members, we identified an acetyltransferase catalytic activity and demonstrate its requirement for triggering defence in Arabidopsis and Nicotiana species. Collectively, data herein indicate that HopZ5 is a plasma membrane-localized acetyltransferase with autoacetylation activity required for avirulence.
format	article
author	Jay Jayaraman Sera Choi Maxim Prokchorchik Du Seok Choi Amandine Spiandore Erik H. Rikkerink Matthew D. Templeton Cécile Segonzac Kee Hoon Sohn
author_facet	Jay Jayaraman Sera Choi Maxim Prokchorchik Du Seok Choi Amandine Spiandore Erik H. Rikkerink Matthew D. Templeton Cécile Segonzac Kee Hoon Sohn
author_sort	Jay Jayaraman
title	A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response
title_short	A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response
title_full	A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response
title_fullStr	A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response
title_full_unstemmed	A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response
title_sort	bacterial acetyltransferase triggers immunity in arabidopsis thaliana independent of hypersensitive response
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5ac4eb15925e4d5587b1169e9d222621
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A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response

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