Red blood cells are dynamic reservoirs of cytokines

Abstract Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous s...

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Autores principales:	Elisabeth Karsten, Edmond Breen, Benjamin R. Herbert
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5acbcd7793124a8885b94322e9a0f096
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spelling	oai:doaj.org-article:5acbcd7793124a8885b94322e9a0f0962021-12-02T15:08:30ZRed blood cells are dynamic reservoirs of cytokines10.1038/s41598-018-21387-w2045-2322https://doaj.org/article/5acbcd7793124a8885b94322e9a0f0962018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21387-whttps://doaj.org/toc/2045-2322Abstract Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex® technology. This analysis revealed that, after correcting for white blood cell and platelet contamination, 46 cytokines were detected in RBC lysates, and the median concentration in RBCs was 12-fold higher than in the plasma. In addition, extensive washing of RBCs, such as that performed in proteomics analyses or prior to some RBC transfusions, significantly attenuated the release of six cytokines following incubation at 37 °C. This supports the hypothesis that, alongside its gas exchange function, RBCs play a role in cytokine signalling. This discovery may help supplement disease biomarker research and may shed light on adverse inflammatory processes that can follow RBC transfusion.Elisabeth KarstenEdmond BreenBenjamin R. HerbertNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elisabeth Karsten Edmond Breen Benjamin R. Herbert Red blood cells are dynamic reservoirs of cytokines
description	Abstract Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex® technology. This analysis revealed that, after correcting for white blood cell and platelet contamination, 46 cytokines were detected in RBC lysates, and the median concentration in RBCs was 12-fold higher than in the plasma. In addition, extensive washing of RBCs, such as that performed in proteomics analyses or prior to some RBC transfusions, significantly attenuated the release of six cytokines following incubation at 37 °C. This supports the hypothesis that, alongside its gas exchange function, RBCs play a role in cytokine signalling. This discovery may help supplement disease biomarker research and may shed light on adverse inflammatory processes that can follow RBC transfusion.
format	article
author	Elisabeth Karsten Edmond Breen Benjamin R. Herbert
author_facet	Elisabeth Karsten Edmond Breen Benjamin R. Herbert
author_sort	Elisabeth Karsten
title	Red blood cells are dynamic reservoirs of cytokines
title_short	Red blood cells are dynamic reservoirs of cytokines
title_full	Red blood cells are dynamic reservoirs of cytokines
title_fullStr	Red blood cells are dynamic reservoirs of cytokines
title_full_unstemmed	Red blood cells are dynamic reservoirs of cytokines
title_sort	red blood cells are dynamic reservoirs of cytokines
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5acbcd7793124a8885b94322e9a0f096
work_keys_str_mv	AT elisabethkarsten redbloodcellsaredynamicreservoirsofcytokines AT edmondbreen redbloodcellsaredynamicreservoirsofcytokines AT benjaminrherbert redbloodcellsaredynamicreservoirsofcytokines
_version_	1718388123164999680

Red blood cells are dynamic reservoirs of cytokines

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