Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans

Abstract To characterize the molecular mechanisms underlying life-stage transitions in Phytophthora infestans, we initiated a chemical genetics approach by screening for a stage-specific inhibitor of morphological development from microbial culture extracts prepared mostly from actinomycetes from so...

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Autores principales:	Shuji Tani, Naotaka Nishio, Kenji Kai, Daisuke Hagiwara, Yoshiyuki Ogata, Motoaki Tojo, Jun-ichi Sumitani, Howard S. Judelson, Takashi Kawaguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5acbe45a0c4745d78a44a96cc36b5511
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spelling	oai:doaj.org-article:5acbe45a0c4745d78a44a96cc36b55112021-12-02T12:42:28ZChemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans10.1038/s41598-020-79326-72045-2322https://doaj.org/article/5acbe45a0c4745d78a44a96cc36b55112020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79326-7https://doaj.org/toc/2045-2322Abstract To characterize the molecular mechanisms underlying life-stage transitions in Phytophthora infestans, we initiated a chemical genetics approach by screening for a stage-specific inhibitor of morphological development from microbial culture extracts prepared mostly from actinomycetes from soil in Japan. Of the more than 700 extracts, one consistently inhibited Ph. infestans cyst germination. Purification and identification of the active compound by ESI–MS, 1H-NMR, and 13C-NMR identified β-rubromycin as the inhibitor of cyst germination (IC50 = 19.8 μg/L); β-rubromycin did not inhibit growth on rye media, sporangium formation, zoospore release, cyst formation, or appressorium formation in Ph. infestans. Further analyses revealed that β-rubromycin inhibited the germination of cysts and oospores in Pythium aphanidermatum. A chemical genetic approach revealed that β-rubromycin stimulated the expression of RIO kinase-like gene (PITG_04584) by 60-fold in Ph. infestans. Genetic analyses revealed that PITG_04584, which lacks close non-oomycete relatives, was involved in zoosporogenesis, cyst germination, and appressorium formation in Ph. infestans. These data imply that further functional analyses of PITG_04584 may contribute to new methods to suppress diseases caused by oomycetes.Shuji TaniNaotaka NishioKenji KaiDaisuke HagiwaraYoshiyuki OgataMotoaki TojoJun-ichi SumitaniHoward S. JudelsonTakashi KawaguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuji Tani Naotaka Nishio Kenji Kai Daisuke Hagiwara Yoshiyuki Ogata Motoaki Tojo Jun-ichi Sumitani Howard S. Judelson Takashi Kawaguchi Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
description	Abstract To characterize the molecular mechanisms underlying life-stage transitions in Phytophthora infestans, we initiated a chemical genetics approach by screening for a stage-specific inhibitor of morphological development from microbial culture extracts prepared mostly from actinomycetes from soil in Japan. Of the more than 700 extracts, one consistently inhibited Ph. infestans cyst germination. Purification and identification of the active compound by ESI–MS, 1H-NMR, and 13C-NMR identified β-rubromycin as the inhibitor of cyst germination (IC50 = 19.8 μg/L); β-rubromycin did not inhibit growth on rye media, sporangium formation, zoospore release, cyst formation, or appressorium formation in Ph. infestans. Further analyses revealed that β-rubromycin inhibited the germination of cysts and oospores in Pythium aphanidermatum. A chemical genetic approach revealed that β-rubromycin stimulated the expression of RIO kinase-like gene (PITG_04584) by 60-fold in Ph. infestans. Genetic analyses revealed that PITG_04584, which lacks close non-oomycete relatives, was involved in zoosporogenesis, cyst germination, and appressorium formation in Ph. infestans. These data imply that further functional analyses of PITG_04584 may contribute to new methods to suppress diseases caused by oomycetes.
format	article
author	Shuji Tani Naotaka Nishio Kenji Kai Daisuke Hagiwara Yoshiyuki Ogata Motoaki Tojo Jun-ichi Sumitani Howard S. Judelson Takashi Kawaguchi
author_facet	Shuji Tani Naotaka Nishio Kenji Kai Daisuke Hagiwara Yoshiyuki Ogata Motoaki Tojo Jun-ichi Sumitani Howard S. Judelson Takashi Kawaguchi
author_sort	Shuji Tani
title	Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
title_short	Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
title_full	Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
title_fullStr	Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
title_full_unstemmed	Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
title_sort	chemical genetic approach using β-rubromycin reveals that a rio kinase-like protein is involved in morphological development in phytophthora infestans
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5acbe45a0c4745d78a44a96cc36b5511
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Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans

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