The Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans

Transient receptor potential (TRP) channel Yvc1 was related with hyphal growth, oxidative stress response, and pathogenicity. Calcineurin subunit Cnb1 was activated immediately in yeasts when exposed to severe stimulation. However, the relationship between Yvc1 and Cnb1-governed calcium ions and end...

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Autores principales:	Liping Peng, Jiawen Du, Runfan Zhang, Nali Zhu, He Zhao, Qiang Zhao, Qilin Yu, Mingchun Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	calcineurin TRP channel calcium transport endoplasmic reticulum stress Candida albicans Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5ad3c8187a544de686e7a871f02f3359
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spelling	oai:doaj.org-article:5ad3c8187a544de686e7a871f02f33592021-12-01T18:01:13ZThe Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans1664-302X10.3389/fmicb.2021.752670https://doaj.org/article/5ad3c8187a544de686e7a871f02f33592021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.752670/fullhttps://doaj.org/toc/1664-302XTransient receptor potential (TRP) channel Yvc1 was related with hyphal growth, oxidative stress response, and pathogenicity. Calcineurin subunit Cnb1 was activated immediately in yeasts when exposed to severe stimulation. However, the relationship between Yvc1 and Cnb1-governed calcium ions and endoplasmic reticulum (ER) stress response remains unrevealed. In this study, we found that the mutant cnb1Δ/Δ was sensitive to TN, which was related with the overexpression of membrane calcium ion channels that could increase the cytosol calcium concentration. However, the growth of the cnb1Δ/Δyvc1Δ/Δ mutant was recovered and its cell vitality was better than the cnb1Δ/Δ strain. Meanwhile, the cellular calcium concentration was decreased and its fluctuation was weakened under ER stress in the cnb1Δ/Δyvc1Δ/Δ strain. To verify the regulation role of Yvc1 in the calcium concentration, we found that the addition of CaCl2 led to the worse viability, while the growth state was relieved under the treatment of EGTA in the cnb1Δ/Δ strain. In conclusion, the deletion of YVC1 could reduce the cellular calcium and relieve the ER stress sensitivity of the cnb1Δ/Δ strain. Thereby, our findings shed a novel light on the relationship between the Yvc1-governed cellular calcium concentration and ER stress response in C. albicans.Liping PengJiawen DuRunfan ZhangNali ZhuHe ZhaoQiang ZhaoQilin YuMingchun LiFrontiers Media S.A.articlecalcineurinTRP channelcalcium transportendoplasmic reticulum stressCandida albicansMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	calcineurin TRP channel calcium transport endoplasmic reticulum stress Candida albicans Microbiology QR1-502
spellingShingle	calcineurin TRP channel calcium transport endoplasmic reticulum stress Candida albicans Microbiology QR1-502 Liping Peng Jiawen Du Runfan Zhang Nali Zhu He Zhao Qiang Zhao Qilin Yu Mingchun Li The Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans
description	Transient receptor potential (TRP) channel Yvc1 was related with hyphal growth, oxidative stress response, and pathogenicity. Calcineurin subunit Cnb1 was activated immediately in yeasts when exposed to severe stimulation. However, the relationship between Yvc1 and Cnb1-governed calcium ions and endoplasmic reticulum (ER) stress response remains unrevealed. In this study, we found that the mutant cnb1Δ/Δ was sensitive to TN, which was related with the overexpression of membrane calcium ion channels that could increase the cytosol calcium concentration. However, the growth of the cnb1Δ/Δyvc1Δ/Δ mutant was recovered and its cell vitality was better than the cnb1Δ/Δ strain. Meanwhile, the cellular calcium concentration was decreased and its fluctuation was weakened under ER stress in the cnb1Δ/Δyvc1Δ/Δ strain. To verify the regulation role of Yvc1 in the calcium concentration, we found that the addition of CaCl2 led to the worse viability, while the growth state was relieved under the treatment of EGTA in the cnb1Δ/Δ strain. In conclusion, the deletion of YVC1 could reduce the cellular calcium and relieve the ER stress sensitivity of the cnb1Δ/Δ strain. Thereby, our findings shed a novel light on the relationship between the Yvc1-governed cellular calcium concentration and ER stress response in C. albicans.
format	article
author	Liping Peng Jiawen Du Runfan Zhang Nali Zhu He Zhao Qiang Zhao Qilin Yu Mingchun Li
author_facet	Liping Peng Jiawen Du Runfan Zhang Nali Zhu He Zhao Qiang Zhao Qilin Yu Mingchun Li
author_sort	Liping Peng
title	The Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans
title_short	The Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans
title_full	The Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans
title_fullStr	The Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans
title_full_unstemmed	The Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans
title_sort	transient receptor potential channel yvc1 deletion recovers the growth defect of calcineurin mutant under endoplasmic reticulum stress in candida albicans
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5ad3c8187a544de686e7a871f02f3359
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The Transient Receptor Potential Channel Yvc1 Deletion Recovers the Growth Defect of Calcineurin Mutant Under Endoplasmic Reticulum Stress in Candida albicans

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