Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution

The tumor microenvironment heterogeneity of papillary thyroid cancer (PTC) is poorly characterized. The relationship between PTC and Hashimoto thyroiditis (HT) is also in doubt. Here, we used single-cell RNA sequencing to map the transcriptome landscape of PTC from eight PTC patients, of which three...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun Pan, Fang Ye, Chengxuan Yu, Qinsheng Zhu, Jiaqi Li, Yaohui Zhang, Hedi Tian, Yunjin Yao, Minjie Zhu, Yibin Shen, Feng Zhu, Yingying Wang, Xinhui Zhou, Guoji Guo, Yijun Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	papillary thyroid cancer Hashimoto’s thyroiditis single-cell RNA sequencing tumor B-cell infiltration immunological link Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5ad4eb7665fe453eb961cadabacc7640
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5ad4eb7665fe453eb961cadabacc7640
record_format	dspace
spelling	oai:doaj.org-article:5ad4eb7665fe453eb961cadabacc76402021-11-05T11:07:29ZPapillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution2296-634X10.3389/fcell.2021.758339https://doaj.org/article/5ad4eb7665fe453eb961cadabacc76402021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.758339/fullhttps://doaj.org/toc/2296-634XThe tumor microenvironment heterogeneity of papillary thyroid cancer (PTC) is poorly characterized. The relationship between PTC and Hashimoto thyroiditis (HT) is also in doubt. Here, we used single-cell RNA sequencing to map the transcriptome landscape of PTC from eight PTC patients, of which three were concurrent with HT. Predicted copy number variation in epithelial cells and mesenchymal cells revealed the distinct molecular signatures of carcinoma cells. Carcinoma cells demonstrated intertumoral heterogeneity based on BRAF V600E mutation or lymph node metastasis, and some altered genes were identified to be correlated with disease-free survival in The Cancer Genome Atlas datasets. In addition, transcription factor regulons of follicular epithelial cells unveil the different transcription activation state in PTC patients with or without concurrent HT. The immune cells in tumors exhibited distinct transcriptional states, and the presence of tumor-infiltrating B lymphocytes was predominantly linked to concurrent HT origin. Trajectory analysis of B cells and plasma cells suggested their migration potential from HT adjacent tissues to tumor tissues. Furthermore, we revealed diverse ligand–receptor pairs between non-immune cells, infiltrating myeloid cells, and lymphocytes. Our results provided a single-cell landscape of human PTC. These data would deepen the understanding of PTC, as well as the immunological link between PTC and HT.Jun PanFang YeChengxuan YuQinsheng ZhuJiaqi LiYaohui ZhangHedi TianYunjin YaoMinjie ZhuYibin ShenFeng ZhuYingying WangXinhui ZhouGuoji GuoGuoji GuoGuoji GuoGuoji GuoGuoji GuoYijun WuFrontiers Media S.A.articlepapillary thyroid cancerHashimoto’s thyroiditissingle-cell RNA sequencingtumor B-cell infiltrationimmunological linkBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	papillary thyroid cancer Hashimoto’s thyroiditis single-cell RNA sequencing tumor B-cell infiltration immunological link Biology (General) QH301-705.5
spellingShingle	papillary thyroid cancer Hashimoto’s thyroiditis single-cell RNA sequencing tumor B-cell infiltration immunological link Biology (General) QH301-705.5 Jun Pan Fang Ye Chengxuan Yu Qinsheng Zhu Jiaqi Li Yaohui Zhang Hedi Tian Yunjin Yao Minjie Zhu Yibin Shen Feng Zhu Yingying Wang Xinhui Zhou Guoji Guo Guoji Guo Guoji Guo Guoji Guo Guoji Guo Yijun Wu Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution
description	The tumor microenvironment heterogeneity of papillary thyroid cancer (PTC) is poorly characterized. The relationship between PTC and Hashimoto thyroiditis (HT) is also in doubt. Here, we used single-cell RNA sequencing to map the transcriptome landscape of PTC from eight PTC patients, of which three were concurrent with HT. Predicted copy number variation in epithelial cells and mesenchymal cells revealed the distinct molecular signatures of carcinoma cells. Carcinoma cells demonstrated intertumoral heterogeneity based on BRAF V600E mutation or lymph node metastasis, and some altered genes were identified to be correlated with disease-free survival in The Cancer Genome Atlas datasets. In addition, transcription factor regulons of follicular epithelial cells unveil the different transcription activation state in PTC patients with or without concurrent HT. The immune cells in tumors exhibited distinct transcriptional states, and the presence of tumor-infiltrating B lymphocytes was predominantly linked to concurrent HT origin. Trajectory analysis of B cells and plasma cells suggested their migration potential from HT adjacent tissues to tumor tissues. Furthermore, we revealed diverse ligand–receptor pairs between non-immune cells, infiltrating myeloid cells, and lymphocytes. Our results provided a single-cell landscape of human PTC. These data would deepen the understanding of PTC, as well as the immunological link between PTC and HT.
format	article
author	Jun Pan Fang Ye Chengxuan Yu Qinsheng Zhu Jiaqi Li Yaohui Zhang Hedi Tian Yunjin Yao Minjie Zhu Yibin Shen Feng Zhu Yingying Wang Xinhui Zhou Guoji Guo Guoji Guo Guoji Guo Guoji Guo Guoji Guo Yijun Wu
author_facet	Jun Pan Fang Ye Chengxuan Yu Qinsheng Zhu Jiaqi Li Yaohui Zhang Hedi Tian Yunjin Yao Minjie Zhu Yibin Shen Feng Zhu Yingying Wang Xinhui Zhou Guoji Guo Guoji Guo Guoji Guo Guoji Guo Guoji Guo Yijun Wu
author_sort	Jun Pan
title	Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution
title_short	Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution
title_full	Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution
title_fullStr	Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution
title_full_unstemmed	Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution
title_sort	papillary thyroid carcinoma landscape and its immunological link with hashimoto thyroiditis at single-cell resolution
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5ad4eb7665fe453eb961cadabacc7640
work_keys_str_mv	AT junpan papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT fangye papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT chengxuanyu papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT qinshengzhu papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT jiaqili papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT yaohuizhang papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT heditian papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT yunjinyao papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT minjiezhu papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT yibinshen papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT fengzhu papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT yingyingwang papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT xinhuizhou papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT guojiguo papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT guojiguo papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT guojiguo papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT guojiguo papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT guojiguo papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution AT yijunwu papillarythyroidcarcinomalandscapeanditsimmunologicallinkwithhashimotothyroiditisatsinglecellresolution
_version_	1718444291441819648

Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution

Ejemplares similares