Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy

Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy

Luciana LC Estevanato,1 Jaqueline R Da Silva,1 André M Falqueiro,2 Ewa Mosiniewicz-Szablewska,3 Piotr Suchocki,4,5 Antônio C Tedesco,2 Paulo C Morais,6 Zulmira GM Lacava11Instituto de Ciências Biológicas, Universidade de Brasíl...

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Autores principales: Estevanato LC, Da Silva JR, Falqueiro AM, Mosiniewicz-Szablewska E, Suchocki P, Tedesco AC, Morais PC, Lacava ZG
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
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spelling oai:doaj.org-article:5ad65dbdb922482796c7979ee55ef2ca2021-12-02T01:50:39ZCo-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy1176-91141178-2013https://doaj.org/article/5ad65dbdb922482796c7979ee55ef2ca2012-10-01T00:00:00Zhttp://www.dovepress.com/co-nanoencapsulation-of-magnetic-nanoparticles-and-selol-for-breast-tu-a11196https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Luciana LC Estevanato,1 Jaqueline R Da Silva,1 André M Falqueiro,2 Ewa Mosiniewicz-Szablewska,3 Piotr Suchocki,4,5 Antônio C Tedesco,2 Paulo C Morais,6 Zulmira GM Lacava11Instituto de Ciências Biológicas, Universidade de Brasília, Brasília DF, Brazil; 2Departamento de Química, Laboratório de Fotobiologia e Fotomedicina, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP, Brazil; 3Institute of Physics, Polish Academy of Sciences, Warsaw, Poland; 4Department of Drug Analysis, Warsaw Medical University, Warsaw, Poland; 5Department of Pharmaceutical Chemistry, National Medicines Institute, Warsaw, Poland; 6Instituto de Física, Universidade de Brasília, Brasília DF, BrazilAbstract: Antitumor activities have been described in selol, a hydrophobic mixture of molecules containing selenium in their structure, and also in maghemite magnetic nanoparticles (MNPs). Both selol and MNPs were co-encapsulated within poly(lactic-co-glycolic acid) (PLGA) nanocapsules for therapeutic purposes. The PLGA-nanocapsules loaded with MNPs and selol were labeled MSE-NC and characterized by transmission and scanning electron microscopy, electrophoretic mobility, photon correlation spectroscopy, presenting a monodisperse profile, and positive charge. The antitumor effect of MSE-NC was evaluated using normal (MCF-10A) and neoplastic (4T1 and MCF-7) breast cell lines. Nanocapsules containing only MNPs or selol were used as control. MTT assay showed that the cytotoxicity induced by MSE-NC was dose and time dependent. Normal cells were less affected than tumor cells. Cell death occurred mainly by apoptosis. Further exposure of MSE-NC treated neoplastic breast cells to an alternating magnetic field increased the antitumor effect of MSE-NC. It was concluded that selol-loaded magnetic PLGA-nanocapsules (MSE-NC) represent an effective magnetic material platform to promote magnetohyperthermia and thus a potential system for antitumor therapy.Keywords: PLGA-nanocapsule, cancer, maghemite nanoparticle, MTT, drug delivery, seleniumEstevanato LCDa Silva JRFalqueiro AMMosiniewicz-Szablewska ESuchocki PTedesco ACMorais PCLacava ZGDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5287-5299 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Estevanato LC
Da Silva JR
Falqueiro AM
Mosiniewicz-Szablewska E
Suchocki P
Tedesco AC
Morais PC
Lacava ZG
Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy
description Luciana LC Estevanato,1 Jaqueline R Da Silva,1 André M Falqueiro,2 Ewa Mosiniewicz-Szablewska,3 Piotr Suchocki,4,5 Antônio C Tedesco,2 Paulo C Morais,6 Zulmira GM Lacava11Instituto de Ciências Biológicas, Universidade de Brasília, Brasília DF, Brazil; 2Departamento de Química, Laboratório de Fotobiologia e Fotomedicina, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP, Brazil; 3Institute of Physics, Polish Academy of Sciences, Warsaw, Poland; 4Department of Drug Analysis, Warsaw Medical University, Warsaw, Poland; 5Department of Pharmaceutical Chemistry, National Medicines Institute, Warsaw, Poland; 6Instituto de Física, Universidade de Brasília, Brasília DF, BrazilAbstract: Antitumor activities have been described in selol, a hydrophobic mixture of molecules containing selenium in their structure, and also in maghemite magnetic nanoparticles (MNPs). Both selol and MNPs were co-encapsulated within poly(lactic-co-glycolic acid) (PLGA) nanocapsules for therapeutic purposes. The PLGA-nanocapsules loaded with MNPs and selol were labeled MSE-NC and characterized by transmission and scanning electron microscopy, electrophoretic mobility, photon correlation spectroscopy, presenting a monodisperse profile, and positive charge. The antitumor effect of MSE-NC was evaluated using normal (MCF-10A) and neoplastic (4T1 and MCF-7) breast cell lines. Nanocapsules containing only MNPs or selol were used as control. MTT assay showed that the cytotoxicity induced by MSE-NC was dose and time dependent. Normal cells were less affected than tumor cells. Cell death occurred mainly by apoptosis. Further exposure of MSE-NC treated neoplastic breast cells to an alternating magnetic field increased the antitumor effect of MSE-NC. It was concluded that selol-loaded magnetic PLGA-nanocapsules (MSE-NC) represent an effective magnetic material platform to promote magnetohyperthermia and thus a potential system for antitumor therapy.Keywords: PLGA-nanocapsule, cancer, maghemite nanoparticle, MTT, drug delivery, selenium
format article
author Estevanato LC
Da Silva JR
Falqueiro AM
Mosiniewicz-Szablewska E
Suchocki P
Tedesco AC
Morais PC
Lacava ZG
author_facet Estevanato LC
Da Silva JR
Falqueiro AM
Mosiniewicz-Szablewska E
Suchocki P
Tedesco AC
Morais PC
Lacava ZG
author_sort Estevanato LC
title Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy
title_short Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy
title_full Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy
title_fullStr Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy
title_full_unstemmed Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy
title_sort co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/5ad65dbdb922482796c7979ee55ef2ca
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