Effect of illumination level [18F]FDG-PET brain uptake in free moving mice.

In both clinical and preclinical scenarios, 2-deoxy-2[18F]fluoro-D-glucose ([18F]FDG) is the radiotracer most widely used to study brain glucose metabolism with positron emission tomography (PET). In clinical practice, there is a worldwide standardized protocol for preparing patients for [18F]FDG-PE...

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Autores principales:	Alexandra de Francisco, Yolanda Sierra-Palomares, María Felipe, Daniel Calle, Manuel Desco, Lorena Cussó
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ad98df99f3546d1ad24d448fee8d5a9
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id	oai:doaj.org-article:5ad98df99f3546d1ad24d448fee8d5a9
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spelling	oai:doaj.org-article:5ad98df99f3546d1ad24d448fee8d5a92021-12-02T20:04:06ZEffect of illumination level [18F]FDG-PET brain uptake in free moving mice.1932-620310.1371/journal.pone.0251454https://doaj.org/article/5ad98df99f3546d1ad24d448fee8d5a92021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251454https://doaj.org/toc/1932-6203In both clinical and preclinical scenarios, 2-deoxy-2[18F]fluoro-D-glucose ([18F]FDG) is the radiotracer most widely used to study brain glucose metabolism with positron emission tomography (PET). In clinical practice, there is a worldwide standardized protocol for preparing patients for [18F]FDG-PET studies, which specifies the room lighting. However, this standard is typically not observed in the preclinical field, although it is well known that animal handling affects the biodistribution of [18F]FDG. The present study aimed to evaluate the effect of ambient lighting on brain [18F]FDG uptake in mice. Two [18F]FDG-PET studies were performed on each animal, one in light and one in dark conditions. Thermal video recordings were acquired to analyse animal motor activity in both conditions. [18F]FDG-PET images were analysed with the Statistical Parametric Mapping method. The results showed that [18F]FDG uptake is higher in darkness than in light condition in mouse nucleus accumbens, hippocampus, midbrain, hindbrain, and cerebellum. The SPM analysis also showed an interaction between the illumination condition and the sex of the animal. Mouse activity was significantly different (p = 0.01) between light conditions (632 ± 215 s of movement) and dark conditions (989 ± 200 s), without significant effect of sex (p = 0.416). We concluded that room illumination conditions during [18F]FDG uptake in mice affected the brain [18F]FDG biodistribution. Therefore, we highlight the importance to control this factor to ensure more reliable and reproducible mouse brain [18F]FDG-PET results.Alexandra de FranciscoYolanda Sierra-PalomaresMaría FelipeDaniel CalleManuel DescoLorena CussóPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251454 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandra de Francisco Yolanda Sierra-Palomares María Felipe Daniel Calle Manuel Desco Lorena Cussó Effect of illumination level [18F]FDG-PET brain uptake in free moving mice.
description	In both clinical and preclinical scenarios, 2-deoxy-2[18F]fluoro-D-glucose ([18F]FDG) is the radiotracer most widely used to study brain glucose metabolism with positron emission tomography (PET). In clinical practice, there is a worldwide standardized protocol for preparing patients for [18F]FDG-PET studies, which specifies the room lighting. However, this standard is typically not observed in the preclinical field, although it is well known that animal handling affects the biodistribution of [18F]FDG. The present study aimed to evaluate the effect of ambient lighting on brain [18F]FDG uptake in mice. Two [18F]FDG-PET studies were performed on each animal, one in light and one in dark conditions. Thermal video recordings were acquired to analyse animal motor activity in both conditions. [18F]FDG-PET images were analysed with the Statistical Parametric Mapping method. The results showed that [18F]FDG uptake is higher in darkness than in light condition in mouse nucleus accumbens, hippocampus, midbrain, hindbrain, and cerebellum. The SPM analysis also showed an interaction between the illumination condition and the sex of the animal. Mouse activity was significantly different (p = 0.01) between light conditions (632 ± 215 s of movement) and dark conditions (989 ± 200 s), without significant effect of sex (p = 0.416). We concluded that room illumination conditions during [18F]FDG uptake in mice affected the brain [18F]FDG biodistribution. Therefore, we highlight the importance to control this factor to ensure more reliable and reproducible mouse brain [18F]FDG-PET results.
format	article
author	Alexandra de Francisco Yolanda Sierra-Palomares María Felipe Daniel Calle Manuel Desco Lorena Cussó
author_facet	Alexandra de Francisco Yolanda Sierra-Palomares María Felipe Daniel Calle Manuel Desco Lorena Cussó
author_sort	Alexandra de Francisco
title	Effect of illumination level [18F]FDG-PET brain uptake in free moving mice.
title_short	Effect of illumination level [18F]FDG-PET brain uptake in free moving mice.
title_full	Effect of illumination level [18F]FDG-PET brain uptake in free moving mice.
title_fullStr	Effect of illumination level [18F]FDG-PET brain uptake in free moving mice.
title_full_unstemmed	Effect of illumination level [18F]FDG-PET brain uptake in free moving mice.
title_sort	effect of illumination level [18f]fdg-pet brain uptake in free moving mice.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5ad98df99f3546d1ad24d448fee8d5a9
work_keys_str_mv	AT alexandradefrancisco effectofilluminationlevel18ffdgpetbrainuptakeinfreemovingmice AT yolandasierrapalomares effectofilluminationlevel18ffdgpetbrainuptakeinfreemovingmice AT mariafelipe effectofilluminationlevel18ffdgpetbrainuptakeinfreemovingmice AT danielcalle effectofilluminationlevel18ffdgpetbrainuptakeinfreemovingmice AT manueldesco effectofilluminationlevel18ffdgpetbrainuptakeinfreemovingmice AT lorenacusso effectofilluminationlevel18ffdgpetbrainuptakeinfreemovingmice
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Effect of illumination level [18F]FDG-PET brain uptake in free moving mice.

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