Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding

Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods. Here, the authors design a photo-switchable nanogel exhibiting variable fluorescenc...

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Autores principales:	Yujie Xie, Maria C. Arno, Jonathan T. Husband, Miquel Torrent-Sucarrat, Rachel K. O’Reilly
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5add959a2768499eb2e91d759b5ad720
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id	oai:doaj.org-article:5add959a2768499eb2e91d759b5ad720
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spelling	oai:doaj.org-article:5add959a2768499eb2e91d759b5ad7202021-12-02T15:45:25ZManipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding10.1038/s41467-020-16297-32041-1723https://doaj.org/article/5add959a2768499eb2e91d759b5ad7202020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16297-3https://doaj.org/toc/2041-1723Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods. Here, the authors design a photo-switchable nanogel exhibiting variable fluorescence lifetime, and demonstrate visual mapping by using fluorescence lifetime imaging microscopy on a sub-cellular scale.Yujie XieMaria C. ArnoJonathan T. HusbandMiquel Torrent-SucarratRachel K. O’ReillyNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yujie Xie Maria C. Arno Jonathan T. Husband Miquel Torrent-Sucarrat Rachel K. O’Reilly Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
description	Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods. Here, the authors design a photo-switchable nanogel exhibiting variable fluorescence lifetime, and demonstrate visual mapping by using fluorescence lifetime imaging microscopy on a sub-cellular scale.
format	article
author	Yujie Xie Maria C. Arno Jonathan T. Husband Miquel Torrent-Sucarrat Rachel K. O’Reilly
author_facet	Yujie Xie Maria C. Arno Jonathan T. Husband Miquel Torrent-Sucarrat Rachel K. O’Reilly
author_sort	Yujie Xie
title	Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
title_short	Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
title_full	Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
title_fullStr	Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
title_full_unstemmed	Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
title_sort	manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5add959a2768499eb2e91d759b5ad720
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Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding

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