Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones

Currently available diagnostic procedures for infections are laborious and time-consuming, resulting in a substantial financial burden by increasing morbidity, increased costs of hospitalization, and mortality. Therefore, innovative approaches to design diagnostic biomarkers are imperative to assist...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sahana Vasudevan, Parthasarathy Srinivasan, Prasanna Neelakantan, John Bosco Balaguru Rayappan, Adline Princy Solomon
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	TiO2 nanoparticles quorum sensing acyl homoserine lactone Pseudomonas aeruginosa photoluminescence Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5adf41aa75fb41fe97278a8c3d79a747
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5adf41aa75fb41fe97278a8c3d79a747
record_format	dspace
spelling	oai:doaj.org-article:5adf41aa75fb41fe97278a8c3d79a7472021-12-03T06:09:54ZPhotoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones2296-418510.3389/fbioe.2021.750933https://doaj.org/article/5adf41aa75fb41fe97278a8c3d79a7472021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.750933/fullhttps://doaj.org/toc/2296-4185Currently available diagnostic procedures for infections are laborious and time-consuming, resulting in a substantial financial burden by increasing morbidity, increased costs of hospitalization, and mortality. Therefore, innovative approaches to design diagnostic biomarkers are imperative to assist in the rapid and sensitive diagnosis of microbial infections. Acyl homoserine lactones (AHLs) are ubiquitous bacterial signaling molecules that are found to be significantly upregulated in infected sites. In this pioneering work, we have developed a simple photoluminescence-based assay using cysteamine-capped titanium oxide (TiO2) nanoparticles for AHL detection. The PL intensity variation of the oxygen defect state of TiO2 was used for the biosensing measurements. The bioassays were validated using two well-studied AHL molecules (C4-HSL and 3-oxo-C12 HSL) of an important human pathogen, Pseudomonas aeruginosa. The developed system has a maximum relative response of 98%. Furthermore, the efficacy of the system in simulated host urine using an artificial urine medium showed a linear detection range of 10–160 nM. Also, we confirmed the relative response and specificity of the system in detecting AHLs produced by P. aeruginosa in a temporal manner.Sahana VasudevanParthasarathy SrinivasanParthasarathy SrinivasanPrasanna NeelakantanJohn Bosco Balaguru RayappanAdline Princy SolomonFrontiers Media S.A.articleTiO2 nanoparticlesquorum sensingacyl homoserine lactonePseudomonas aeruginosaphotoluminescenceBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TiO2 nanoparticles quorum sensing acyl homoserine lactone Pseudomonas aeruginosa photoluminescence Biotechnology TP248.13-248.65
spellingShingle	TiO2 nanoparticles quorum sensing acyl homoserine lactone Pseudomonas aeruginosa photoluminescence Biotechnology TP248.13-248.65 Sahana Vasudevan Parthasarathy Srinivasan Parthasarathy Srinivasan Prasanna Neelakantan John Bosco Balaguru Rayappan Adline Princy Solomon Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones
description	Currently available diagnostic procedures for infections are laborious and time-consuming, resulting in a substantial financial burden by increasing morbidity, increased costs of hospitalization, and mortality. Therefore, innovative approaches to design diagnostic biomarkers are imperative to assist in the rapid and sensitive diagnosis of microbial infections. Acyl homoserine lactones (AHLs) are ubiquitous bacterial signaling molecules that are found to be significantly upregulated in infected sites. In this pioneering work, we have developed a simple photoluminescence-based assay using cysteamine-capped titanium oxide (TiO2) nanoparticles for AHL detection. The PL intensity variation of the oxygen defect state of TiO2 was used for the biosensing measurements. The bioassays were validated using two well-studied AHL molecules (C4-HSL and 3-oxo-C12 HSL) of an important human pathogen, Pseudomonas aeruginosa. The developed system has a maximum relative response of 98%. Furthermore, the efficacy of the system in simulated host urine using an artificial urine medium showed a linear detection range of 10–160 nM. Also, we confirmed the relative response and specificity of the system in detecting AHLs produced by P. aeruginosa in a temporal manner.
format	article
author	Sahana Vasudevan Parthasarathy Srinivasan Parthasarathy Srinivasan Prasanna Neelakantan John Bosco Balaguru Rayappan Adline Princy Solomon
author_facet	Sahana Vasudevan Parthasarathy Srinivasan Parthasarathy Srinivasan Prasanna Neelakantan John Bosco Balaguru Rayappan Adline Princy Solomon
author_sort	Sahana Vasudevan
title	Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones
title_short	Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones
title_full	Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones
title_fullStr	Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones
title_full_unstemmed	Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones
title_sort	photoluminescence-based bioassay with cysteamine-capped tio2 nanoparticles for the selective recognition of n-acyl homoserine lactones
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5adf41aa75fb41fe97278a8c3d79a747
work_keys_str_mv	AT sahanavasudevan photoluminescencebasedbioassaywithcysteaminecappedtio2nanoparticlesfortheselectiverecognitionofnacylhomoserinelactones AT parthasarathysrinivasan photoluminescencebasedbioassaywithcysteaminecappedtio2nanoparticlesfortheselectiverecognitionofnacylhomoserinelactones AT parthasarathysrinivasan photoluminescencebasedbioassaywithcysteaminecappedtio2nanoparticlesfortheselectiverecognitionofnacylhomoserinelactones AT prasannaneelakantan photoluminescencebasedbioassaywithcysteaminecappedtio2nanoparticlesfortheselectiverecognitionofnacylhomoserinelactones AT johnboscobalagururayappan photoluminescencebasedbioassaywithcysteaminecappedtio2nanoparticlesfortheselectiverecognitionofnacylhomoserinelactones AT adlineprincysolomon photoluminescencebasedbioassaywithcysteaminecappedtio2nanoparticlesfortheselectiverecognitionofnacylhomoserinelactones
_version_	1718373873486921728

Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones

Ejemplares similares