Characterisation of dendritic cells arising from progenitors endogenous to murine spleen.

Heterogeneity amongst dendritic cell (DC) subsets leads to a spectrum of immune response capacity against pathogens. Several DC subsets in spleen have been described which differ in terms of phenotype and function. We have previously reported a distinct population of CD11c(lo)CD11b(hi)MHC-II(-)CD8(-...

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Autores principales:	Sawang Petvises, Helen C O'Neill
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ae2f553f1514bee8efc4434309f741d
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spelling	oai:doaj.org-article:5ae2f553f1514bee8efc4434309f741d2021-11-18T08:32:29ZCharacterisation of dendritic cells arising from progenitors endogenous to murine spleen.1932-620310.1371/journal.pone.0088311https://doaj.org/article/5ae2f553f1514bee8efc4434309f741d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24551086/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Heterogeneity amongst dendritic cell (DC) subsets leads to a spectrum of immune response capacity against pathogens. Several DC subsets in spleen have been described which differ in terms of phenotype and function. We have previously reported a distinct population of CD11c(lo)CD11b(hi)MHC-II(-)CD8(-) dendritic-like "L-DC" in murine spleen, which can also be generated in splenic stromal longterm cultures. Here, the ontogeny of L-DC development in perinatal mice has been compared with other known splenic DC subsets. Flow cytometric analysis has revealed the presence of L-DC at embryonic age (E)18.5 spleen, while plasmacytoid (p)DC and conventional (c)DC appear at 2 and 4 days following birth. Co-cultures of E18.5 spleen above splenic stroma also showed production of only L-DC, while spleen cells from D0 through D5 neonates showed production of both L-DC and cDC-like cells. Addition of an M-CSFR inhibitor to co-cultures revealed that while the development of cDC-like cells depended on M-CSF, many L-DC developed independently of M-CSF. Furthermore, purified hematopoietic stem cells (HSC) and multipotential progenitors (MPP) isolated from neonatal D1 spleen are capable of developing into L-DC in co-cultures. These studies reveal a lineage of dendritic-like cells developing in the spleen microenvironment, and which appear to arise from endogenous progenitors laid down in spleen during embryogenesis.Sawang PetvisesHelen C O'NeillPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88311 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sawang Petvises Helen C O'Neill Characterisation of dendritic cells arising from progenitors endogenous to murine spleen.
description	Heterogeneity amongst dendritic cell (DC) subsets leads to a spectrum of immune response capacity against pathogens. Several DC subsets in spleen have been described which differ in terms of phenotype and function. We have previously reported a distinct population of CD11c(lo)CD11b(hi)MHC-II(-)CD8(-) dendritic-like "L-DC" in murine spleen, which can also be generated in splenic stromal longterm cultures. Here, the ontogeny of L-DC development in perinatal mice has been compared with other known splenic DC subsets. Flow cytometric analysis has revealed the presence of L-DC at embryonic age (E)18.5 spleen, while plasmacytoid (p)DC and conventional (c)DC appear at 2 and 4 days following birth. Co-cultures of E18.5 spleen above splenic stroma also showed production of only L-DC, while spleen cells from D0 through D5 neonates showed production of both L-DC and cDC-like cells. Addition of an M-CSFR inhibitor to co-cultures revealed that while the development of cDC-like cells depended on M-CSF, many L-DC developed independently of M-CSF. Furthermore, purified hematopoietic stem cells (HSC) and multipotential progenitors (MPP) isolated from neonatal D1 spleen are capable of developing into L-DC in co-cultures. These studies reveal a lineage of dendritic-like cells developing in the spleen microenvironment, and which appear to arise from endogenous progenitors laid down in spleen during embryogenesis.
format	article
author	Sawang Petvises Helen C O'Neill
author_facet	Sawang Petvises Helen C O'Neill
author_sort	Sawang Petvises
title	Characterisation of dendritic cells arising from progenitors endogenous to murine spleen.
title_short	Characterisation of dendritic cells arising from progenitors endogenous to murine spleen.
title_full	Characterisation of dendritic cells arising from progenitors endogenous to murine spleen.
title_fullStr	Characterisation of dendritic cells arising from progenitors endogenous to murine spleen.
title_full_unstemmed	Characterisation of dendritic cells arising from progenitors endogenous to murine spleen.
title_sort	characterisation of dendritic cells arising from progenitors endogenous to murine spleen.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5ae2f553f1514bee8efc4434309f741d
work_keys_str_mv	AT sawangpetvises characterisationofdendriticcellsarisingfromprogenitorsendogenoustomurinespleen AT helenconeill characterisationofdendriticcellsarisingfromprogenitorsendogenoustomurinespleen
_version_	1718421684362412032

Characterisation of dendritic cells arising from progenitors endogenous to murine spleen.

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