Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.

Infectious salmon anaemia virus (ISAV) is the cause of an important waterborne disease of farmed Atlantic salmon. Detection of virus in water samples may constitute an alternative method to sacrificing fish for surveillance of fish populations for the presence of ISA-virus. We aimed to evaluate diff...

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Autores principales:	Simon Chioma Weli, Haitham Tartor, Bjørn Spilsberg, Ole Bendik Dale, Atle Lillehaug
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5aea3e9baf3e483f98dd9a65c3085f81
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spelling	oai:doaj.org-article:5aea3e9baf3e483f98dd9a65c3085f812021-12-02T20:10:32ZShort communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.1932-620310.1371/journal.pone.0253297https://doaj.org/article/5aea3e9baf3e483f98dd9a65c3085f812021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253297https://doaj.org/toc/1932-6203Infectious salmon anaemia virus (ISAV) is the cause of an important waterborne disease of farmed Atlantic salmon. Detection of virus in water samples may constitute an alternative method to sacrificing fish for surveillance of fish populations for the presence of ISA-virus. We aimed to evaluate different membrane filters and buffers for concentration and recovery of ISAV in seawater, prior to molecular detection. One litre each of artificial and natural seawater was spiked with ISAV, followed by concentration with different filters and subsequent elution with different buffers. The negatively charged MF hydrophilic membrane filter, combined with NucliSENS® lysis buffer, presented the highest ISAV recovery percentages with 12.5 ± 1.3% by RT-qPCR and 31.7 ± 10.7% by RT-ddPCR. For the positively charged 1 MDS Zeta Plus® Virosorb® membrane filter, combined with NucliSENS® lysis buffer, the ISAV recovery percentages were 3.4 ± 0.1% by RT-qPCR and 10.8 ± 14.2% by RT-ddPCR. The limits of quantification (LOQ) were estimated to be 2.2 x 103 ISAV copies/L of natural seawater for both RT-qPCR and RT-ddPCR. The ISAV concentration method was more efficient in natural seawater.Simon Chioma WeliHaitham TartorBjørn SpilsbergOle Bendik DaleAtle LillehaugPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253297 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simon Chioma Weli Haitham Tartor Bjørn Spilsberg Ole Bendik Dale Atle Lillehaug Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.
description	Infectious salmon anaemia virus (ISAV) is the cause of an important waterborne disease of farmed Atlantic salmon. Detection of virus in water samples may constitute an alternative method to sacrificing fish for surveillance of fish populations for the presence of ISA-virus. We aimed to evaluate different membrane filters and buffers for concentration and recovery of ISAV in seawater, prior to molecular detection. One litre each of artificial and natural seawater was spiked with ISAV, followed by concentration with different filters and subsequent elution with different buffers. The negatively charged MF hydrophilic membrane filter, combined with NucliSENS® lysis buffer, presented the highest ISAV recovery percentages with 12.5 ± 1.3% by RT-qPCR and 31.7 ± 10.7% by RT-ddPCR. For the positively charged 1 MDS Zeta Plus® Virosorb® membrane filter, combined with NucliSENS® lysis buffer, the ISAV recovery percentages were 3.4 ± 0.1% by RT-qPCR and 10.8 ± 14.2% by RT-ddPCR. The limits of quantification (LOQ) were estimated to be 2.2 x 103 ISAV copies/L of natural seawater for both RT-qPCR and RT-ddPCR. The ISAV concentration method was more efficient in natural seawater.
format	article
author	Simon Chioma Weli Haitham Tartor Bjørn Spilsberg Ole Bendik Dale Atle Lillehaug
author_facet	Simon Chioma Weli Haitham Tartor Bjørn Spilsberg Ole Bendik Dale Atle Lillehaug
author_sort	Simon Chioma Weli
title	Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.
title_short	Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.
title_full	Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.
title_fullStr	Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.
title_full_unstemmed	Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.
title_sort	short communication: evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5aea3e9baf3e483f98dd9a65c3085f81
work_keys_str_mv	AT simonchiomaweli shortcommunicationevaluationofchargedmembranefiltersandbuffersforconcentrationandrecoveryofinfectioussalmonanaemiavirusinseawater AT haithamtartor shortcommunicationevaluationofchargedmembranefiltersandbuffersforconcentrationandrecoveryofinfectioussalmonanaemiavirusinseawater AT bjørnspilsberg shortcommunicationevaluationofchargedmembranefiltersandbuffersforconcentrationandrecoveryofinfectioussalmonanaemiavirusinseawater AT olebendikdale shortcommunicationevaluationofchargedmembranefiltersandbuffersforconcentrationandrecoveryofinfectioussalmonanaemiavirusinseawater AT atlelillehaug shortcommunicationevaluationofchargedmembranefiltersandbuffersforconcentrationandrecoveryofinfectioussalmonanaemiavirusinseawater
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Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater.

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