Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Zhizeng Wang,1 Dejuan Zhi,2 Yang Zhao,1 Hailong Zhang,2 Xin Wang,2 Yi Ru,1 Hongyu Li1,2 1MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, People's Republic of China; 2Institute of Microbiology and Biochemical Pharmacy, Schoo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang ZZ, Zhi DJ, Zhao Y, Zhang HL, Wang X, Ru Y, Li HY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5aeb31e649984856a3b0fda691334c2a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5aeb31e649984856a3b0fda691334c2a
record_format	dspace
spelling	oai:doaj.org-article:5aeb31e649984856a3b0fda691334c2a2021-12-02T00:38:49ZLateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed1178-2013https://doaj.org/article/5aeb31e649984856a3b0fda691334c2a2014-04-01T00:00:00Zhttp://www.dovepress.com/lateral-flow-test-strip-based-on-colloidal-selenium-immunoassay-for-ra-a16333https://doaj.org/toc/1178-2013 Zhizeng Wang,1 Dejuan Zhi,2 Yang Zhao,1 Hailong Zhang,2 Xin Wang,2 Yi Ru,1 Hongyu Li1,2 1MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, People's Republic of China; 2Institute of Microbiology and Biochemical Pharmacy, School of Pharmaceutics, Lanzhou University, Lanzhou, People's Republic of China Abstract: Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory method of detecting MEL in onsite or in-home testing is in urgent need of development. This work aimed to explore a rapid, convenient, and cost-effective method of identifying MEL in milk products or other food by colloidal selenium-based lateral flow immunoassay. Colloidal selenium was synthesized by L-ascorbic acid to reduce seleninic acid at room temperature. After conjugation with a monoclonal antibody anti-MEL, a test strip was successfully prepared. The detection limit of the test strip reached 150 µg/kg, 1,000 µg/kg, and 800 µg/kg in liquid milk, milk powder, and animal feed, respectively. No cross-reactions with homologues cyanuric acid, cyanurodiamide, or ammelide were found. Moreover, the MEL test strip can remain stable after storage for 1 year at room temperature. Our results demonstrate that the colloidal selenium MEL test strip can detect MEL in adulterated milk products or animal feed conveniently, rapidly, and sensitively. In contrast with a colloidal gold MEL test strip, the colloidal selenium MEL test strip was easy to prepare and more cost-efficient. Keywords: melamine, selenium nanoparticles, test strip, milk, animal feed, dairy foodWang ZZZhi DJZhao YZhang HLWang XRu YLi HYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1699-1707 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang ZZ Zhi DJ Zhao Y Zhang HL Wang X Ru Y Li HY Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed
description	Zhizeng Wang,1 Dejuan Zhi,2 Yang Zhao,1 Hailong Zhang,2 Xin Wang,2 Yi Ru,1 Hongyu Li1,2 1MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, People's Republic of China; 2Institute of Microbiology and Biochemical Pharmacy, School of Pharmaceutics, Lanzhou University, Lanzhou, People's Republic of China Abstract: Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory method of detecting MEL in onsite or in-home testing is in urgent need of development. This work aimed to explore a rapid, convenient, and cost-effective method of identifying MEL in milk products or other food by colloidal selenium-based lateral flow immunoassay. Colloidal selenium was synthesized by L-ascorbic acid to reduce seleninic acid at room temperature. After conjugation with a monoclonal antibody anti-MEL, a test strip was successfully prepared. The detection limit of the test strip reached 150 µg/kg, 1,000 µg/kg, and 800 µg/kg in liquid milk, milk powder, and animal feed, respectively. No cross-reactions with homologues cyanuric acid, cyanurodiamide, or ammelide were found. Moreover, the MEL test strip can remain stable after storage for 1 year at room temperature. Our results demonstrate that the colloidal selenium MEL test strip can detect MEL in adulterated milk products or animal feed conveniently, rapidly, and sensitively. In contrast with a colloidal gold MEL test strip, the colloidal selenium MEL test strip was easy to prepare and more cost-efficient. Keywords: melamine, selenium nanoparticles, test strip, milk, animal feed, dairy food
format	article
author	Wang ZZ Zhi DJ Zhao Y Zhang HL Wang X Ru Y Li HY
author_facet	Wang ZZ Zhi DJ Zhao Y Zhang HL Wang X Ru Y Li HY
author_sort	Wang ZZ
title	Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed
title_short	Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed
title_full	Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed
title_fullStr	Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed
title_full_unstemmed	Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed
title_sort	lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/5aeb31e649984856a3b0fda691334c2a
work_keys_str_mv	AT wangzz lateralflowteststripbasedoncolloidalseleniumimmunoassayforrapiddetectionofmelamineinmilkmilkpowderandanimalfeed AT zhidj lateralflowteststripbasedoncolloidalseleniumimmunoassayforrapiddetectionofmelamineinmilkmilkpowderandanimalfeed AT zhaoy lateralflowteststripbasedoncolloidalseleniumimmunoassayforrapiddetectionofmelamineinmilkmilkpowderandanimalfeed AT zhanghl lateralflowteststripbasedoncolloidalseleniumimmunoassayforrapiddetectionofmelamineinmilkmilkpowderandanimalfeed AT wangx lateralflowteststripbasedoncolloidalseleniumimmunoassayforrapiddetectionofmelamineinmilkmilkpowderandanimalfeed AT ruy lateralflowteststripbasedoncolloidalseleniumimmunoassayforrapiddetectionofmelamineinmilkmilkpowderandanimalfeed AT lihy lateralflowteststripbasedoncolloidalseleniumimmunoassayforrapiddetectionofmelamineinmilkmilkpowderandanimalfeed
_version_	1718403556289019904

Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Ejemplares similares