Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification

An ultrasensitive and selective method for Bisphenol A (BPA) detection using aptamer-BPA and real-time quantitative polymerase chain reaction (RT-qPCR) technology is reported, in which biotin-modified aptamer DNA (BPA) is fixed on the inner wall of a streptavidin-coated PCR tube via biotin–avidin in...

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Autores principales:	Yingyue Zhu, Xiangyuan Gu, Feng Jiang, Rui Jia, Mengyi Jin, Menglin Chen, Genhua Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	bisphenol a aptamer dna real-time quantitative polymerase chain reaction cycle threshold Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/5af1a298a402444085c18857251bd1b8
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spelling	oai:doaj.org-article:5af1a298a402444085c18857251bd1b82021-11-26T11:19:47ZUltrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification0954-01051465-344310.1080/09540105.2018.1515893https://doaj.org/article/5af1a298a402444085c18857251bd1b82018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1515893https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443An ultrasensitive and selective method for Bisphenol A (BPA) detection using aptamer-BPA and real-time quantitative polymerase chain reaction (RT-qPCR) technology is reported, in which biotin-modified aptamer DNA (BPA) is fixed on the inner wall of a streptavidin-coated PCR tube via biotin–avidin interactions and the template is fixed through the complementary base pairing with aptamer DNA. Upon the addition of BPA, the template DNA could be off from the inner wall of PCR tube, which results from aptamer DNA interacted specifically with BPA. Thus, the number of template DNA change result from the BPA concentration is readily seen by cycle threshold (Ct) values. The sensor exhibited an excellent linear response between Ct values and BPA concentration in the range of 1–500 nM and the detection limit of this method for aqueous BPA was as low as 0.7 nM. In addition, this method showed good selectivity for BPA over its analogues.Yingyue ZhuXiangyuan GuFeng JiangRui JiaMengyi JinMenglin ChenGenhua ZhangTaylor & Francis Grouparticlebisphenol aaptamer dnareal-time quantitative polymerase chain reactioncycle thresholdAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1106-1115 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	bisphenol a aptamer dna real-time quantitative polymerase chain reaction cycle threshold Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	bisphenol a aptamer dna real-time quantitative polymerase chain reaction cycle threshold Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Yingyue Zhu Xiangyuan Gu Feng Jiang Rui Jia Mengyi Jin Menglin Chen Genhua Zhang Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification
description	An ultrasensitive and selective method for Bisphenol A (BPA) detection using aptamer-BPA and real-time quantitative polymerase chain reaction (RT-qPCR) technology is reported, in which biotin-modified aptamer DNA (BPA) is fixed on the inner wall of a streptavidin-coated PCR tube via biotin–avidin interactions and the template is fixed through the complementary base pairing with aptamer DNA. Upon the addition of BPA, the template DNA could be off from the inner wall of PCR tube, which results from aptamer DNA interacted specifically with BPA. Thus, the number of template DNA change result from the BPA concentration is readily seen by cycle threshold (Ct) values. The sensor exhibited an excellent linear response between Ct values and BPA concentration in the range of 1–500 nM and the detection limit of this method for aqueous BPA was as low as 0.7 nM. In addition, this method showed good selectivity for BPA over its analogues.
format	article
author	Yingyue Zhu Xiangyuan Gu Feng Jiang Rui Jia Mengyi Jin Menglin Chen Genhua Zhang
author_facet	Yingyue Zhu Xiangyuan Gu Feng Jiang Rui Jia Mengyi Jin Menglin Chen Genhua Zhang
author_sort	Yingyue Zhu
title	Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification
title_short	Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification
title_full	Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification
title_fullStr	Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification
title_full_unstemmed	Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification
title_sort	ultrasensitive detection of bisphenol a based on an aptasensor with dna amplification
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/5af1a298a402444085c18857251bd1b8
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Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification

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