Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules
Abstract The glycocalyx regulates the interaction of mammalian cells with extracellular molecules, such as cytokines. However, it is unknown to which extend the glycocalyx of distinct cancer cells control the binding and uptake of nanoparticles. In the present study, exome sequencing data of cancer...
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Nature Portfolio
2020
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oai:doaj.org-article:5af9a0004fc9430ab9c6775511b5e2262021-12-02T13:46:37ZDifferences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules10.1038/s41598-020-79882-y2045-2322https://doaj.org/article/5af9a0004fc9430ab9c6775511b5e2262020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79882-yhttps://doaj.org/toc/2045-2322Abstract The glycocalyx regulates the interaction of mammalian cells with extracellular molecules, such as cytokines. However, it is unknown to which extend the glycocalyx of distinct cancer cells control the binding and uptake of nanoparticles. In the present study, exome sequencing data of cancer patients and analysis of distinct melanoma and bladder cancer cell lines suggested differences in cancer cell-exposed glycocalyx components such as heparan sulphate. Our data indicate that glycocalyx differences affected the binding of cationic chitosan nanocapsules (Chi-NCs). The pronounced glycocalyx of bladder cancer cells enhanced the internalisation of nanoencapsulated capsaicin. Consequently, capsaicin induced apoptosis in the cancer cells, but not in the less glycosylated benign urothelial cells. Moreover, we measured counterion condensation on highly negatively charged heparan sulphate chains. Counterion condensation triggered a cooperative binding of Chi-NCs, characterised by a weak binding rate at low Chi-NC doses and a strongly increased binding rate at high Chi-NC concentrations. Our results indicate that the glycocalyx of tumour cells controls the binding and biological activity of nanoparticles. This has to be considered for the design of tumour cell directed nanocarriers to improve the delivery of cytotoxic drugs. Differential nanoparticle binding may also be useful to discriminate tumour cells from healthy cells.Lydia von PalubitzkiYuanyuan WangStefan HoffmannSabine Vidal-y-SyBernd ZobiakAntonio V. FaillaPetra SchmageAxel JohnAnayancy Osorio-MadrazoAlexander T. BauerStefan W. SchneiderFrancisco M. GoycooleaChristian GorzelannyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) |
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Medicine R Science Q Lydia von Palubitzki Yuanyuan Wang Stefan Hoffmann Sabine Vidal-y-Sy Bernd Zobiak Antonio V. Failla Petra Schmage Axel John Anayancy Osorio-Madrazo Alexander T. Bauer Stefan W. Schneider Francisco M. Goycoolea Christian Gorzelanny Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules |
| description |
Abstract The glycocalyx regulates the interaction of mammalian cells with extracellular molecules, such as cytokines. However, it is unknown to which extend the glycocalyx of distinct cancer cells control the binding and uptake of nanoparticles. In the present study, exome sequencing data of cancer patients and analysis of distinct melanoma and bladder cancer cell lines suggested differences in cancer cell-exposed glycocalyx components such as heparan sulphate. Our data indicate that glycocalyx differences affected the binding of cationic chitosan nanocapsules (Chi-NCs). The pronounced glycocalyx of bladder cancer cells enhanced the internalisation of nanoencapsulated capsaicin. Consequently, capsaicin induced apoptosis in the cancer cells, but not in the less glycosylated benign urothelial cells. Moreover, we measured counterion condensation on highly negatively charged heparan sulphate chains. Counterion condensation triggered a cooperative binding of Chi-NCs, characterised by a weak binding rate at low Chi-NC doses and a strongly increased binding rate at high Chi-NC concentrations. Our results indicate that the glycocalyx of tumour cells controls the binding and biological activity of nanoparticles. This has to be considered for the design of tumour cell directed nanocarriers to improve the delivery of cytotoxic drugs. Differential nanoparticle binding may also be useful to discriminate tumour cells from healthy cells. |
| format |
article |
| author |
Lydia von Palubitzki Yuanyuan Wang Stefan Hoffmann Sabine Vidal-y-Sy Bernd Zobiak Antonio V. Failla Petra Schmage Axel John Anayancy Osorio-Madrazo Alexander T. Bauer Stefan W. Schneider Francisco M. Goycoolea Christian Gorzelanny |
| author_facet |
Lydia von Palubitzki Yuanyuan Wang Stefan Hoffmann Sabine Vidal-y-Sy Bernd Zobiak Antonio V. Failla Petra Schmage Axel John Anayancy Osorio-Madrazo Alexander T. Bauer Stefan W. Schneider Francisco M. Goycoolea Christian Gorzelanny |
| author_sort |
Lydia von Palubitzki |
| title |
Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules |
| title_short |
Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules |
| title_full |
Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules |
| title_fullStr |
Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules |
| title_full_unstemmed |
Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules |
| title_sort |
differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/5af9a0004fc9430ab9c6775511b5e226 |
| work_keys_str_mv |
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