Photoacoustic imaging of voltage responses beyond the optical diffusion limit

Abstract Non-invasive optical imaging of neuronal voltage response signals in live brains is constrained in depth by the optical diffusion limit, which is due primarily to optical scattering by brain tissues. Although photoacoustic tomography breaks this limit by exciting the targets with diffused p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bin Rao, Ruiying Zhang, Lei Li, Jin-Yu Shao, Lihong V. Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5afae3d95d7346edae55c28b593bd5a6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5afae3d95d7346edae55c28b593bd5a6
record_format	dspace
spelling	oai:doaj.org-article:5afae3d95d7346edae55c28b593bd5a62021-12-02T12:30:15ZPhotoacoustic imaging of voltage responses beyond the optical diffusion limit10.1038/s41598-017-02458-w2045-2322https://doaj.org/article/5afae3d95d7346edae55c28b593bd5a62017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02458-whttps://doaj.org/toc/2045-2322Abstract Non-invasive optical imaging of neuronal voltage response signals in live brains is constrained in depth by the optical diffusion limit, which is due primarily to optical scattering by brain tissues. Although photoacoustic tomography breaks this limit by exciting the targets with diffused photons and detecting the resulting acoustic responses, it has not been demonstrated as a modality for imaging voltage responses. In this communication, we report the first demonstration of photoacoustic voltage response imaging in both in vitro HEK-293 cell cultures and in vivo mouse brain surfaces. Using spectroscopic photoacoustic tomography at isosbestic wavelengths, we can separate voltage response signals and hemodynamic signals on live brain surfaces. By imaging HEK-293 cell clusters through 4.5 mm thick ex vivo rat brain tissue, we demonstrate photoacoustic tomography of cell membrane voltage responses beyond the optical diffusion limit. Although the current voltage dye does not immediately allow in vivo deep brain voltage response imaging, we believe our method opens up a feasible technical path for deep brain studies in the future.Bin RaoRuiying ZhangLei LiJin-Yu ShaoLihong V. WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bin Rao Ruiying Zhang Lei Li Jin-Yu Shao Lihong V. Wang Photoacoustic imaging of voltage responses beyond the optical diffusion limit
description	Abstract Non-invasive optical imaging of neuronal voltage response signals in live brains is constrained in depth by the optical diffusion limit, which is due primarily to optical scattering by brain tissues. Although photoacoustic tomography breaks this limit by exciting the targets with diffused photons and detecting the resulting acoustic responses, it has not been demonstrated as a modality for imaging voltage responses. In this communication, we report the first demonstration of photoacoustic voltage response imaging in both in vitro HEK-293 cell cultures and in vivo mouse brain surfaces. Using spectroscopic photoacoustic tomography at isosbestic wavelengths, we can separate voltage response signals and hemodynamic signals on live brain surfaces. By imaging HEK-293 cell clusters through 4.5 mm thick ex vivo rat brain tissue, we demonstrate photoacoustic tomography of cell membrane voltage responses beyond the optical diffusion limit. Although the current voltage dye does not immediately allow in vivo deep brain voltage response imaging, we believe our method opens up a feasible technical path for deep brain studies in the future.
format	article
author	Bin Rao Ruiying Zhang Lei Li Jin-Yu Shao Lihong V. Wang
author_facet	Bin Rao Ruiying Zhang Lei Li Jin-Yu Shao Lihong V. Wang
author_sort	Bin Rao
title	Photoacoustic imaging of voltage responses beyond the optical diffusion limit
title_short	Photoacoustic imaging of voltage responses beyond the optical diffusion limit
title_full	Photoacoustic imaging of voltage responses beyond the optical diffusion limit
title_fullStr	Photoacoustic imaging of voltage responses beyond the optical diffusion limit
title_full_unstemmed	Photoacoustic imaging of voltage responses beyond the optical diffusion limit
title_sort	photoacoustic imaging of voltage responses beyond the optical diffusion limit
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5afae3d95d7346edae55c28b593bd5a6
work_keys_str_mv	AT binrao photoacousticimagingofvoltageresponsesbeyondtheopticaldiffusionlimit AT ruiyingzhang photoacousticimagingofvoltageresponsesbeyondtheopticaldiffusionlimit AT leili photoacousticimagingofvoltageresponsesbeyondtheopticaldiffusionlimit AT jinyushao photoacousticimagingofvoltageresponsesbeyondtheopticaldiffusionlimit AT lihongvwang photoacousticimagingofvoltageresponsesbeyondtheopticaldiffusionlimit
_version_	1718394399699763200

Photoacoustic imaging of voltage responses beyond the optical diffusion limit

Ejemplares similares