AID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>

ABSTRACT The cyclic GMP-dependent protein kinase (PKG) of apicomplexan parasites is essential for secretion of micronemes and host cell invasion and egress. Both kinase specificity and localization can determine which substrates are phosphorylated. The functions of plasma membrane and cytosolic PKG...

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Autor principal:	Kami Kim
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Toxoplasma cell signaling gene regulation genetics kinases Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5b063e4212084114acb83805bf64143d
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spelling	oai:doaj.org-article:5b063e4212084114acb83805bf64143d2021-11-15T15:51:44ZAID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>10.1128/mBio.01076-172150-7511https://doaj.org/article/5b063e4212084114acb83805bf64143d2017-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01076-17https://doaj.org/toc/2150-7511ABSTRACT The cyclic GMP-dependent protein kinase (PKG) of apicomplexan parasites is essential for secretion of micronemes and host cell invasion and egress. Both kinase specificity and localization can determine which substrates are phosphorylated. The functions of plasma membrane and cytosolic PKG isoforms of Toxoplasma gondii were unknown because of difficulties precisely manipulating expression of essential genes. Brown et al. (K. M. Brown, S. Long, and L. D. Sibley, mBio 8:e00375-17, https://doi.org/10.1128/mBio.00375-17 ) adapted the auxin-inducible degron (AID) system for conditional expression of T. gondii proteins. AID, in combination with clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 gene editing, facilitated creation of a panel of PKG mutants to demonstrate that the membrane association via acylation of PKG is critical for its essential functions in tachyzoites. The cytosolic form of PKG is not sufficient for viability and is dispensable. These studies illuminate a critical role for targeting of kinase complexes for parasite viability. The AID system enables rapid, conditional regulation of protein expression that expands the molecular toolbox of T. gondii.Kami KimAmerican Society for MicrobiologyarticleToxoplasmacell signalinggene regulationgeneticskinasesMicrobiologyQR1-502ENmBio, Vol 8, Iss 4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Toxoplasma cell signaling gene regulation genetics kinases Microbiology QR1-502
spellingShingle	Toxoplasma cell signaling gene regulation genetics kinases Microbiology QR1-502 Kami Kim AID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>
description	ABSTRACT The cyclic GMP-dependent protein kinase (PKG) of apicomplexan parasites is essential for secretion of micronemes and host cell invasion and egress. Both kinase specificity and localization can determine which substrates are phosphorylated. The functions of plasma membrane and cytosolic PKG isoforms of Toxoplasma gondii were unknown because of difficulties precisely manipulating expression of essential genes. Brown et al. (K. M. Brown, S. Long, and L. D. Sibley, mBio 8:e00375-17, https://doi.org/10.1128/mBio.00375-17 ) adapted the auxin-inducible degron (AID) system for conditional expression of T. gondii proteins. AID, in combination with clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 gene editing, facilitated creation of a panel of PKG mutants to demonstrate that the membrane association via acylation of PKG is critical for its essential functions in tachyzoites. The cytosolic form of PKG is not sufficient for viability and is dispensable. These studies illuminate a critical role for targeting of kinase complexes for parasite viability. The AID system enables rapid, conditional regulation of protein expression that expands the molecular toolbox of T. gondii.
format	article
author	Kami Kim
author_facet	Kami Kim
author_sort	Kami Kim
title	AID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>
title_short	AID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>
title_full	AID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>
title_fullStr	AID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>
title_full_unstemmed	AID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>
title_sort	aid-ing signaling in <italic toggle="yes">toxoplasma gondii</italic>
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/5b063e4212084114acb83805bf64143d
work_keys_str_mv	AT kamikim aidingsignalinginitalictoggleyestoxoplasmagondiiitalic
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AID-ing Signaling in <italic toggle="yes">Toxoplasma gondii</italic>

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