A proteomics protocol to identify stimulation-induced binding partners dependent on a specific gene in mammalian cells
Summary: Some protein-protein interactions are induced by different kinds of stimulation and are dependent on specific genes. To identify these interaction partners, we present a protocol which utilizes affinity purification of Flag-tagged protein complexes followed by mass-spectrometry-based proteo...
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| Auteurs principaux: | , |
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| Format: | article |
| Langue: | EN |
| Publié: |
Elsevier
2021
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| Sujets: | |
| Accès en ligne: | https://doaj.org/article/5b067d16d3214e3b9d2f7d55f45809db |
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